Mus musculus L. (mouse) [MMU]

FULL NAME: Werner syndrome ATP-dependent helicase homolog


DESCRIPTION:
Multifunctional enzyme that has both magnesium and ATP-dependent DNA-helicase activity and 3'->5' exonuclease activity towards double-stranded DNA with a 5'-overhang. Has no nuclease activity towards single-stranded DNA or blunt-ended double-stranded DNA. Binds preferentially to DNA substrates containing alternate secondary structures, such as replication forks and Holliday junctions. May play an important role in the dissociation of joint DNA molecules that can arise as products of homologous recombination, at stalled replication forks or during DNA repair. Alleviates stalling of DNA polymerases at the site of DNA lesions. Important for genomic integrity. Plays a role in the formation of DNA replication focal centers; stably associates with foci elements generating binding sites for RP-A (By similarity).

STRUCTURE SIMILARITY:
Contains 1 helicase ATP-binding domain.
Contains 1 HRDC domain.
Contains 1 helicase C-terminal domain.
Contains 1 3'-5' exonuclease domain.
Belongs to the helicase family. RecQ subfamily.


CATALYTIC ACTIVITY:
ATP + H(2)O = ADP + phosphate.


ENZYME REGULATION:
Zinc ions stimulate the exonuclease activity.


POST-TRANSLATIONAL MODIFICATION:
Phosphorylated by PRKDC. Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity).


Amino acids sequence

        10         20         30         40         50         60
METTSLQRKF PEWMSMQSQR CATEEKACVQ KSVLEDNLPF LEFPGSIVYS YEASDCSFLS
        70         80         90        100        110        120
EDISMRLSDG DVVGFDMEWP PIYKPGKRSR VAVIQLCVSE SKCYLFHISS MSVFPQGLKM
       130        140        150        160        170        180
LLENKSIKKA GVGIEGDQWK LLRDFDVKLE SFVELTDVAN EKLKCAETWS LNGLVKHVLG
       190        200        210        220        230        240
KQLLKDKSIR CSNWSNFPLT EDQKLYAATD AYAGLIIYQK LGNLGDTAQV FALNKAEENL
       250        260        270        280        290        300
PLEMKKQLNS ISEEMRDLAN RFPVTCRNLE TLQRVPVILK SISENLCSLR KVICGPTNTE
       310        320        330        340        350        360
TRLKPGSSFN LLSSEDSAAA GEKEKQIGKH STFAKIKEEP WDPELDSLVK QEEVDVFRNQ
       370        380        390        400        410        420
VKQEKGESEN EIEDNLLRED MERTCVIPSI SENELQDLEQ QAKEEKYNDV SHQLSEHLSP
       430        440        450        460        470        480
NDDENDSSYI IESDEDLEME MLKSLENLNS DVVEPTHSTW LEMGTNGRLP PEEEDGHGNE
       490        500        510        520        530        540
AIKEEQEEED HLLPEPNAKQ INCLKTYFGH SSFKPVQWKV IHSVLEERRD NVVVMATGYG
       550        560        570        580        590        600
KSLCFQYPPV YTGKIGIVIS PLISLMEDQV LQLELSNVPA CLLGSAQSKN ILGDVKLGKY
       610        620        630        640        650        660
RVIYITPEFC SGNLDLLQQL DSSIGITLIA VDEAHCISEW GHDFRSSFRM LGSLKTALPL
       670        680        690        700        710        720
VPVIALSATA SSSIREDIIS CLNLKDPQIT CTGFDRPNLY LEVGRKTGNI LQDLKPFLVR
       730        740        750        760        770        780
KASSAWEFEG PTIIYCPSRK MTEQVTAELG KLNLACRTYH AGMKISERKD VHHRFLRDEI
       790        800        810        820        830        840
QCVVATVAFG MGINKADIRK VIHYGAPKEM ESYYQEIGRA GRDGLQSSCH LLWAPADFNT
       850        860        870        880        890        900
SRNLLIEIHD EKFRLYKLKM MVKMEKYLHS SQCRRRIILS HFEDKCLQKA SLDIMGTEKC
       910        920        930        940        950        960
CDNCRPRLNH CLTANNSEDA SQDFGPQAFQ LLSAVDILQE KFGIGIPILF LRGSNSQRLP
       970        980        990       1000       1010       1020
DKYRGHRLFG AGKEQAESWW KTLSHHLIAE GFLVEVPKEN KYIKTCSLTK KGRKWLGEAS
      1030       1040       1050       1060       1070       1080
SQSPPSLLLQ ANEEMFPRKV LLPSSNPVSP ETTQHSSNQN PAGLTTKQSN LERTHSYKVP
      1090       1100       1110       1120       1130       1140
EKVSSGTNIP KKSAVMPSPG TSSSPLEPAI SAQELDARTG LYARLVEARQ KHANKMDVPP
      1150       1160       1170       1180       1190       1200
AILATNKVLL DMAKMRPTTV ENMKQIDGVS EGKAALLAPL LEVIKHFCQV TSVQTDLLSS
      1210       1220       1230       1240       1250       1260
AKPHKEQEKS QEMEKKDCSL PQSVAVTYTL FQEKKMPLHS IAENRLLPLT AAGMHLAQAV
      1270       1280       1290       1300       1310       1320
KAGYPLDMER AGLTPETWKI IMDVIRNPPI NSDMYKVKLI RMLVPENLDT YLIHMAIEIL
      1330       1340       1350       1360       1370       1380
QSGSDSRTQP PCDSSRKRRF PSSAESCESC KESKEAVTET KASSSESKRK LPEWFAKGNV
      1390       1400
PSADTGSSSS MAKTKKKGLF S   

