Homo sapiens L. (human) [HSA]

FULL NAME: Probable ATP-dependent RNA helicase DDX11


DESCRIPTION:
DNA helicase involved in cellular proliferation.
Possesses DNA-dependent ATPase and helicase activities. This helicase translocates on single-stranded DNA in the 5' to 3' direction in the presence of ATP and, to a lesser extent, dATP. Its unwinding activity requires a 5'-single-stranded region for helicase loading, since flush-ended duplex structures do not support unwinding. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended to 500 bp by RPA or the cohesion establishment factor, the Ctf18-RFC (replication factor C) complex activities. Stimulates the flap endonuclease activity of FEN1. Required for normal sister chromatid cohesion. Required for recruitement of bovine papillomavirus type 1 regulatory protein E2 to mitotic chrmosomes and for viral genome maintenance. Required for maintaining the chromosome segregation and is essential for embryonic development and the prevention of aneuploidy. May function during either S, G2, or M phase of the cell cycle. Binds to both single- and double-stranded DNA.

STRUCTURE SIMILARITY:
Contains 1 helicase ATP-binding domain.
Belongs to the DEAD box helicase family. DEAH subfamily. DDX11/CHL1 sub-subfamily.


CATALYTIC ACTIVITY:
ATP + H(2)O = ADP + phosphate.


ENZYME REGULATION:
Helicase shows maximal activity with magnesium ions at low concentrations (0.5-1mM) whereas is markedly inhibited at higher levels (5 mM and above). Stimulated by 25-50 mM potassium acetate, stimulated to a lesser extent by 25 mM of ammonium acetate, and markedly inhibited by sodium acetate. The ATPase activity is stimulated by high salt levels (up to a 0.1 M) and potassium salts (glutamate, chloride or acetate) are more effective than the corresponding sodium salts.


PROTEIN TYPE(S):
5'-3' DNA helicase


RELATED PATHWAY(S):
DNA replication


RELATED DISEASE(S):
Warsaw breakage syndrome (WBRS)


Amino acids sequence

        10         20         30         40         50         60
MANETQKVGA IHFPFPFTPY SIQEDFMAEL YRVLEAGKIG IFESPTGTGK SLSLICGALS
        70         80         90        100        110        120
WLRDFEQKKR EEEARLLETG TGPLHDEKDE SLCLSSSCEG AAGTPRPAGE PAWVTQFVQK
       130        140        150        160        170        180
KEERDLVDRL KAEQARRKQR EERLQQLQHR VQLKYAAKRL RQEEEERENL LRLSREMLET
       190        200        210        220        230        240
GPEAERLEQL ESGEEELVLA EYESDEEKKV ASRVDEDEDD LEEEHITKIY YCSRTHSQLA
       250        260        270        280        290        300
QFVHEVKKSP FGKDVRLVSL GSRQNLCVNE DVKSLGSVQL INDRCVDMQR SRHEKKKGAE
       310        320        330        340        350        360
EEKPKRRRQE KQAACPFYNH EQMGLLRDEA LAEVKDMEQL LALGKEARAC PYYGSRLAIP
       370        380        390        400        410        420
AAQLVVLPYQ MLLHAATRQA AGIRLQDQVV IIDEAHNLID TITGMHSVEV SGSQLCQAHS
       430        440        450        460        470        480
QLLQYVERYG KRLKAKNLMY LKQILYLLEK FVAVLGGNIK QNPNTQSLSQ TGTELKTIND
       490        500        510        520        530        540
FLFQSQIDNI NLFKVQRYCE KSMISRKLFG FTERYGAVFS SREQPKLAGF QQFLQSLQPR
       550        560        570        580        590        600
TTEALAAPAD ESQASTLRPA SPLMHIQGFL AALTTANQDG RVILSRQGSL SQSTLKFLLL
       610        620        630        640        650        660
NPAVHFAQVV KECRAVVIAG GTMQPVSDFR QQLLACAGVE AERVVEFSCG HVIPPDNILP
       670        680        690        700        710        720
LVICSGISNQ PLEFTFQKRE LPQMIFQEPK SAHQVEQVLL AYSRCIQACG QERGQVTGAL
       730        740        750        760        770        780
LLSVVGGKMS EGINFSDNLG RCVVMVGMPF PNIRSAELQE KMAYLDQTLP RAPGQAPPGK
       790        800        810        820        830        840
ALVENLCMKA VNQSIGRAIR HQKDFASVVL LDQRYARPPV LAKLPAWIRA RVEVKATFGP
       850
AIAAVQKFHR EKSASS    

