Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: Transcription initiation factor TFIID subunit 12


DESCRIPTION:
Functions as a component of the DNA-binding general transcription factor complex TFIID and the transcription regulatory histone acetylation (HAT) complexes SAGA, SALSA and SLIK. Binding of TFIID to a promoter (with or without TATA element) is the initial step in preinitiation complex (PIC) formation. TFIID plays a key role in the regulation of gene expression by RNA polymerase II through different activities such as transcription activator interaction, core promoter recognition and selectivity, TFIIA and TFIIB interaction, chromatin modification (histone acetylation by TAF1), facilitation of DNA opening and initiation of transcription. SAGA is involved in RNA polymerase II-dependent transcriptional regulation of approximately 10% of yeast genes. At the promoters, SAGA is required for recruitment of the basal transcription machinery. It influences RNA polymerase II transcriptional activity through different activities such as TBP interaction (SPT3, SPT8 and SPT20) and promoter selectivity, interaction with transcription activators (GCN5, ADA2, ADA3 and TRA1), and chromatin modification through histone acetylation (GCN5) and deubiquitination (UBP8). SAGA acetylates nucleosomal histone H3 to some extent (to form H3K9ac, H3K14ac, H3K18ac and H3K23ac). SAGA interacts with DNA via upstream activating sequences (UASs). SALSA, an altered form of SAGA, may be involved in positive transcriptional regulation. SLIK is proposed to have partly overlapping functions with SAGA. It preferentially acetylates methylated histone H3, at least after activation at the GAL1-10 locus.

MISCELLANEOUS:
Present with 930 (+/-45) molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Contains 1 histone-fold domain.
Belongs to the TAF12 family.


RELATED PATHWAY(S):
transcription factors


Amino acids sequence

        10         20         30         40         50         60
MSSNPENSGV NANNNTGTGN ADAITGAQQN MVLQPRQLQE MAAKFRTLLT EARNVGETTP
        70         80         90        100        110        120
RGKELMFQAA KIKQVYDALT LNRRRQQAAQ AYNNTSNSNS SNPASIPTEN VPNSSQQQQQ
       130        140        150        160        170        180
QQQQTRNNSN KFSNMIKQVL TPEENQEYEK LWQNFQVRHT SIKEKETYLK QNIDRLEQEI
       190        200        210        220        230        240
NKQTDEGPKQ QLQEKKIELL NDWKVLKIEY TKLFNNYQNS KKTFYVECAR HNPALHKFLQ
       250        260        270        280        290        300
ESTQQQRVQQ QRVQQQQQQQ QQQQQQQQQQ QQQQQQRQGQ NQRKISSSNS TEIPSVTGPD
       310        320        330        340        350        360
ALKSQQQQQN TITATNNPRG NVNTSQTEQS KAKVTNVNAT ASMLNNISSS KSAIFKQTEP
       370        380        390        400        410        420
AIPISENIST KTPAPVAYRS NRPTITGGSA MNASALNTPA TTKLPPYEMD TQRVMSKRKL
       430        440        450        460        470        480
RELVKTVGID EGDGETVIDG DVEELLLDLA DDFVTNVTAF SCRLAKHRKS DNLEARDIQL
       490        500        510        520        530
HLERNWNIRI PGYSADEIRS TRKWNPSQNY NQKLQSITSD KVAAAKNNGN NVASLNTKK

Encoded by TAF12 gene


OTHER NAME(S):
TAF61
TAF68


Nucleic acid sequence

        10         20         30         40         50         60
atgtcttcca atccagaaaa ttctggtgtt aatgcgaata ataatacggg cactggtaac
        70         80         90        100        110        120
gctgatgcga tcacaggagc tcagcaaaat atggtactgc aaccgagaca gttgcaagaa
       130        140        150        160        170        180
atggccgcta agttcaggac attactgact gaagcaagaa atgtaggtga aactactcct
       190        200        210        220        230        240
aggggcaagg aattgatgtt ccaagccgca aagatcaaac aggtatatga tgcccttaca
       250        260        270        280        290        300
ctgaatagga gaagacaaca ggctgcgcaa gcctacaata atacttcaaa ttcaaattca
       310        320        330        340        350        360
agcaatccag cttctattcc tactgaaaat gtccctaatt catcacagca acaacaacaa
       370        380        390        400        410        420
caacaacaac agacaagaaa caacagtaac aaatttagca atatgataaa acaggttctc
       430        440        450        460        470        480
accccggaag agaaccaaga atatgaaaag ctatggcaga atttccaagt ccgtcatacg
       490        500        510        520        530        540
agtataaagg agaaagagac ctacttgaaa caaaatattg ataggttaga acaagaaata
       550        560        570        580        590        600
aataaacaga cggacgaagg gcccaagcag cagctacaag aaaagaaaat tgaactgctt
       610        620        630        640        650        660
aacgattgga aggtgctaaa aattgagtat accaagctgt tcaataatta tcaaaacagt
       670        680        690        700        710        720
aaaaaaacat tctatgtaga gtgtgcaaga cacaatccgg ctttacataa attcttgcaa
       730        740        750        760        770        780
gaaagcactc aacagcaacg agtgcagcaa caaagggtac aacaacaaca acaacaacag
       790        800        810        820        830        840
cagcagcagc agcaacagca gcaacagcaa cagcaacagc aacagcaacg ccagggtcaa
       850        860        870        880        890        900
aaccaaagaa agatttctag ttctaattct actgaaatac cctctgtaac cggccctgat
       910        920        930        940        950        960
gcactgaaat cgcagcagca gcagcagaat acaataactg ccaccaataa tcccaggggc
       970        980        990       1000       1010       1020
aatgttaaca cttcacagac tgaacaatcg aaagctaagg taaccaatgt aaatgcaacg
      1030       1040       1050       1060       1070       1080
gcatctatgt tgaataatat aagttcgagc aaatcggcaa tattcaaaca aacagagcct
      1090       1100       1110       1120       1130       1140
gccataccca tatcggaaaa tatatctacc aaaacaccag caccggtagc ttatagatcc
      1150       1160       1170       1180       1190       1200
aacagaccta caataactgg aggttctgct atgaatgcca gtgctttgaa tacaccagca
      1210       1220       1230       1240       1250       1260
acaactaaat taccacccta tgaaatggat actcagagag ttatgtcaaa gcgtaaatta
      1270       1280       1290       1300       1310       1320
agagagttag tgaagactgt cggaattgat gagggtgacg gtgaaactgt cattgacggt
      1330       1340       1350       1360       1370       1380
gatgttgagg aattactatt ggatcttgcc gacgattttg ttactaatgt tacagctttt
      1390       1400       1410       1420       1430       1440
tcttgtagat tggcaaaaca cagaaaatcg gacaatttgg aggcaagaga cattcagtta
      1450       1460       1470       1480       1490       1500
catttggaga gaaattggaa tattaggatt cctggttatt ccgcagacga aataagaagt
      1510       1520       1530       1540       1550       1560
acaagaaaat ggaatccctc tcaaaattat aaccagaaat tgcagagtat cacatcagat
      1570       1580       1590       1600       1610       1620
aaggtagcag ctgcaaaaaa caatggaaac aatgttgcaa gcttgaatac aaaaaaataa

Last modification date: Oct. 2, 2011