Encoded by Wrn gene

FULL NAME: Werner syndrome homolog (human)


OTHER NAME(S):
AI846146


Nucleic acid sequence

        10         20         30         40         50         60
atggaaacca cttcactaca gcggaaattt ccagaatgga tgtctatgca gagtcaaaga
        70         80         90        100        110        120
tgtgctacag aagaaaaggc ctgcgttcag aagagtgttc ttgaagataa cctcccattc
       130        140        150        160        170        180
ttagaattcc ctggatccat tgtttacagt tatgaagcta gtgattgctc cttcctgtct
       190        200        210        220        230        240
gaagacatta gcatgcgtct gtctgatggc gatgtggtgg gatttgacat ggaatggccg
       250        260        270        280        290        300
cccatataca agccagggaa aagaagcaga gtcgcagtga tccagttgtg tgtgtctgag
       310        320        330        340        350        360
agcaaatgtt acttgtttca catttcttcc atgtcagttt tcccccaggg attaaaaatg
       370        380        390        400        410        420
ttactagaaa acaaatcaat taagaaggca ggggttggga ttgaagggga ccagtggaaa
       430        440        450        460        470        480
cttctgcgtg attttgacgt caagttggag agttttgtgg agctgacgga tgttgccaat
       490        500        510        520        530        540
gaaaagttga agtgcgcaga gacctggagc ctcaatggtc tggttaaaca cgtcttaggg
       550        560        570        580        590        600
aaacaacttt tgaaagacaa gtccatccgc tgcagcaatt ggagtaattt ccccctcact
       610        620        630        640        650        660
gaggaccaga aactgtatgc agccactgat gcttatgctg gtcttatcat ctatcaaaaa
       670        680        690        700        710        720
ttaggaaatt tgggtgatac tgcgcaagtg tttgctctaa ataaagcaga ggaaaaccta
       730        740        750        760        770        780
cctctggaga tgaagaaaca gttgaattca atctccgaag aaatgaggga cctagccaat
       790        800        810        820        830        840
cgttttcctg tcacttgcag aaatttggaa actctccaga gggttcctgt aatattgaag
       850        860        870        880        890        900
agtatttcag aaaatctctg ttcattgaga aaagtgatct gtggtcctac aaacactgag
       910        920        930        940        950        960
actagactga agccgggcag tagttttaat ttactgtcat cagaggattc agctgctgct
       970        980        990       1000       1010       1020
ggagaaaaag agaaacagat tggaaaacat agtacttttg ctaaaattaa agaagaacca
      1030       1040       1050       1060       1070       1080
tgggacccag aacttgacag tttagtgaag caagaggagg ttgatgtatt tagaaatcaa
      1090       1100       1110       1120       1130       1140
gtgaagcaag aaaaaggtga atctgaaaat gaaatagaag acaatctgtt gagagaagat
      1150       1160       1170       1180       1190       1200
atggaaagaa cttgtgtgat tcctagtatt tcagaaaatg aactccaaga tttggaacag
      1210       1220       1230       1240       1250       1260
caagctaaag aagaaaaata taatgatgtt tctcaccaac tttctgagca tttatctccc
      1270       1280       1290       1300       1310       1320
aatgatgatg agaatgactc ctcctatata attgaaagtg atgaagattt ggaaatggag
      1330       1340       1350       1360       1370       1380
atgctgaagt ctttagaaaa cctaaatagt gacgtggtgg aacccactca ctctacatgg
