Homo sapiens L. (human) [HSA]

FULL NAME: DNA polymerase alpha catalytic subunit


DESCRIPTION:
Plays an essential role in the initiation of DNA replication. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1/p180, a regulatory subunit POLA2/p70 and two primase subunits PRIM1/p49 and PRIM2/p58) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes.

MISCELLANEOUS:
In eukaryotes there are five DNA polymerases: alpha, beta, gamma, delta, and epsilon which are responsible for different reactions of DNA synthesis.


STRUCTURE SIMILARITY:
Belongs to the DNA polymerase type-B family.


CATALYTIC ACTIVITY:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).


POST-TRANSLATIONAL MODIFICATION:
A 165 kDa form is probably produced by proteolytic cleavage at Lys-124.


PROTEIN TYPE(S):
B-family DNA polymerase


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MAPVHGDDSL SDSGSFVSSR ARREKKSKKG RQEALERLKK AKAGEKYKYE VEDFTGVYEE
        70         80         90        100        110        120
VDEEQYSKLV QARQDDDWIV DDDGIGYVED GREIFDDDLE DDALDADEKG KDGKARNKDK
       130        140        150        160        170        180
RNVKKLAVTK PNNIKSMFIA CAGKKTADKA VDLSKDGLLG DILQDLNTET PQITPPPVMI
       190        200        210        220        230        240
LKKKRSIGAS PNPFSVHTAT AVPSGKIASP VSRKEPPLTP VPLKRAEFAG DDVQVESTEE
       250        260        270        280        290        300
EQESGAMEFE DGDFDEPMEV EEVDLEPMAA KAWDKESEPA EEVKQEADSG KGTVSYLGSF
       310        320        330        340        350        360
LPDVSCWDID QEGDSSFSVQ EVQVDSSHLP LVKGADEEQV FHFYWLDAYE DQYNQPGVVF
       370        380        390        400        410        420
LFGKVWIESA ETHVSCCVMV KNIERTLYFL PREMKIDLNT GKETGTPISM KDVYEEFDEK
       430        440        450        460        470        480
IATKYKIMKF KSKPVEKNYA FEIPDVPEKS EYLEVKYSAE MPQLPQDLKG ETFSHVFGTN
       490        500        510        520        530        540
TSSLELFLMN RKIKGPCWLE VKSPQLLNQP VSWCKVEAMA LKPDLVNVIK DVSPPPLVVM
       550        560        570        580        590        600
AFSMKTMQNA KNHQNEIIAM AALVHHSFAL DKAAPKPPFQ SHFCVVSKPK DCIFPYAFKE
       610        620        630        640        650        660
VIEKKNVKVE VAATERTLLG FFLAKVHKID PDIIVGHNIY GFELEVLLQR INVCKAPHWS
       670        680        690        700        710        720
KIGRLKRSNM PKLGGRSGFG ERNATCGRMI CDVEISAKEL IRCKSYHLSE LVQQILKTER
       730        740        750        760        770        780
VVIPMENIQN MYSESSQLLY LLEHTWKDAK FILQIMCELN VLPLALQITN IAGNIMSRTL
       790        800        810        820        830        840
MGGRSERNEF LLLHAFYENN YIVPDKQIFR KPQQKLGDED EEIDGDTNKY KKGRKKAAYA
       850        860        870        880        890        900
GGLVLDPKVG FYDKFILLLD FNSLYPSIIQ EFNICFTTVQ RVASEAQKVT EDGEQEQIPE
       910        920        930        940        950        960
LPDPSLEMGI LPREIRKLVE RRKQVKQLMK QQDLNPDLIL QYDIRQKALK LTANSMYGCL
       970        980        990       1000       1010       1020
GFSYSRFYAK PLAALVTYKG REILMHTKEM VQKMNLEVIY GDTDSIMINT NSTNLEEVFK
      1030       1040       1050       1060       1070       1080
LGNKVKSEVN KLYKLLEIDI DGVFKSLLLL KKKKYAALVV EPTSDGNYVT KQELKGLDIV
      1090       1100       1110       1120       1130       1140
RRDWCDLAKD TGNFVIGQIL SDQSRDTIVE NIQKRLIEIG ENVLNGSVPV SQFEINKALT
      1150       1160       1170       1180       1190       1200
KDPQDYPDKK SLPHVHVALW INSQGGRKVK AGDTVSYVIC QDGSNLTASQ RAYAPEQLQK
      1210       1220       1230       1240       1250       1260
QDNLTIDTQY YLAQQIHPVV ARICEPIDGI DAVLIATWLG LDPTQFRVHH YHKDEENDAL
      1270       1280       1290       1300       1310       1320
LGGPAQLTDE EKYRDCERFK CPCPTCGTEN IYDNVFDGSG TDMEPSLYRC SNIDCKASPL
      1330       1340       1350       1360       1370       1380
TFTVQLSNKL IMDIRRFIKK YYDGWLICEE PTCRNRTRHL PLQFSRTGPL CPACMKATLQ
      1390       1400       1410       1420       1430       1440
PEYSDKSLYT QLCFYRYIFD AECALEKLTT DHEKDKLKKQ FFTPKVLQDY RKLKNTAEQF
      1450       1460
LSRSGYSEVN LSKLFAGCAV KS   

