Homo sapiens L. (human) [HSA]

FULL NAME: Exonuclease 1


DESCRIPTION:
5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.

STRUCTURE SIMILARITY:
Belongs to the XPG/RAD2 endonuclease family. EXO1 subfamily.


POST-TRANSLATIONAL MODIFICATION:
Phosphorylated upon DNA damage and in response to agents stalling DNA replication, probably by ATM or ATR. Phosphorylation at Ser-454, Thr-621 and Ser-714 is induced upon DNA-damage caused by treatment with hydroxyurea (HU) but not upon IR treatment. The HU-induced EXO1 triple phosphorylation facilitates destabilisation/degradation of the protein.


PROTEIN TYPE(S):
5'-3' exonuclease
DNase
RNase


RELATED PATHWAY(S):
mismatch repair (MMR)


Amino acids sequence

        10         20         30         40         50         60
MGIQGLLQFI KEASEPIHVR KYKGQVVAVD TYCWLHKGAI ACAEKLAKGE PTDRYVGFCM
        70         80         90        100        110        120
KFVNMLLSHG IKPILVFDGC TLPSKKEVER SRRERRQANL LKGKQLLREG KVSEARECFT
       130        140        150        160        170        180
RSINITHAMA HKVIKAARSQ GVDCLVAPYE ADAQLAYLNK AGIVQAIITE DSDLLAFGCK
       190        200        210        220        230        240
KVILKMDQFG NGLEIDQARL GMCRQLGDVF TEEKFRYMCI LSGCDYLSSL RGIGLAKACK
       250        260        270        280        290        300
VLRLANNPDI VKVIKKIGHY LKMNITVPED YINGFIRANN TFLYQLVFDP IKRKLIPLNA
       310        320        330        340        350        360
YEDDVDPETL SYAGQYVDDS IALQIALGNK DINTFEQIDD YNPDTAMPAH SRSHSWDDKT
       370        380        390        400        410        420
CQKSANVSSI WHRNYSPRPE SGTVSDAPQL KENPSTVGVE RVISTKGLNL PRKSSIVKRP
       430        440        450        460        470        480
RSAELSEDDL LSQYSLSFTK KTKKNSSEGN KSLSFSEVFV PDLVNGPTNK KSVSTPPRTR
       490        500        510        520        530        540
NKFATFLQRK NEESGAVVVP GTRSRFFCSS DSTDCVSNKV SIQPLDETAV TDKENNLHES
       550        560        570        580        590        600
EYGDQEGKRL VDTDVARNSS DDIPNNHIPG DHIPDKATVF TDEESYSFES SKFTRTISPP
       610        620        630        640        650        660
TLGTLRSCFS WSGGLGDFSR TPSPSPSTAL QQFRRKSDSP TSLPENNMSD VSQLKSEESS
       670        680        690        700        710        720
DDESHPLREE ACSSQSQESG EFSLQSSNAS KLSQCSSKDS DSEESDCNIK LLDSQSDQTS
       730        740        750        760        770        780
KLRLSHFSKK DTPLRNKVPG LYKSSSADSL STTKIKPLGP ARASGLSKKP ASIQKRKHHN
       790        800
AENKPGLQIK LNELWKNFGF KKF   

Encoded by EXO1 gene

FULL NAME: exonuclease 1


OTHER NAME(S):
HEX1
hExoI


DESCRIPTION:
This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms. [provided by RefSeq, Jul 2008]