      1390       1400       1410       1420       1430       1440
ttggaaatgg gaaccaatgg gcgtcttcct cctgaggagg aagatggaca cggaaatgaa
      1450       1460       1470       1480       1490       1500
gccatcaaag aggagcagga agaagaggac catttattgc cggaacccaa cgcaaagcaa
      1510       1520       1530       1540       1550       1560
attaattgcc tcaagaccta tttcggacac agcagtttta aaccggttca gtggaaagtc
      1570       1580       1590       1600       1610       1620
atccattctg tattagaaga gagaagagat aatgttgttg tcatggcaac tggatatggg
      1630       1640       1650       1660       1670       1680
aagagtctgt gcttccagta tccgcctgtt tatacaggca agattggcat tgtcatttca
      1690       1700       1710       1720       1730       1740
cctctcattt ccttaatgga agaccaagtc ctccagcttg agctgtccaa tgttccagcc
      1750       1760       1770       1780       1790       1800
tgtttacttg gatctgcaca gtcaaaaaat attctaggag atgttaaatt aggcaaatat
      1810       1820       1830       1840       1850       1860
agggtcatct acataactcc agagttctgt tctggtaact tggatctact ccagcaactt
      1870       1880       1890       1900       1910       1920
gactctagta ttggcatcac tctcattgct gtggatgagg ctcactgcat ttcagagtgg
      1930       1940       1950       1960       1970       1980
ggccatgatt tcagaagttc attcaggatg ctgggctctc ttaaaacagc gctcccattg
      1990       2000       2010       2020       2030       2040
gttccagtca ttgcactctc cgctactgca agctcttcca tccgggaaga cattataagc
      2050       2060       2070       2080       2090       2100
tgcttaaacc tgaaagaccc tcagatcacc tgcactggat ttgatcggcc aaatctgtac
      2110       2120       2130       2140       2150       2160
ttagaagttg gacggaaaac agggaacatc cttcaggatc taaagccgtt tctcgtccga
      2170       2180       2190       2200       2210       2220
aaggcaagtt ctgcctggga atttgaaggt ccaaccatca tctattgtcc ttcgagaaaa
      2230       2240       2250       2260       2270       2280
atgacagaac aagttactgc tgaacttggg aaactgaact tagcctgcag aacataccac
      2290       2300       2310       2320       2330       2340
gctggcatga aaattagcga aaggaaggac gttcatcata ggttcctgag agatgaaatt
      2350       2360       2370       2380       2390       2400
cagtgtgttg tagctactgt agcttttgga atgggcatta ataaagctga cattcgcaaa
      2410       2420       2430       2440       2450       2460
gttattcatt atggtgcgcc taaggaaatg gaatcctatt accaggaaat tggtagagct
      2470       2480       2490       2500       2510       2520
ggccgggatg gacttcagag ttcctgtcac ttgctctggg ctccagcaga ctttaacaca
      2530       2540       2550       2560       2570       2580
tccaggaatc tccttattga gattcacgat gaaaagttcc ggttatataa attaaagatg
      2590       2600       2610       2620       2630       2640
atggtaaaga tggaaaaata ccttcactcc agtcagtgta ggcgacgaat catcttgtcc
      2650       2660       2670       2680       2690       2700
cattttgagg acaaatgtct gcagaaggcc tccttggaca ttatgggaac tgaaaaatgc
      2710       2720       2730       2740       2750       2760
tgtgataatt gcaggcccag gctgaatcat tgccttactg ctaacaactc agaggacgca
      2770       2780       2790       2800       2810       2820