Encoded by POLA1 gene

FULL NAME: polymerase (DNA directed), alpha 1, catalytic subunit


OTHER NAME(S):
DKFZp686K1672
POLA
p180


DESCRIPTION:
This gene encodes the catalytic subunit of DNA polymerase, which together with a regulatory and two primase subunits, forms the DNA polymerase alpha complex. The catalytic subunit plays an essential role in the initiation of DNA replication. [provided by RefSeq, Mar 2010]


Nucleic acid sequence

        10         20         30         40         50         60
atggcacctg tgcacggcga cgactctctg tcagattcag ggagttttgt atcttctcga
        70         80         90        100        110        120
gcccggcgag aaaaaaaatc aaagaagggg cgccaagaag ccctagaaag actgaaaaag
       130        140        150        160        170        180
gctaaagctg gtgagaagta taaatatgaa gtcgaggact tcacaggtgt ttatgaagaa
       190        200        210        220        230        240
gttgatgaag aacagtattc gaagctggtt caggcacgcc aggatgatga ctggattgtg
       250        260        270        280        290        300
gatgatgatg gtattggcta tgtggaagat ggccgagaga tttttgatga tgaccttgaa
       310        320        330        340        350        360
gatgatgccc ttgatgctga tgagaaagga aaagatggta aagcacgcaa taaagacaag
       370        380        390        400        410        420
aggaatgtaa agaagctcgc agtgacaaaa ccgaacaaca ttaagtcaat gttcattgct
       430        440        450        460        470        480
tgtgctggaa agaaaactgc agataaagct gtagacttgt ccaaggatgg tctgctaggt
       490        500        510        520        530        540
gacattctac aggatcttaa cactgagaca cctcaaataa ctccaccacc tgtaatgata
       550        560        570        580        590        600
ctgaagaaga aaagatccat tggagcttca ccgaatcctt tctctgtgca caccgccacg
       610        620        630        640        650        660
gcagttcctt caggaaaaat tgcttcccct gtctccagaa aggagcctcc attaactcct
       670        680        690        700        710        720
gttcctctta aacgtgctga atttgctggc gatgatgtac aggtcgagag tacagaagaa
       730        740        750        760        770        780
gagcaggagt caggggcaat ggagtttgaa gatggtgact ttgatgagcc catggaagtt
       790        800        810        820        830        840
gaagaggtgg acctggagcc tatggctgcc aaggcttggg acaaagagag tgagccagca
       850        860        870        880        890        900
gaggaagtga aacaagaggc ggattctggg aaagggaccg tgtcctactt aggaagtttt
       910        920        930        940        950        960
ctcccggatg tctcttgttg ggacattgat caagaaggtg atagcagttt ctcagtgcaa
       970        980        990       1000       1010       1020
gaagttcaag tggattccag tcacctccca ttggtaaaag gggcagatga ggaacaagta
      1030       1040       1050       1060       1070       1080
ttccactttt attggttgga tgcttatgag gatcagtaca accaaccagg tgtggtattt
      1090       1100       1110       1120       1130       1140
ctgtttggga aagtttggat tgaatcagcc gagacccatg tgagctgttg tgtcatggtg
      1150       1160       1170       1180       1190       1200
aaaaatatcg agcgaacgct ttacttcctt ccccgtgaaa tgaaaattga tctaaatacg
      1210       1220       1230       1240       1250       1260
gggaaagaaa caggaactcc aatttcaatg aaggatgttt atgaggaatt tgatgagaaa
      1270       1280       1290       1300       1310       1320
atagcaacaa aatataaaat tatgaagttc aagtctaagc cagtggaaaa gaactatgct
