Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: Transcription initiation factor TFIID subunit 5


DESCRIPTION:
Functions as a component of the DNA-binding general transcription factor complex TFIID and the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and SLIK. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus.

MISCELLANEOUS:
Present with 14834 (+/-203) molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Belongs to the WD repeat TAF5 family.
Contains 6 WD repeats.
Contains 1 LisH domain.


RELATED PATHWAY(S):
transcription factors


Amino acids sequence

        10         20         30         40         50         60
MSQKQSTNQN QNGTHQPQPV KNQRTNNAAG ANSGQQPQQQ SQGQSQQQGR SNGPFSASDL
        70         80         90        100        110        120
NRIVLEYLNK KGYHRTEAML RAESGRTLTP QNKQSPANTK TGKFPEQSSI PPNPGKTAKP
       130        140        150        160        170        180
ISNPTNLSSK RDAEGGIVSS GRLEGLNAPE NYIRAYSMLK NWVDSSLEIY KPELSYIMYP
       190        200        210        220        230        240
IFIYLFLNLV AKNPVYARRF FDRFSPDFKD FHGSEINRLF SVNSIDHIKE NEVASAFQSH
       250        260        270        280        290        300
KYRITMSKTT LNLLLYFLNE NESIGGSLII SVINQHLDPN IVESVTAREK LADGIKVLSD
       310        320        330        340        350        360
SENGNGKQNL EMNSVPVKLG PFPKDEEFVK EIETELKIKD DQEKQLNQQT AGDNYSGANN
       370        380        390        400        410        420
RTLLQEYKAM NNEKFKDNTG DDDKDKIKDK IAKDEEKKES ELKVDGEKKD SNLSSPARDI
       430        440        450        460        470        480
LPLPPKTALD LKLEIQKVKE SRDAIKLDNL QLALPSVCMY TFQNTNKDMS CLDFSDDCRI
       490        500        510        520        530        540
AAAGFQDSYI KIWSLDGSSL NNPNIALNNN DKDEDPTCKT LVGHSGTVYS TSFSPDNKYL
       550        560        570        580        590        600
LSGSEDKTVR LWSMDTHTAL VSYKGHNHPV WDVSFSPLGH YFATASHDQT ARLWSCDHIY
       610        620        630        640        650        660
PLRIFAGHLN DVDCVSFHPN GCYVFTGSSD KTCRMWDVST GDSVRLFLGH TAPVISIAVC
       670        680        690        700        710        720
PDGRWLSTGS EDGIINVWDI GTGKRLKQMR GHGKNAIYSL SYSKEGNVLI SGGADHTVRV
       730        740        750        760        770        780
WDLKKATTEP SAEPDEPFIG YLGDVTASIN QDIKEYGRRR TVIPTSDLVA SFYTKKTPVF
       790
KVKFSRSNLA LAGGAFRP    