Nucleic acid sequence

        10         20         30         40         50         60
atggggatac agggattgct acaatttatc aaagaagctt cagaacccat ccatgtgagg
        70         80         90        100        110        120
aagtataaag ggcaggtagt agctgtggat acatattgct ggcttcacaa aggagctatt
       130        140        150        160        170        180
gcttgtgctg aaaaactagc caaaggtgaa cctactgata ggtatgtagg attttgtatg
       190        200        210        220        230        240
aaatttgtaa atatgttact atctcatggg atcaagccta ttctcgtatt tgatggatgt
       250        260        270        280        290        300
actttacctt ctaaaaagga agtagagaga tctagaagag aaagacgaca agccaatctt
       310        320        330        340        350        360
cttaagggaa agcaacttct tcgtgagggg aaagtctcgg aagctcgaga gtgtttcacc
       370        380        390        400        410        420
cggtctatca atatcacaca tgccatggcc cacaaagtaa ttaaagctgc ccggtctcag
       430        440        450        460        470        480
ggggtagatt gcctcgtggc tccctatgaa gctgatgcgc agttggccta tcttaacaaa
       490        500        510        520        530        540
gcgggaattg tgcaagccat aattacagag gactcggatc tcctagcttt tggctgtaaa
       550        560        570        580        590        600
aaggtaattt taaagatgga ccagtttgga aatggacttg aaattgatca agctcggcta
       610        620        630        640        650        660
ggaatgtgca gacagcttgg ggatgtattc acggaagaga agtttcgtta catgtgtatt
       670        680        690        700        710        720
ctttcaggtt gtgactacct gtcatcactg cgtgggattg gattagcaaa ggcatgcaaa
       730        740        750        760        770        780
gtcctaagac tagccaataa tccagatata gtaaaggtta tcaagaaaat tggacattat
       790        800        810        820        830        840
ctcaagatga atatcacggt accagaggat tacatcaacg ggtttattcg ggccaacaat
       850        860        870        880        890        900
accttcctct atcagctagt ttttgatccc atcaaaagga aacttattcc tctgaacgcc
       910        920        930        940        950        960
tatgaagatg atgttgatcc tgaaacacta agctacgctg ggcaatatgt tgatgattcc
       970        980        990       1000       1010       1020
atagctcttc aaatagcact tggaaataaa gatataaata cttttgaaca gatcgatgac
      1030       1040       1050       1060       1070       1080
tacaatccag acactgctat gcctgcccat tcaagaagtc atagttggga tgacaaaaca
      1090       1100       1110       1120       1130       1140
tgtcaaaagt cagctaatgt tagcagcatt tggcatagga attactctcc cagaccagag
      1150       1160       1170       1180       1190       1200
tcgggtactg tttcagatgc cccacaattg aaggaaaatc caagtactgt gggagtggaa
      1210       1220       1230       1240       1250       1260
cgagtgatta gtactaaagg gttaaatctc ccaaggaaat catccattgt gaaaagacca
      1270       1280       1290       1300       1310       1320
agaagtgcag agctgtcaga agatgacctg ttgagtcagt attctctttc atttacgaag
      1330       1340       1350       1360       1370       1380
aagaccaaga aaaatagctc tgaaggcaat aaatcattga gcttttctga agtgtttgtg
      1390       1400       1410       1420       1430       1440
cctgacctgg taaatggacc tactaacaaa aagagtgtaa gcactccacc taggacgaga
      1450       1460       1470       1480       1490       1500
aataaatttg caacattttt acaaaggaaa aatgaagaaa gtggtgcagt tgtggttcca
      1510       1520       1530       1540       1550       1560
gggaccagaa gcaggttttt ttgcagttca gattctactg actgtgtatc aaacaaagtg
      1570       1580       1590       1600       1610       1620
agcatccagc ctctggatga aactgctgtc acagataaag agaacaatct gcatgaatca
      1630       1640       1650       1660       1670       1680
gagtatggag accaagaagg caagagactg gttgacacag atgtagcacg taattcaagt
      1690       1700       1710       1720       1730       1740
gatgacattc cgaataatca tattccaggt gatcatattc cagacaaggc aacagtgttt
      1750       1760       1770       1780       1790       1800
acagatgaag agtcctactc ttttgagagc agcaaattta caaggaccat ttcaccaccc
      1810       1820       1830       1840       1850       1860
actttgggaa cactaagaag ttgttttagt tggtctggag gtcttggaga tttttcaaga
      1870       1880       1890       1900       1910       1920
acgccgagcc cctctccaag cacagcattg cagcagttcc gaagaaagag cgattccccc
      1930       1940       1950       1960       1970       1980
acctctttgc ctgagaataa tatgtctgat gtgtcgcagt taaagagcga ggagtccagt
      1990       2000       2010       2020       2030       2040
gacgatgagt ctcatccctt acgagaagag gcatgttctt cacagtccca ggaaagtgga
      2050       2060       2070       2080       2090       2100
gaattctcac tgcagagttc aaatgcatca aagctttctc agtgctctag taaggactct
      2110       2120       2130       2140       2150       2160
gattcagagg aatctgattg caatattaag ttacttgaca gtcaaagtga ccagacctcc
      2170       2180       2190       2200       2210       2220
aagctacgtt tatctcattt ctcaaaaaaa gacacacctc taaggaacaa ggttcctggg
      2230       2240       2250       2260       2270       2280
ctatataagt ccagttctgc agactctctt tctacaacca agatcaaacc tctaggacct
      2290       2300       2310       2320       2330       2340
gccagagcca gtgggctgag caagaagccg gcaagcatcc agaagagaaa gcatcataat
      2350       2360       2370       2380       2390       2400
gccgagaaca agccggggtt acagatcaaa ctcaatgagc tctggaaaaa ctttggattt
      2410
aaaaaattct ga    

Last modification date: Oct. 2, 2011