      1330       1340       1350       1360       1370       1380
tttgagatac ctgatgttcc agaaaaatct gagtacttgg aagttaaata ctcggctgaa
      1390       1400       1410       1420       1430       1440
atgccacagc ttcctcaaga tttgaaagga gaaacttttt ctcatgtatt tgggaccaac
      1450       1460       1470       1480       1490       1500
acatctagcc tggaactgtt cttgatgaac agaaagatca aaggaccttg ttggcttgaa
      1510       1520       1530       1540       1550       1560
gtaaaaagtc cacagctctt gaatcagcca gtcagttggt gtaaagttga ggcaatggct
      1570       1580       1590       1600       1610       1620
ttgaaaccag acctggtgaa tgtaattaag gatgtcagtc caccaccgct tgtcgtgatg
      1630       1640       1650       1660       1670       1680
gctttcagca tgaagacaat gcagaatgca aagaaccatc aaaatgagat tattgctatg
      1690       1700       1710       1720       1730       1740
gcagctttgg tccatcacag ttttgcattg gataaagcag ccccaaagcc tccctttcag
      1750       1760       1770       1780       1790       1800
tcacacttct gtgttgtgtc taaaccaaag gactgtattt ttccatatgc tttcaaagaa
      1810       1820       1830       1840       1850       1860
gtcattgaga aaaagaatgt gaaggttgag gttgctgcaa cagaaagaac actgctaggt
      1870       1880       1890       1900       1910       1920
tttttccttg caaaagttca caaaattgat cctgatatca ttgtgggtca taatatttat
      1930       1940       1950       1960       1970       1980
gggtttgaac tggaagtact actgcagaga attaatgtgt gcaaagctcc tcactggtcc
      1990       2000       2010       2020       2030       2040
aagataggtc gactgaagcg atccaacatg ccaaagcttg ggggccggag tggatttggt
      2050       2060       2070       2080       2090       2100
gaaagaaatg ctacctgtgg tcgaatgatc tgtgatgtgg aaatttcagc aaaggaattg
      2110       2120       2130       2140       2150       2160
attcgttgta aaagctacca tctgtctgaa cttgttcagc agattctaaa aactgaaagg
      2170       2180       2190       2200       2210       2220
gttgtaatcc caatggaaaa tatacaaaat atgtacagtg aatcttctca actgttatac
      2230       2240       2250       2260       2270       2280
ctgttggaac acacctggaa agatgccaag ttcattttgc agatcatgtg tgagctaaat
      2290       2300       2310       2320       2330       2340
gttcttccat tagcattgca gatcactaac atcgctggga acattatgtc caggacgctg
      2350       2360       2370       2380       2390       2400
atgggtggac gatccgagcg taacgagttc ttgttgcttc atgcatttta cgaaaacaac
      2410       2420       2430       2440       2450       2460
tatattgtgc ctgacaagca gattttcaga aagcctcagc aaaaactggg agatgaagat
      2470       2480       2490       2500       2510       2520
gaagaaattg atggagatac caataaatac aagaaaggac gtaagaaagc agcttatgct
      2530       2540       2550       2560       2570       2580
ggaggcttgg ttttggaccc caaagttggt ttttatgata agttcatttt gcttctggac
      2590       2600       2610       2620       2630       2640
ttcaacagtc tatatccttc catcattcag gaatttaaca tttgttttac aacagtacaa
      2650       2660       2670       2680       2690       2700
agagttgctt cagaggcaca gaaagttaca gaggatggag aacaagaaca gatccctgag
      2710       2720       2730       2740       2750       2760
ttgccagatc caagcttaga aatgggcatt ttgcccagag agatccggaa actggtagaa
      2770       2780       2790       2800       2810       2820
cggagaaaac aagtcaaaca gctaatgaaa cagcaagact taaatccaga ccttattctt
      2830       2840       2850       2860       2870       2880
cagtatgaca ttcgacagaa ggctttgaag ctcacagcga acagtatgta tggttgcctg
