Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: DNA polymerase III subunit alpha


DESCRIPTION:
DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase.

STRUCTURE SIMILARITY:
Belongs to the DNA polymerase type-C family. DnaE subfamily.


CATALYTIC ACTIVITY:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).


PROTEIN TYPE(S):
C-family DNA polymerase


RELATED PATHWAY(S):
DNA replication
mismatch repair (MMR)
homologous recombination (HR)


Amino acids sequence

        10         20         30         40         50         60
MSEPRFVHLR VHSDYSMIDG LAKTAPLVKK AAALGMPALA ITDFTNLCGL VKFYGAGHGA
        70         80         90        100        110        120
GIKPIVGADF NVQCDLLGDE LTHLTVLAAN NTGYQNLTLL ISKAYQRGYG AAGPIIDRDW
       130        140        150        160        170        180
LIELNEGLIL LSGGRMGDVG RSLLRGNSAL VDECVAFYEE HFPDRYFLEL IRTGRPDEES
       190        200        210        220        230        240
YLHAAVELAE ARGLPVVATN DVRFIDSSDF DAHEIRVAIH DGFTLDDPKR PRNYSPQQYM
       250        260        270        280        290        300
RSEEEMCELF ADIPEALANT VEIAKRCNVT VRLGEYFLPQ FPTGDMSTED YLVKRAKEGL
       310        320        330        340        350        360
EERLAFLFPD EEERLKRRPE YDERLETELQ VINQMGFPGY FLIVMEFIQW SKDNGVPVGP
       370        380        390        400        410        420
GRGSGAGSLV AYALKITDLD PLEFDLLFER FLNPERVSMP DFDVDFCMEK RDQVIEHVAD
       430        440        450        460        470        480
MYGRDAVSQI ITFGTMAAKA VIRDVGRVLG HPYGFVDRIS KLIPPDPGMT LAKAFEAEPQ
       490        500        510        520        530        540
LPEIYEADEE VKALIDMARK LEGVTRNAGK HAGGVVIAPT KITDFAPLYC DEEGKHPVTQ
       550        560        570        580        590        600
FDKSDVEYAG LVKFDFLGLR TLTIINWALE MINKRRAKNG EPPLDIAAIP LDDKKSFDML
       610        620        630        640        650        660
QRSETTAVFQ LESRGMKDLI KRLQPDCFED MIALVALFRP GPLQSGMVDN FIDRKHGREE
       670        680        690        700        710        720
ISYPDVQWQH ESLKPVLEPT YGIILYQEQV MQIAQVLSGY TLGGADMLRR AMGKKKPEEM
       730        740        750        760        770        780
AKQRSVFAEG AEKNGINAEL AMKIFDLVEK FAGYGFNKSH SAAYALVSYQ TLWLKAHYPA
       790        800        810        820        830        840
EFMAAVMTAD MDNTEKVVGL VDECWRMGLK ILPPDINSGL YHFHVNDDGE IVYGIGAIKG
       850        860        870        880        890        900
VGEGPIEAII EARNKGGYFR ELFDLCARTD TKKLNRRVLE KLIMSGAFDR LGPHRAALMN
       910        920        930        940        950        960
SLGDALKAAD QHAKAEAIGQ ADMFGVLAEE PEQIEQSYAS CQPWPEQVVL DGERETLGLY
       970        980        990       1000       1010       1020
LTGHPINQYL KEIERYVGGV RLKDMHPTER GKVITAAGLV VAARVMVTKR GNRIGICTLD
      1030       1040       1050       1060       1070       1080
DRSGRLEVML FTDALDKYQQ LLEKDRILIV SGQVSFDDFS GGLKMTAREV MDIDEAREKY
      1090       1100       1110       1120       1130       1140
ARGLAISLTD RQIDDQLLNR LRQSLEPHRS GTIPVHLYYQ RADARARLRF GATWRVSPSD
      1150       1160
RLLNDLRGLI GSEQVELEFD    

Encoded by dnaE gene


OTHER NAME(S):
ECK0183
JW0179
polC
sdgC


DESCRIPTION:
Has a PHP domin, like YcdX. [More information is available at EcoGene: EG10238]. The alpha subunit of DNA polymerase III catalyzes the polymerase activity of the holoenzyme complex . [More information is available at EcoCyc: EG10238].


