Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: Replicative DNA helicase


DESCRIPTION:
Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins.

STRUCTURE SIMILARITY:
Contains 1 SF4 helicase domain.
Belongs to the helicase family. DnaB subfamily.


CATALYTIC ACTIVITY:
ATP + H(2)O = ADP + phosphate.


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MAGNKPFNKQ QAEPRERDPQ VAGLKVPPHS IEAEQSVLGG LMLDNERWDD VAERVVADDF
        70         80         90        100        110        120
YTRPHRHIFT EMARLQESGS PIDLITLAES LERQGQLDSV GGFAYLAELS KNTPSAANIS
       130        140        150        160        170        180
AYADIVRERA VVREMISVAN EIAEAGFDPQ GRTSEDLLDL AESRVFKIAE SRANKDEGPK
       190        200        210        220        230        240
NIADVLDATV ARIEQLFQQP HDGVTGVNTG YDDLNKKTAG LQPSDLIIVA ARPSMGKTTF
       250        260        270        280        290        300
AMNLVENAAM LQDKPVLIFS LEMPSEQIMM RSLASLSRVD QTKIRTGQLD DEDWARISGT
       310        320        330        340        350        360
MGILLEKRNI YIDDSSGLTP TEVRSRARRI AREHGGIGLI MIDYLQLMRV PALSDNRTLE
       370        380        390        400        410        420
IAEISRSLKA LAKELNVPVV ALSQLNRSLE QRADKRPVNS DLRESGSIEQ DADLIMFIYR
       430        440        450        460        470
DEVYHENSDL KGIAEIIIGK QRNGPIGTVR LTFNGQWSRF DNYAGPQYDD E

Encoded by dnaB gene


OTHER NAME(S):
ECK4044
groP
grpA
grpD
JW4012


DESCRIPTION:
The dnaB gene is essential. [More information is available at EcoGene: EG10236]. DnaB, the replicative DNA helicase, processively unwinds DNA at replication forks in advance of DNA polymerase. [More information is available at EcoCyc: EG10236].


Nucleic acid sequence

        10         20         30         40         50         60
atggcaggaa ataaaccctt caacaaacag caggctgaac cccgcgaacg cgatccacaa
        70         80         90        100        110        120
gttgccgggc tgaaagtgcc tccgcactcg atcgaagcgg agcagtcggt gttgggcggt
       130        140        150        160        170        180
ttaatgctag ataacgaacg ctgggatgat gtagccgagc gtgtggtagc agacgatttt
       190        200        210        220        230        240
tacacccgcc cacaccgtca tatctttact gaaatggcgc gtttgcagga aagcggtagc
       250        260        270        280        290        300
cctatcgatc tgattactct tgcggaatcg ctggaacgcc aggggcaact cgatagcgtc
       310        320        330        340        350        360
ggtggttttg cttatctggc agagctgtca aaaaatacgc caagtgcggc taacatcagt
       370        380        390        400        410        420
gcctatgcgg acatcgtgcg tgaacgtgcc gttgtccgtg agatgatctc ggttgcgaat
       430        440        450        460        470        480
gagattgccg aagctggttt tgatccgcag gggcgtacca gcgaagatct gctggatctg
       490        500        510        520        530        540
gctgaatccc gcgtctttaa aattgccgaa agtcgtgcga acaaagacga agggccgaag
       550        560        570        580        590        600
aacatcgccg atgtgctcga cgcaaccgtg gcgcgtattg agcagttgtt tcagcagcca
       610        620        630        640        650        660
cacgatggcg ttaccggggt aaacaccggt tatgacgatc tcaacaaaaa aaccgctggc
       670        680        690        700        710        720
ttgcagccgt cggatttgat catcgtcgcc gcgcgtccgt cgatgggtaa aacaacattt
       730        740        750        760        770        780
gcgatgaacc tcgtcgaaaa cgcggcgatg ttgcaggata aaccggtact tatcttctcg
       790        800        810        820        830        840
ctggagatgc catcagaaca gatcatgatg cgttctctgg cgtcgctgtc gcgcgttgac
       850        860        870        880        890        900
cagactaaaa tccgtaccgg gcagctcgat gacgaagact gggcgcgcat ttccggcacc
       910        920        930        940        950        960
atgggtattt tgctcgaaaa acgcaatatc tatatcgatg actcctccgg cctgacgcca
       970        980        990       1000       1010       1020
acggaagtgc gttcccgcgc acgccgtatt gcccgtgaac acggcggcat cgggcttatc
      1030       1040       1050       1060       1070       1080
atgatcgact acctgcaact gatgcgcgta ccggcgcttt ccgataaccg tacgctggaa
      1090       1100       1110       1120       1130       1140
attgcagaaa tctctcgctc gctgaaagca ctggcgaaag aactgaacgt gccggtggtg
      1150       1160       1170       1180       1190       1200
gcgctgtccc agttgaaccg ttctctggaa caacgtgccg acaaacgccc ggtcaactcc
      1210       1220       1230       1240       1250       1260
gacctgcgtg aatctggctc tatcgagcag gatgcggact tgatcatgtt tatctatcgt
      1270       1280       1290       1300       1310       1320
gatgaggtgt atcacgaaaa cagtgattta aaaggcatcg cggaaattat tatcggtaaa
      1330       1340       1350       1360       1370       1380
caacgtaacg gcccaatcgg gacggtacgc ctgaccttta acggtcaatg gtcgcgcttc
      1390       1400       1410
gacaactatg cggggccgca gtacgacgac gaataa  

Last modification date: Oct. 2, 2011