Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: ATP-dependent DNA helicase Rep


DESCRIPTION:
ATP-dependent DNA helicase Rep [EC:3.6.4.12] Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction.

STRUCTURE SIMILARITY:
Belongs to the helicase family. UvrD subfamily.
Contains: 1 uvrD-like helicase ATP-binding domain.
Contains: 1 uvrD-like helicase C-terminal domain.
Pfam: UvrD_C UvrD-helicase AAA_19 UvrD_C_2 AAA_30 Viral_helicase1 Exonuc_V_gamma DEAD
PROSITE: UVRD_HELICASE_ATP_BIND UVRD_HELICASE_CTER


CATALYTIC ACTIVITY:
ATP + H2O = ADP + phosphate.


PROTEIN TYPE(S):
3'-5' DNA helicase


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MRLNPGQQQA VEFVTGPCLV LAGAGSGKTR VITNKIAHLI RGCGYQARHI AAVTFTNKAA
        70         80         90        100        110        120
REMKERVGQT LGRKEARGLM ISTFHTLGLD IIKREYAALG MKANFSLFDD TDQLALLKEL
       130        140        150        160        170        180
TEGLIEDDKV LLQQLISTIS NWKNDLKTPS QAAASAIGER DRIFAHCYGL YDAHLKACNV
       190        200        210        220        230        240
LDFDDLILLP TLLLQRNEEV RKRWQNKIRY LLVDEYQDTN TSQYELVKLL VGSRARFTVV
       250        260        270        280        290        300
GDDDQSIYSW RGARPQNLVL LSQDFPALKV IKLEQNYRSS GRILKAANIL IANNPHVFEK
       310        320        330        340        350        360
RLFSELGYGA ELKVLSANNE EHEAERVTGE LIAHHFVNKT QYKDYAILYR GNHQSRVFEK
       370        380        390        400        410        420
FLMQNRIPYK ISGGTSFFSR PEIKDLLAYL RVLTNPDDDS AFLRIVNTPK REIGPATLKK
       430        440        450        460        470        480
LGEWAMTRNK SMFTASFDMG LSQTLSGRGY EALTRFTHWL AEIQRLAERE PIAAVRDLIH
       490        500        510        520        530        540
GMDYESWLYE TSPSPKAAEM RMKNVNQLFS WMTEMLEGSE LDEPMTLTQV VTRFTLRDMM
       550        560        570        580        590        600
ERGESEEELD QVQLMTLHAS KGLEFPYVYM VGMEEGFLPH QSSIDEDNID EERRLAYVGI
       610        620        630        640        650        660
TRAQKELTFT LCKERRQYGE LVRPEPSRFL LELPQDDLIW EQERKVVSAE ERMQKGQSHL
       670
ANLKAMMAAK RGK    

Encoded by rep gene

FULL NAME: DNA helicase and single-stranded DNA-dependent ATPase


OTHER NAME(S):
ECK3770
JW5604


DESCRIPTION:
Rep is an ATP-dependent helicase that is required for genomic replication in Escherichia coli and some bacteriophages . [More information is available at EcoCyc: EG10837].


