Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: DNA polymerase III subunit beta


DESCRIPTION:
DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA.

CATALYTIC ACTIVITY:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).


PROTEIN TYPE(S):
accessory protein


RELATED PATHWAY(S):
DNA replication
mismatch repair (MMR)
homologous recombination (HR)


Amino acids sequence

        10         20         30         40         50         60
MKFTVEREHL LKPLQQVSGP LGGRPTLPIL GNLLLQVADG TLSLTGTDLE MEMVARVALV
        70         80         90        100        110        120
QPHEPGATTV PARKFFDICR GLPEGAEIAV QLEGERMLVR SGRSRFSLST LPAADFPNLD
       130        140        150        160        170        180
DWQSEVEFTL PQATMKRLIE ATQFSMAHQD VRYYLNGMLF ETEGEELRTV ATDGHRLAVC
       190        200        210        220        230        240
SMPIGQSLPS HSVIVPRKGV IELMRMLDGG DNPLRVQIGS NNIRAHVGDF IFTSKLVDGR
       250        260        270        280        290        300
FPDYRRVLPK NPDKHLEAGC DLLKQAFARA AILSNEKFRG VRLYVSENQL KITANNPEQE
       310        320        330        340        350        360
EAEEILDVTY SGAEMEIGFN VSYVLDVLNA LKCENVRMML TDSVSSVQIE DAASQSAAYV

VMPMRL     

Encoded by dnaN gene


OTHER NAME(S):
ECK3693
JW3678


DESCRIPTION:
The beta subunit of DNA polymerase III dimerizes to form the sliding clamp that links the core polymerase to DNA and allows processive DNA replication. [More information is available at EcoCyc: EG10242].


Nucleic acid sequence

        10         20         30         40         50         60
atgaaattta ccgtagaacg tgagcattta ttaaaaccgc tacaacaggt gagcggtccg
        70         80         90        100        110        120
ttaggtggtc gtcctacgct accgattctc ggtaatctgc tgttacaggt tgctgacggt
       130        140        150        160        170        180
acgttgtcgc tgaccggtac tgatctcgag atggaaatgg tggcacgtgt tgcgctggtt
       190        200        210        220        230        240
cagccacacg agccaggagc gacgaccgtt ccggcgcgca aattctttga tatctgccgt
       250        260        270        280        290        300
ggtctgcctg aaggcgcgga aattgccgtg cagctggaag gtgaacggat gctggtacgc
       310        320        330        340        350        360
tccgggcgta gccgtttttc gctgtctacc ctgccagcgg cggatttccc gaacctcgat
       370        380        390        400        410        420
gactggcaga gtgaagtcga atttaccctg ccgcaggcaa cgatgaagcg tctgattgaa
       430        440        450        460        470        480
gcgacccagt tttctatggc gcatcaggac gttcgctatt acttaaatgg tatgctgttt
       490        500        510        520        530        540
gaaaccgaag gtgaagaact gcgcaccgtg gcaaccgacg gccaccgtct ggcggtctgt
       550        560        570        580        590        600
tcaatgccaa ttggtcaatc tttgccaagc cattcggtga tcgtaccgcg taaaggcgtg
       610        620        630        640        650        660
attgaactga tgcgtatgct cgacggcggc gacaatccgc tgcgcgtaca gattggcagc
       670        680        690        700        710        720
aacaacattc gcgcccacgt tggcgacttt atcttcacct ccaaactggt ggatggtcgc
       730        740        750        760        770        780
ttcccggatt atcgccgcgt tctgccgaag aacccggaca aacatctgga agctggctgc
       790        800        810        820        830        840
gatctgctca agcaggcgtt tgctcgcgcg gcgattctct ctaacgagaa attccgcggc
       850        860        870        880        890        900
gtacgtcttt atgtcagcga aaaccagctg aaaatcaccg ccaacaaccc ggaacaggaa
       910        920        930        940        950        960
gaagcggaag agatcctcga cgttacctat agcggtgcgg agatggaaat cggcttcaac
       970        980        990       1000       1010       1020
gtcagttatg tgctggatgt tctgaacgcg ctgaaatgcg aaaacgtccg catgatgctg
      1030       1040       1050       1060       1070       1080
accgattcgg tttccagcgt gcagattgaa gatgcggcca gccagagcgc ggcttatgtt
      1090       1100
gtcatgccaa tgagactgta a   

Last modification date: Oct. 2, 2011