Nucleic acid sequence

        10         20         30         40         50         60
atgtctgaac cacgtttcgt acacctgcgg gtgcacagcg actactcgat gatcgatggc
        70         80         90        100        110        120
ctggccaaaa ccgcaccgtt ggtaaaaaag gcggcggcgt tgggtatgcc agcactggcg
       130        140        150        160        170        180
atcaccgatt tcaccaacct ttgtggtctg gtgaagttct acggagcggg acatggcgca
       190        200        210        220        230        240
gggattaagc ctatcgtcgg ggcagatttt aacgtccagt gcgacctgct gggtgatgag
       250        260        270        280        290        300
ttaacccacc tgacggtact ggcggcgaac aataccggct atcagaatct gacgttgctg
       310        320        330        340        350        360
atctcaaaag cgtatcagcg cgggtacggt gccgccgggc cgatcatcga tcgcgactgg
       370        380        390        400        410        420
cttatcgaat taaacgaagg gttgatcctt ctttccggcg gacgcatggg cgacgtcgga
       430        440        450        460        470        480
cgcagtcttt tgcgtggtaa cagcgcgctg gtagatgagt gtgtcgcgtt ttatgaagaa
       490        500        510        520        530        540
cacttcccgg atcgctattt tctcgagctg atccgcaccg gcaggccgga tgaagaaagc
       550        560        570        580        590        600
tatctgcacg cggcggtgga actggcggaa gcgcgcggtt tgcccgtcgt ggcgaccaac
       610        620        630        640        650        660
gacgtgcgct ttatcgacag cagcgacttt gacgcacacg aaatccgcgt cgcgatccac
       670        680        690        700        710        720
gacggcttta ccctcgacga tcctaaacgc ccgcgtaact attcgccgca gcaatatatg
       730        740        750        760        770        780
cgtagcgaag aggagatgtg tgagctgttt gccgacatcc ccgaagccct tgccaacacc
       790        800        810        820        830        840
gttgagatcg ccaaacgctg taacgtaacc gtgcgtcttg gtgaatactt cctgccgcag
       850        860        870        880        890        900
ttcccgaccg gggacatgag caccgaagat tatctggtca agcgtgcaaa agagggcctg
       910        920        930        940        950        960
gaagagcgtc tggccttttt attccctgat gaggaagaac gtcttaagcg ccgcccggaa
       970        980        990       1000       1010       1020
tatgacgaac gtctggagac tgaacttcag gttatcaacc agatgggctt cccgggctac
      1030       1040       1050       1060       1070       1080
ttcctcatcg ttatggaatt tatccagtgg tcgaaagata acggcgtacc ggtagggcca
      1090       1100       1110       1120       1130       1140
ggccgtggct ccggtgcggg ttcactggtg gcctacgcgc tgaaaatcac cgacctcgat
      1150       1160       1170       1180       1190       1200
ccgctggaat ttgacctgct gttcgaacgt ttccttaacc cggaacgtgt ctccatgcct
      1210       1220       1230       1240       1250       1260
gacttcgacg ttgacttctg tatggagaaa cgcgatcagg ttatcgagca cgtagcggac
      1270       1280       1290       1300       1310       1320
atgtacggtc gtgatgcggt atcgcagatc atcaccttcg gtacaatggc ggcgaaagcg
      1330       1340       1350       1360       1370       1380
gtgatccgcg acgtaggccg cgtgctgggg catccgtacg gctttgtcga tcgtatctcg
      1390       1400       1410       1420       1430       1440
aaactgatcc cgcccgatcc ggggatgacg ctggcgaaag cgtttgaagc cgagccgcag
      1450       1460       1470       1480       1490       1500
ctgccggaaa tctacgaagc ggatgaagaa gttaaggcgc tgatcgacat ggcgcgcaaa
      1510       1520       1530       1540       1550       1560
ctggaagggg tcacccgtaa cgccggtaag cacgccggtg gggtggttat cgcgccgacc
      1570       1580       1590       1600       1610       1620
aaaattaccg attttgcgcc gctttactgc gatgaagagg gcaaacatcc ggtcacccag
      1630       1640       1650       1660       1670       1680
tttgataaaa gcgacgttga atacgccgga ctggtgaagt tcgacttcct tggtttgcgt
      1690       1700       1710       1720       1730       1740
acgctcacca tcatcaactg ggcgctggag atgatcaaca agcggcgggc gaagaatggc