Encoded by LOC652522 gene

FULL NAME: Werner syndrome ATP-dependent helicase-like


Nucleic acid sequence

        10         20         30         40         50         60
atggctaatg aaacacagaa ggttggtgcc atccattttc cttttccctt cacaccctat
        70         80         90        100        110        120
tccatccagg aagacttcat ggcagagctg taccgggttt tggaggctgg caagattggg
       130        140        150        160        170        180
atatttgaga gtccaactgg cactgggaag tccttaagtc ttatttgtgg ggccctctct
       190        200        210        220        230        240
tggctccgtg actttgaaca gaagaagcgt gaagaagagg cacgactcct tgaaactgga
       250        260        270        280        290        300
actggcccct tacatgatga gaaagatgaa tccctgtgtc tgtcttcttc ctgcgaaggg
       310        320        330        340        350        360
gctgcaggca ccccgaggcc tgctggagaa ccggcctggg ttactcagtt tgtgcagaag
       370        380        390        400        410        420
aaagaagaga gggacctggt ggaccgacta aaggcggagc aggccaggag gaagcagcga
       430        440        450        460        470        480
gaagaacgcc tgcagcagct gcagcacagg gtgcagctca agtatgcagc caagcgcctg
       490        500        510        520        530        540
aggcaggaag aagaagaaag agagaatctc ctccgcctca gcagggagat gctagagaca
       550        560        570        580        590        600
ggcccggagg ctgagcggct ggagcagctg gagtctgggg aggaggagct ggtcctcgcc
       610        620        630        640        650        660
gaatacgaga gtgatgagga gaaaaaggtg gcgagcagag tggatgagga tgaggatgac
       670        680        690        700        710        720
ctggaggaag aacacataac taagatttat tactgtagtc ggacacactc ccagctggcc
       730        740        750        760        770        780
cagtttgtgc atgaggtgaa gaagagcccc tttggcaagg atgttcggct ggtctccctt
       790        800        810        820        830        840
ggctcccggc agaacctttg tgtaaatgaa gacgtgaaaa gcctaggttc tgtgcagctt
       850        860        870        880        890        900
atcaacgacc gctgtgtgga catgcagaga agcaggcacg agaagaagaa aggagctgag
       910        920        930        940        950        960
gaggagaagc caaagaggag gaggcaggag aagcaggcag cctgcccctt ctacaaccac
       970        980        990       1000       1010       1020
gagcagatgg gccttctccg ggatgaggcc ctggcagagg tgaaggacat ggagcagctg
      1030       1040       1050       1060       1070       1080
ctggcccttg ggaaggaggc ccgggcctgt ccctattacg ggagccgcct tgccatccct
      1090       1100       1110       1120       1130       1140
gcagcccagc tggtggtgct gccctatcag atgctgctgc atgcggccac tcggcaggcc
      1150       1160       1170       1180       1190       1200
gcgggcatcc ggctgcagga ccaggtggtg atcatcgacg aggcgcacaa cctgatcgac
      1210       1220       1230       1240       1250       1260
accatcacgg gcatgcacag cgtggaggtc agcggctccc agctctgcca ggcccattcc
      1270       1280       1290       1300       1310       1320
cagctgctgc agtacgtgga gcgatacggg aagcgtttga aggccaagaa cctgatgtac
      1330       1340       1350       1360       1370       1380
ctgaagcaga tcctgtattt gctggagaaa ttcgtggctg tgctaggggg gaacattaag
      1390       1400       1410       1420       1430       1440
caaaatccca atacacagag tctgtcacag acagggacgg agctgaagac catcaacgac
      1450       1460       1470       1480       1490       1500
tttctcttcc agagccagat cgacaacatc aacctgttca aggtgcagcg atactgtgag
      1510       1520       1530       1540       1550       1560
aagagcatga tcagcagaaa gctctttgga ttcactgaac ggtacggagc agtgttctca
      1570       1580       1590       1600       1610       1620
tcccgggagc agcccaaact ggctgggttt cagcaattcc tgcagagcct gcagcccagg
      1630       1640       1650       1660       1670       1680
acgactgaag ctcttgcagc ccctgcagac gagagtcagg ccagcaccct gcgaccagct
      1690       1700       1710       1720       1730       1740
tctccactga tgcacatcca aggcttcctg gcagctctca ctacggccaa ccaggacggc
      1750       1760       1770       1780       1790       1800
agggtcatcc tgagccgcca aggcagcctc agtcagagca ccctgaagtt tttgctcctg
      1810       1820       1830       1840       1850       1860
aatccagctg tgcactttgc ccaagtggtg aaggaatgcc gggcagtggt cattgcgggg
      1870       1880       1890       1900       1910       1920
ggtaccatgc agccggtgtc tgacttccgg cagcagctgc tggcctgtgc cggggtggaa
      1930       1940       1950       1960       1970       1980
gctgagcgcg tggtggagtt ttcctgtggt cacgtgatcc ctccagacaa catcctgccc
      1990       2000       2010       2020       2030       2040
ctcgtcatct gcagcgggat ctccaaccag ccgctggaat tcacgttcca gaaaagagag
      2050       2060       2070       2080       2090       2100
ctgcctcaga tgatattcca ggaacctaag agcgcacacc aggtggagca ggtgctgctg
      2110       2120       2130       2140       2150       2160
gcatattcca ggtgcatcca ggcctgtggc caggagagag gccaggtgac aggggccctg
      2170       2180       2190       2200       2210       2220
ctcctctctg tggttggagg aaagatgagt gaagggatca acttctctga caacctaggc
      2230       2240       2250       2260       2270       2280
cggtgtgtgg tgatggtggg catgcccttc cccaacatca ggtctgcaga gctgcaggag
      2290       2300       2310       2320       2330       2340
aagatggcct acttggatca aaccctcccc agagcccccg gccaggcacc cccagggaag
      2350       2360       2370       2380       2390       2400
gctctggtgg agaacctgtg catgaaggcc gtcaaccagt ccataggcag ggccatcagg
      2410       2420       2430       2440       2450       2460
caccagaagg attttgccag cgtagtgctc ctggaccagc gatatgcccg gccccctgtc
      2470       2480       2490       2500       2510       2520
ctggccaagc tgccggcctg gatccgagcc cgtgtggagg tcaaagctac ctttggcccc
      2530       2540       2550       2560       2570
gccattgctg ctgtgcagaa gtttcaccgg gagaagtcgg cctcttcctg a

Last modification date: Oct. 8, 2011