Encoded by TAF5 gene


OTHER NAME(S):
TAF90


Nucleic acid sequence

        10         20         30         40         50         60
atgtcacaga agcaaagtac gaatcagaat caaaatggca cgcatcagcc acaaccagta
        70         80         90        100        110        120
aagaatcaac gaactaataa tgctgctggc gccaattccg gccagcagcc acagcaacag
       130        140        150        160        170        180
agtcaaggcc agtctcaaca gcaggggagg tccaatggtc cgttttcggc gtcagacttg
       190        200        210        220        230        240
aatagaattg tcctagagta cctgaataaa aagggatacc accgtacaga ggccatgctg
       250        260        270        280        290        300
agagccgaga gtggacgtac actaacacct caaaacaaac aatcaccagc aaatactaaa
       310        320        330        340        350        360
acaggtaaat ttcctgaaca atcatcaatt cctccgaacc ccgggaagac agctaaaccc
       370        380        390        400        410        420
ataagcaatc caactaattt gtcatccaag agagatgcag aaggtggtat agtatctagc
       430        440        450        460        470        480
gggcgactag aagggctcaa cgcacctgaa aattatatac gtgcatattc aatgttgaaa
       490        500        510        520        530        540
aactgggtag attcgtcgct ggaaatttac aagccggaac taagctacat catgtaccca
       550        560        570        580        590        600
atattcattt atttattttt aaatttggtg gccaaaaatc ctgtttacgc ccgtcgtttc
       610        620        630        640        650        660
tttgatagat tttctcccga ttttaaggac ttccatggct cagaaattaa tagacttttc
       670        680        690        700        710        720
agtgtcaatt ctatagatca catcaaggaa aatgaggtcg caagtgcttt ccaatctcac
       730        740        750        760        770        780
aagtacagaa ttactatgtc taaaaccact ttaaatcttc tcttatattt tttgaatgaa
       790        800        810        820        830        840
aatgagagta ttggaggttc attaataatt agtgtaataa atcagcacct agatccaaat
       850        860        870        880        890        900
attgtagaat cagtaaccgc aagggaaaaa ttggctgatg gaataaaagt tctcagtgat
       910        920        930        940        950        960
agtgaaaacg gaaatggtaa acaaaattta gaaatgaatt ctgttcctgt caagctggga
       970        980        990       1000       1010       1020
cctttcccaa aagacgagga gtttgttaag gagattgaaa ctgaattgaa aattaaggat
      1030       1040       1050       1060       1070       1080
gatcaagaga aacaactaaa tcaacaaact gcaggtgaca attacagtgg agccaacaat
      1090       1100       1110       1120       1130       1140
agaaccttat tgcaagaata taaagctatg aataacgaaa aatttaagga taatactggc
      1150       1160       1170       1180       1190       1200
gacgatgata aagacaaaat taaagataaa atcgccaaag atgaggaaaa aaaagagagc
      1210       1220       1230       1240       1250       1260
gaattgaagg tggatggtga aaaaaaagac agcaatttat cttcgccagc acgtgatatt
      1270       1280       1290       1300       1310       1320
ttacctttac caccaaagac ggcactagat ttgaagttgg agattcagaa ggtcaaggaa
      1330       1340       1350       1360       1370       1380
tctcgtgatg ccatcaaact agataattta cagttggcct taccaagcgt gtgcatgtat
      1390       1400       1410       1420       1430       1440
acattccaaa acacaaataa agacatgtca tgtttagatt tcagtgatga ttgtaggata
      1450       1460       1470       1480       1490       1500
gcggctgctg gctttcaaga cagttatatc aagatctggt cactcgacgg ctcatcactt
      1510       1520       1530       1540       1550       1560
aataatccta acatcgcgtt aaataacaac gacaaggatg aagacccaac gtgtaaaact
      1570       1580       1590       1600       1610       1620
ttggtggggc acagtggtac agtatattca acaagtttca gtccggataa taagtattta
      1630       1640       1650       1660       1670       1680
ctttctggtt ctgaagataa aaccgtcagg ctatggtcca tggatactca caccgcgcta
      1690       1700       1710       1720       1730       1740
gtaagctata agggccataa tcaccctgtg tgggatgtta gtttctcccc gctaggccat
      1750       1760       1770       1780       1790       1800
tatttcgcca ctgcatcgca cgaccaaacc gcaaggttat ggtcatgcga tcacatatat
      1810       1820       1830       1840       1850       1860
ccattaagaa tatttgcagg acatttaaat gatgttgatt gtgtgtcatt tcatccgaac
      1870       1880       1890       1900       1910       1920
ggttgctatg tgtttacggg atccagcgat aagacgtgtc gaatgtggga cgtttctaca
      1930       1940       1950       1960       1970       1980
ggcgactcgg ttaggttatt tcttggacat actgcgcccg tcatttctat tgctgtttgt
      1990       2000       2010       2020       2030       2040
ccagatggta ggtggttgtc cactggtagc gaagatggta ttattaatgt atgggatatt
      2050       2060       2070       2080       2090       2100
ggtaccggca aaaggttgaa acaaatgcgt ggccatggta agaatgccat ttattcctta
      2110       2120       2130       2140       2150       2160
tcatatagca aagagggaaa cgtacttatt agtggaggtg ccgatcatac agtccgtgta
      2170       2180       2190       2200       2210       2220
tgggatttga aaaaggccac tactgagcca agcgcagaac cagatgagcc attcattgga
      2230       2240       2250       2260       2270       2280
tacttgggtg acgttactgc atctataaac caagatataa aagaatatgg tcgcagaaga
      2290       2300       2310       2320       2330       2340
acagtaatac ctacaagcga tttagtagcg tcgttctaca ccaaaaagac gcctgttttt
      2350       2360       2370       2380       2390
aaggtcaagt tcagcagaag caatttagcc cttgcaggag gtgctttcag gccataa

Last modification date: Oct. 2, 2011