      2890       2900       2910       2920       2930       2940
ggattttcct atagcagatt ttacgccaaa ccactggctg ccttggtgac atacaaagga
      2950       2960       2970       2980       2990       3000
agggagattt tgatgcatac gaaagagatg gtacaaaaga tgaatcttga agttatttat
      3010       3020       3030       3040       3050       3060
ggagatacag attcaattat gataaacacc aatagcacca atctggaaga agtatttaag
      3070       3080       3090       3100       3110       3120
ttgggaaaca aggtaaaaag tgaagtgaat aagttgtaca aactgcttga aatagacatt
      3130       3140       3150       3160       3170       3180
gatggggttt tcaagtctct gctactgctg aaaaaaaaga agtacgctgc tctggttgtt
      3190       3200       3210       3220       3230       3240
gagccaacgt cggatgggaa ttatgtcacc aaacaggagc tcaaaggatt agatatagtt
      3250       3260       3270       3280       3290       3300
agaagagatt ggtgtgatct tgctaaagac actggaaact ttgtgattgg ccagattctt
      3310       3320       3330       3340       3350       3360
tctgatcaaa gccgggacac tatagtggaa aacattcaga agaggctgat agaaattgga
      3370       3380       3390       3400       3410       3420
gaaaatgtgc taaatggcag tgtcccagtg agccagtttg aaattaacaa ggcattgaca
      3430       3440       3450       3460       3470       3480
aaggatcccc aggattaccc tgataaaaaa agcctacctc atgtacatgt tgccctctgg
      3490       3500       3510       3520       3530       3540
ataaattctc aaggaggcag aaaggtgaaa gctggagata ctgtgtcata tgtcatctgt
      3550       3560       3570       3580       3590       3600
caggatggat caaacctcac tgcaagtcag agggcctatg cgcctgagca gctgcagaaa
      3610       3620       3630       3640       3650       3660
caggataatc taaccattga cacccagtac tacctggccc agcagatcca cccagtcgtg
      3670       3680       3690       3700       3710       3720
gctcggatct gtgaaccaat agacggaatt gatgctgtcc tcattgcaac gtggttggga
      3730       3740       3750       3760       3770       3780
cttgacccca cccaatttag agttcatcat tatcataaag atgaagagaa tgatgctcta
      3790       3800       3810       3820       3830       3840
cttggtggcc cagcacagct cactgatgaa gagaaataca gggactgtga aagattcaaa
      3850       3860       3870       3880       3890       3900
tgtccatgcc ctacatgtgg aactgagaat atttatgata atgtctttga tggttcggga
      3910       3920       3930       3940       3950       3960
acagatatgg agcccagctt gtatcgttgc agtaacatcg attgtaaggc ttcacctctg
      3970       3980       3990       4000       4010       4020
acctttacag tacaactgag caacaaattg atcatggaca ttagacgttt cattaaaaag
      4030       4040       4050       4060       4070       4080
tactatgatg gctggttgat atgtgaagag ccaacctgtc gcaatcgaac tcgtcacctt
      4090       4100       4110       4120       4130       4140
ccccttcaat tctcccgaac tgggcctctt tgcccagcct gcatgaaagc tacacttcaa
      4150       4160       4170       4180       4190       4200
ccagagtatt ctgacaagtc cctgtacacc cagctgtgct tttaccggta catttttgat
      4210       4220       4230       4240       4250       4260
gcggagtgtg cactggagaa acttactacc gatcatgaga aagataaatt gaagaagcaa
      4270       4280       4290       4300       4310       4320
ttttttaccc ccaaagttct gcaggactac agaaaactca agaacacagc agagcaattc
      4330       4340       4350       4360       4370       4380
ttgtcccgaa gtggctactc cgaagtgaat ctgagcaaac tcttcgctgg ttgtgccgtg

aaatcctaa     

Last modification date: Oct. 2, 2011