Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: DNA adenine methylase


DESCRIPTION:
Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. Although it shares sequence specificity with a number of type II restriction endonucleases and methylases, it is thought to act in postreplication mismatch repair rather than as a part of a restriction modification system. May also play a role in DNA replication.

STRUCTURE SIMILARITY:
Belongs to the N(4)/N(6)-methyltransferase family.


CATALYTIC ACTIVITY:
S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.


PROTEIN TYPE(S):
DNA methyltransferase


RELATED PATHWAY(S):
DNA replication
mismatch repair (MMR)


Amino acids sequence

        10         20         30         40         50         60
MKKNRAFLKW AGGKYPLLDD IKRHLPKGEC LVEPFVGAGS VFLNTDFSRY ILADINSDLI
        70         80         90        100        110        120
SLYNIVKMRT DEYVQAAREL FVPETNCAEV YYQFREEFNK SQDPFRRAVL FLYLNRYGYN
       130        140        150        160        170        180
GLCRYNLRGE FNVPFGRYKK PYFPEAELYH FAEKAQNAFF YCESYADSMA RADDASVVYC
       190        200        210        220        230        240
DPPYAPLSAT ANFTAYHTNS FTLEQQAHLA EIAEGLVERH IPVLISNHDT MLTREWYQRA
       250        260        270
KLHVVKVRRS ISSNGGTRKK VDELLALYKP GVVSPAKK  

Encoded by dam gene


OTHER NAME(S):
ECK3374
JW3350


DESCRIPTION:
Dam is a base flipping enzyme. [More information is available at EcoGene: EG10204]. DNA adenine methyltransferase (Dam), which is responsible for methylation of nearly all GATC sequences in Escherichia coli , is encoded by the dam gene and occurs at about 130 molecules per rapidly growing cell . [More information is available at EcoCyc: EG10204].


Nucleic acid sequence

        10         20         30         40         50         60
atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat
        70         80         90        100        110        120
attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg
       130        140        150        160        170        180
gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag cgacctgatc
       190        200        210        220        230        240
agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagctg
       250        260        270        280        290        300
tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa
       310        320        330        340        350        360
agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac
       370        380        390        400        410        420
ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgttcggccg ctacaaaaaa
       430        440        450        460        470        480
ccctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc
       490        500        510        520        530        540
tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt cgtctattgc
       550        560        570        580        590        600
gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca cacaaacagt
       610        620        630        640        650        660
tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt tgagcgccat
       670        680        690        700        710        720
attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca
       730        740        750        760        770        780
aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag
       790        800        810        820        830
gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaataa

Last modification date: Oct. 2, 2011