      1750       1760       1770       1780       1790       1800
gagccgccgc tggatatcgc tgcgatcccg ctggatgata agaaaagctt cgacatgctg
      1810       1820       1830       1840       1850       1860
caacgctcgg aaaccacggc ggtattccag cttgaatcgc gcggcatgaa ggacctgatc
      1870       1880       1890       1900       1910       1920
aagcgtctac aacctgactg cttcgaagat atgatcgccc tagtggcact gttccgcccc
      1930       1940       1950       1960       1970       1980
ggtccgttgc aatcagggat ggtggataac tttatcgacc gtaaacatgg tcgtgaagag
      1990       2000       2010       2020       2030       2040
atctcctatc cggacgtaca gtggcagcat gaaagcctga aaccggtact ggagccaacc
      2050       2060       2070       2080       2090       2100
tacggcatta tcctgtatca ggaacaggtc atgcagattg cgcaggtgct ttctggttat
      2110       2120       2130       2140       2150       2160
accctcggtg gcgcggatat gctgcgtcgt gcgatgggta agaaaaagcc ggaagagatg
      2170       2180       2190       2200       2210       2220
gctaagcaac gttctgtatt tgctgaaggt gcagaaaaga acggaatcaa cgctgaactg
      2230       2240       2250       2260       2270       2280
gcgatgaaaa tcttcgacct ggtggagaaa ttcgctggtt acggatttaa caaatcgcac
      2290       2300       2310       2320       2330       2340
tctgcggcct atgctttggt gtcatatcaa acgttatggc tgaaagcgca ctatcctgcg
      2350       2360       2370       2380       2390       2400
gagtttatgg cggcggtaat gaccgccgat atggacaaca ccgagaaggt ggtgggtctg
      2410       2420       2430       2440       2450       2460
gtggatgagt gctggcggat ggggctgaaa atcctgccac cagatataaa ctccggtctt
      2470       2480       2490       2500       2510       2520
taccatttcc acgtcaacga cgacggcgaa atcgtgtatg gtattggcgc gatcaaaggg
      2530       2540       2550       2560       2570       2580
gtcggtgaag gtccgattga ggccatcatc gaagcccgta ataaaggcgg ctacttccgc
      2590       2600       2610       2620       2630       2640
gaactgtttg atctctgcgc ccgtaccgac accaaaaagt tgaaccgtcg cgtgctggaa
      2650       2660       2670       2680       2690       2700
aaactgatca tgtccggggc gtttgaccgt cttgggccac atcgcgcagc gctgatgaac
      2710       2720       2730       2740       2750       2760
tcgctgggcg atgcgttaaa agcggcagat caacacgcga aagcggaagc tatcggtcag
      2770       2780       2790       2800       2810       2820
gccgatatgt tcggcgtgct ggccgaagag ccggaacaaa ttgaacaatc ctacgccagc
      2830       2840       2850       2860       2870       2880
tgccaaccgt ggccggagca ggtggtatta gatggggaac gtgaaacgtt aggcctgtac
      2890       2900       2910       2920       2930       2940
ctgaccggac accctatcaa ccagtattta aaagagattg agcgttatgt cggaggcgta
      2950       2960       2970       2980       2990       3000
aggctgaaag acatgcaccc gacagaacgt ggtaaagtca tcacggctgc ggggctcgtt
      3010       3020       3030       3040       3050       3060
gttgccgcgc gggttatggt caccaagcgc ggcaatcgta tcggtatctg cacgctggat
      3070       3080       3090       3100       3110       3120
gaccgttccg ggcggctgga agtgatgttg tttactgacg ccctggataa ataccagcaa
      3130       3140       3150       3160       3170       3180
ttgctggaaa aagaccgcat acttatcgtc agcggacagg tcagctttga tgacttcagc
      3190       3200       3210       3220       3230       3240
ggtgggctta aaatgaccgc tcgcgaagtg atggatattg acgaagcccg ggaaaaatat
      3250       3260       3270       3280       3290       3300
gctcgcgggc ttgctatctc gctgacggac aggcaaattg atgaccagct tttaaaccga
      3310       3320       3330       3340       3350       3360
ctccgtcagt ctctggaacc ccaccgctct gggacaattc cagtacatct ctactatcag
      3370       3380       3390       3400       3410       3420
agggcggatg cacgcgcgcg gttgcgtttt ggcgcgacgt ggcgtgtctc tccgagcgat
      3430       3440       3450       3460       3470       3480
cgtttattaa acgatctccg tggcctcatt ggttcggagc aggtggaact ggagtttgac

taa     

Last modification date: Oct. 2, 2011