tcccaagact ttgggccaca agcattccag ctactgtctg ctgtggacat cctgcaggag
      2830       2840       2850       2860       2870       2880
aaatttggaa ttgggattcc gatcttattt ctccgaggat ctaattctca gcgtcttcct
      2890       2900       2910       2920       2930       2940
gataaatatc ggggtcacag gctctttggt gctggaaagg agcaagcaga aagttggtgg
      2950       2960       2970       2980       2990       3000
aagacccttt ctcaccatct catagctgaa ggattcttgg tagaagttcc caaggaaaac
      3010       3020       3030       3040       3050       3060
aaatatataa agacatgttc cctcacaaaa aagggtagaa agtggcttgg agaagccagt
      3070       3080       3090       3100       3110       3120
tcgcagtctc ctccgagcct tctccttcaa gctaatgaag agatgtttcc aaggaaagtt
      3130       3140       3150       3160       3170       3180
ctgctaccaa gttctaatcc tgtatctcca gaaacgacgc aacattcctc taatcaaaac
      3190       3200       3210       3220       3230       3240
ccagctggat taactaccaa gcagtctaat ttggagagaa cgcattctta caaagtgcct
      3250       3260       3270       3280       3290       3300
gagaaagttt cttctgggac taacattcct aaaaaaagtg ccgtgatgcc gtcaccagga
      3310       3320       3330       3340       3350       3360
acatcttcca gccccttaga acctgccatc tcagcccaag agctggacgc tcggactggg
      3370       3380       3390       3400       3410       3420
ctatatgcca ggctggtgga agcaaggcag aaacacgcta ataagatgga tgtacctcca
      3430       3440       3450       3460       3470       3480
gctattttag caacaaacaa ggttctgctg gacatggcta aaatgagacc gactactgtt
      3490       3500       3510       3520       3530       3540
gaaaacatga aacagatcga cggtgtctct gaaggcaaag ctgctctgtt ggcccctctg
      3550       3560       3570       3580       3590       3600
ttggaagtca tcaaacattt ctgtcaagta actagtgttc agacagacct cctttccagt
      3610       3620       3630       3640       3650       3660
gccaaacctc acaaggaaca ggagaaaagt caggagatgg aaaagaaaga ctgctcactc
      3670       3680       3690       3700       3710       3720
ccccagtctg tggccgtcac atacactcta ttccaggaaa agaaaatgcc cttacacagc
      3730       3740       3750       3760       3770       3780
atagctgaga acaggctcct gcctctcaca gcagccggca tgcacttagc ccaggcggtg
      3790       3800       3810       3820       3830       3840
aaagccggct accccctgga tatggagcga gctggcctga ccccagagac ttggaagatt
      3850       3860       3870       3880       3890       3900
attatggatg tcatccgaaa ccctcccatc aactcagata tgtataaagt taaactcatc
      3910       3920       3930       3940       3950       3960
agaatgttag ttcctgaaaa cttagacacg tacctcatcc acatggcgat tgagattctt
      3970       3980       3990       4000       4010       4020
cagagtggtt ccgacagcag aacccagcct ccttgtgatt ccagcaggaa gaggcgtttc
      4030       4040       4050       4060       4070       4080
cccagctctg cagagagttg tgagagctgt aaggagagca aagaggcggt caccgagacc
      4090       4100       4110       4120       4130       4140
aaggcatcat cttcagagtc aaagagaaaa ttacccgagt ggtttgccaa aggaaatgtg
      4150       4160       4170       4180       4190       4200
ccctcagctg ataccggcag ctcatcatca atggccaaga ccaaaaagaa aggtctcttt

agttaa     

Last modification date: Oct. 2, 2011