Nucleic acid sequence

        10         20         30         40         50         60
atgcgtctaa accccggcca acaacaagct gtcgaattcg ttaccggccc ctgcctggtg
        70         80         90        100        110        120
ctggcgggcg cgggttccgg taaaactcgt gttatcacca ataaaatcgc ccatctgatc
       130        140        150        160        170        180
cgcggttgcg gttatcaggc gcggcacatt gcggcggtga cctttactaa taaagcagcg
       190        200        210        220        230        240
cgcgagatga aagagcgtgt agggcagacg ctggggcgca aagaggcgcg tgggctgatg
       250        260        270        280        290        300
atctccactt tccatacgtt ggggctggat atcatcaaac gcgagtatgc ggcgcttggg
       310        320        330        340        350        360
atgaaagcga acttctcgtt gtttgacgat accgatcagc ttgctttgct taaagagttg
       370        380        390        400        410        420
accgaggggc tgattgaaga tgacaaagtt ctcctgcaac aactgatttc gaccatctct
       430        440        450        460        470        480
aactggaaga atgatctcaa aacaccgtcc caggcggcag caagtgcgat tggcgagcgg
       490        500        510        520        530        540
gaccgtattt ttgcccattg ttatgggctg tatgatgcac acctgaaagc ctgtaacgtt
       550        560        570        580        590        600
ctcgacttcg atgatctgat tttattgccg acgttgctgc tgcaacgcaa tgaagaagtc
       610        620        630        640        650        660
cgcaagcgct ggcagaacaa aattcgctat ctgctggtgg atgagtatca ggacaccaac
       670        680        690        700        710        720
accagccagt atgagctggt gaaactgctg gtgggcagcc gcgcgcgctt taccgtggtg
       730        740        750        760        770        780
ggtgacgatg accagtcgat ctactcctgg cgcggtgcac gtccgcaaaa cctggtgctg
       790        800        810        820        830        840
ctgagtcagg attttccggc gctgaaggtg attaagcttg agcagaacta tcgctcttcc
       850        860        870        880        890        900
gggcgtattc tgaaagcggc gaacatcctg atcgccaata acccgcacgt ctttgaaaag
       910        920        930        940        950        960
cgtctgttct ccgaactggg ttatggcgcg gagctaaaag tattaagcgc gaataacgaa
       970        980        990       1000       1010       1020
gaacatgagg ctgagcgcgt tactggcgag ctgatcgccc atcacttcgt caataaaacg
      1030       1040       1050       1060       1070       1080
cagtacaaag attacgccat tctttatcgc ggtaaccatc agtcgcgggt gtttgaaaaa
      1090       1100       1110       1120       1130       1140
ttcctgatgc aaaaccgcat cccgtacaaa atatctggtg gtacgtcgtt tttctctcgt
      1150       1160       1170       1180       1190       1200
cctgaaatca aggacttgct ggcttatctg cgcgtgctga ctaacccgga cgatgacagc
      1210       1220       1230       1240       1250       1260
gcatttctgc gtatcgttaa cacgccgaag cgagagattg gcccggctac gctgaaaaag
      1270       1280       1290       1300       1310       1320
ctgggtgagt gggcgatgac gcgcaataaa agcatgttta ccgccagctt tgatatgggc
      1330       1340       1350       1360       1370       1380
ctgagtcaga cgcttagcgg acgtggttat gaagcattga cccgcttcac tcactggttg
      1390       1400       1410       1420       1430       1440
gcagaaatcc agcgtctggc ggagcgggag ccgattgccg cggtgcgtga tctgatccat
      1450       1460       1470       1480       1490       1500
ggcatggatt atgaatcctg gctgtacgaa acatcgccca gcccgaaagc cgccgaaatg
      1510       1520       1530       1540       1550       1560
cgcatgaaga acgtcaacca actgtttagc tggatgacgg agatgctgga aggcagtgaa
      1570       1580       1590       1600       1610       1620
ctggatgagc cgatgacgct cacccaggtg gtgacgcgct ttactttgcg cgacatgatg
      1630       1640       1650       1660       1670       1680
gagcgtggtg agagtgaaga agagctggat caggtgcaac tgatgactct ccacgcgtcg
      1690       1700       1710       1720       1730       1740
aaagggctgg agtttcctta tgtctacatg gtcggtatgg aagaagggtt tttgccgcac
      1750       1760       1770       1780       1790       1800
cagagcagca tcgatgaaga taatatcgat gaggagcggc ggctggccta tgtcggcatt
      1810       1820       1830       1840       1850       1860
acccgcgccc agaaggaatt gacctttacg ctgtgtaaag aacgccgtca gtacggcgaa
      1870       1880       1890       1900       1910       1920
ctggtgcgcc cggagccgag ccgctttttg ctggagctgc cgcaggatga tctgatttgg
      1930       1940       1950       1960       1970       1980
gaacaggagc gcaaagtggt cagcgccgaa gaacggatgc agaaagggca aagccatctg
      1990       2000       2010       2020
gcgaatctga aagcgatgat ggcggcaaaa cgagggaaat aa 

Last modification date: Dec. 31, 2011