Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: Transcription initiation factor TFIID subunit 6


DESCRIPTION:
Functions as a component of the DNA-binding general transcription factor complex TFIID and the regulatory transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and SLIK. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus.

MISCELLANEOUS:
Present with 6506 (+/-1290) molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Belongs to the TAF6 family.
Contains 1 histone-fold domain.


RELATED PATHWAY(S):
transcription factors


Amino acids sequence

        10         20         30         40         50         60
MSTQQQSYTI WSPQDTVKDV AESLGLENIN DDVLKALAMD VEYRILEIIE QAVKFKRHSK
        70         80         90        100        110        120
RDVLTTDDVS KALRVLNVEP LYGYYDGSEV NKAVSFSKVN TSGGQSVYYL DEEEVDFDRL
       130        140        150        160        170        180
INEPLPQVPR LPTFTTHWLA VEGVQPAIIQ NPNLNDIRVS QPPFIRGAIV TALNDNSLQT
       190        200        210        220        230        240
PVTSTTASAS VTDTGASQHL SNVKPGQNTE VKPLVKHVLS KELQIYFNKV ISTLTAKSQA
       250        260        270        280        290        300
DEAAQHMKQA ALTSLRTDSG LHQLVPYFIQ FIAEQITQNL SDLQLLTTIL EMIYSLLSNT
       310        320        330        340        350        360
SIFLDPYIHS LMPSILTLLL AKKLGGSPKD DSPQEIHEFL ERTNALRDFA ASLLDYVLKK
       370        380        390        400        410        420
FPQAYKSLKP RVTRTLLKTF LDINRVFGTY YGCLKGVSVL EGESIRFFLG NLNNWARLVF
       430        440        450        460        470        480
NESGITLDNI EEHLNDDSNP TRTKFTKEET QILVDTVISA LLVLKKDLPD LYEGKGEKVT
       490        500        510
DEDKEKLLER CGVTIGFHIL KRDDAKELIS AIFFGE  

Encoded by TAF6 gene


OTHER NAME(S):
TAF60


Nucleic acid sequence

        10         20         30         40         50         60
atgtctacac agcagcaatc ttacacaatt tggtctccac aagatactgt taaagatgtc
        70         80         90        100        110        120
gctgagtcgc ttgggttgga aaatatcaac gatgacgtgt tgaaagcact tgctatggac
       130        140        150        160        170        180
gttgaatacc gtattctaga gattattgaa caggcagtca agtttaaaag acactctaaa
       190        200        210        220        230        240
agagatgttt taactacaga cgatgtatcg aaggccctgc gtgttctgaa tgtggaacct
       250        260        270        280        290        300
ttatatggat attatgatgg ctctgaagtt aataaagctg tatcatttag taaggttaac
       310        320        330        340        350        360
acaagtggag gacagtcggt ttactacctg gatgaggaag aagtagattt tgatagatta
       370        380        390        400        410        420
ataaatgagc cattaccgca agtgcctcgt ctaccaactt ttactacaca ttggctagca
       430        440        450        460        470        480
gttgaagggg ttcaacctgc cataatccaa aatccaaatt tgaatgatat aagggtatcc
       490        500        510        520        530        540
caaccaccat ttattagagg tgctattgtc actgctttga atgataacag cctccaaaca
       550        560        570        580        590        600
cctgtaacgt cgacgacagc aagtgcttct gtgacggata caggcgcctc tcaacacctg
       610        620        630        640        650        660
tctaatgtta aaccaggaca gaatactgaa gtcaaacctt tagtgaaaca cgttttatcc
       670        680        690        700        710        720
aaagaattac agatttattt caataaggtc atttcaacct tgacagcgaa gagtcaagct
       730        740        750        760        770        780
gatgaagctg cacagcatat gaaacaagca gctttgactt cactacgaac tgatagtggc
       790        800        810        820        830        840
ctgcaccaac tggttccata ttttattcaa tttattgccg aacaaatcac acaaaatctt
       850        860        870        880        890        900
tccgatttac aattgctaac aacaattttg gaaatgattt actctttact aagcaatact
       910        920        930        940        950        960
tctattttcc tggaccctta tattcattcg ttaatgcctt ccattttaac cttgctatta
       970        980        990       1000       1010       1020
gcaaaaaaac ttggtggatc accaaaagat gattcgccgc aagaaattca tgaattttta
      1030       1040       1050       1060       1070       1080
gaaagaacga atgcactacg tgattttgcc gcatctttgt tggactatgt attgaaaaaa
      1090       1100       1110       1120       1130       1140
tttcctcaag catacaaatc cctgaagcca agagtaacta ggacactgct aaagacattt
      1150       1160       1170       1180       1190       1200
ttggacataa accgtgtttt tgggacttac tatgggtgct taaagggtgt atcggtacta
      1210       1220       1230       1240       1250       1260
gaaggtgaat ccatcagatt cttcttagga aacctaaata attgggcgcg cttggtattc
      1270       1280       1290       1300       1310       1320
aatgaaagcg gaataactct agacaatata gaggaacatt tgaacgatga ctccaatcca
      1330       1340       1350       1360       1370       1380
actaggacca aatttactaa agaagaaact caaatcttgg ttgatactgt aattagtgca
      1390       1400       1410       1420       1430       1440
ttgttagttc taaaaaagga tttaccagat ttgtacgagg gtaaaggtga aaaagttacg
      1450       1460       1470       1480       1490       1500
gatgaggata aggaaaaatt gctagagagg tgtggtgtca ctattggatt tcacattttg
      1510       1520       1530       1540       1550
aaaagagacg acgcaaaaga attaattagt gcgatatttt ttggcgaata g

Last modification date: Oct. 2, 2011