Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: Transcription initiation factor TFIID subunit 14


DESCRIPTION:
Functions as a component of the DNA-binding general transcription factor complex TFIID, the RNA polymerase II associated general transcription factor complex TFIIF, and the chromatin-remodeling complex SWI/SNF. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. TFIIF is essential for the initiation of transcription by RNA polymerase II. TFIIF functions include the recruitment of RNA polymerase II to the promoter bound DNA-TBP-TFIIB complex, decreasing the affinity of RNA polymerase II for non-specific DNA, allowing for the subsequent recruitment of TFIIE and TFIIH, and facilitating RNA polymerase II elongation. The TAF14 subunit has stimulatory activity. Component of the SWI/SNF complex, an ATP-dependent chromatin-remodeling complex, is required for the positive and negative regulation of gene expression of a large number of genes. It changes chromatin structure by altering DNA-histone contacts within a nucleosome, leading eventually to a change in nucleosome position, thus facilitating or repressing binding of gene-specific transcription factors. Component of the histone acetyltransferase NuA3 complex, that acetylates Lys-14 of histone H3. Recruitment of NuA3 to nucleosomes requires methylated histone H3. In conjunction with the FACT complex, NuA3 may be involved in transcriptional regulation.

MISCELLANEOUS:
TAF14 is the only non-essential TAF.
There is no homolog of TAF14 present in the TFIIF complexes of higher eukaryotes.
Present with 3100 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Contains 1 YEATS domain.
Belongs to the TAF14 family.


RELATED PATHWAY(S):
transcription factors


Amino acids sequence

        10         20         30         40         50         60
MVATVKRTIR IKTQQHILPE VPPVENFPVR QWSIEIVLLD DEGKEIPATI FDKVIYHLHP
        70         80         90        100        110        120
TFANPNRTFT DPPFRIEEQG WGGFPLDISV FLLEKAGERK IPHDLNFLQE SYEVEHVIQI
       130        140        150        160        170        180
PLNKPLLTEE LAKSGSTEET TANTGTIGKR RTTTNTTAEP KAKRAKTGSA STVKGSVDLE
       190        200        210        220        230        240
KLAFGLTKLN EDDLVGVVQM VTDNKTPEMN VTNNVEEGEF IIDLYSLPEG LLKSLWDYVK

KNTE     

Encoded by TAF14 gene


OTHER NAME(S):
ANC1
SWP29
TAF30
TFG3


Nucleic acid sequence

        10         20         30         40         50         60
atggtagcta cagtaaaaag aaccatccgt ataaaaaccc aacaacacat tctgccagaa
        70         80         90        100        110        120
gtgccaccag tagagaattt tcctgttcgt caatggagta tagaaattgt attattggat
       130        140        150        160        170        180
gatgaaggaa aagaaattcc cgctacaatt tttgacaaag ttatttacca cttgcaccca
       190        200        210        220        230        240
actttcgcca atcctaacag gacatttact gacccaccat ttagaattga ggaacaaggt
       250        260        270        280        290        300
tggggtggat ttcctttaga tataagcgtt ttcttgttgg aaaaagcagg tgagcggaaa
       310        320        330        340        350        360
ataccacacg atttgaactt tctacaagag agttatgaag tggagcacgt tatccagatt
       370        380        390        400        410        420
cctctcaata aaccactttt aacagaagaa cttgcgaaaa gtggatctac tgaagaaacg
       430        440        450        460        470        480
acggccaata caggcaccat tgggaaaaga aggactacca cgaacacaac tgcagaacca
       490        500        510        520        530        540
aaggcgaaga gagctaagac gggcagtgca tctaccgtga aagggagcgt ggacctagaa
       550        560        570        580        590        600
aaattagcgt tcggattgac taaactaaat gaagatgacc tggttggtgt tgttcaaatg
       610        620        630        640        650        660
gttaccgaca ataaaacacc agaaatgaac gtgacgaata atgttgaaga gggtgaattt
       670        680        690        700        710        720
ataattgact tgtatagttt acctgaggga ttattgaaaa gtctatggga ctacgttaag
       730
aaaaataccg agtaa    

Last modification date: Oct. 2, 2011