Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: DNA repair helicase RAD25


DESCRIPTION:
Probably an ATP-dependent DNA helicase, which may have a DNA unwinding function. Has an essential function in translation initiation. Acts as component of the general transcription and DNA repair factor IIH (TFIIH) core, which is essential for both basal and activated transcription, and is involved in nucleotide excision repair (NER) of damaged DNA. TFIIH has CTD kinase and DNA-dependent ATPase activity, and is essential for polymerase II transcription in vitro.

MISCELLANEOUS:
A C-terminal deletion renders yeast hypersensitive to UV light.
Present with 825 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Contains 1 helicase C-terminal domain.
Contains 1 helicase ATP-binding domain.
Belongs to the helicase family. RAD25/XPB subfamily.


CATALYTIC ACTIVITY:
ATP + H(2)O = ADP + phosphate.


RELATED PATHWAY(S):
nucleotide excision repair (NER)
transcription factors


Amino acids sequence

        10         20         30         40         50         60
MTDVEGYQPK SKGKIFPDMG ESFFSSDEDS PATDAEIDEN YDDNRETSEG RGERDTGAMV
        70         80         90        100        110        120
TGLKKPRKKT KSSRHTAADS SMNQMDAKDK ALLQDTNSDI PADFVPDSVS GMFRSHDFSY
       130        140        150        160        170        180
LRLRPDHASR PLWISPSDGR IILESFSPLA EQAQDFLVTI AEPISRPSHI HEYKITAYSL
       190        200        210        220        230        240
YAAVSVGLET DDIISVLDRL SKVPVAESII NFIKGATISY GKVKLVIKHN RYFVETTQAD
       250        260        270        280        290        300
ILQMLLNDSV IGPLRIDSDH QVQPPEDVLQ QQLQQTAGKP ATNVNPNDVE AVFSAVIGGD
       310        320        330        340        350        360
NEREEEDDDI DAVHSFEIAN ESVEVVKKRC QEIDYPVLEE YDFRNDHRNP DLDIDLKPST
       370        380        390        400        410        420
QIRPYQEKSL SKMFGNGRAR SGIIVLPCGA GKTLVGITAA CTIKKSVIVL CTSSVSVMQW
       430        440        450        460        470        480
RQQFLQWCTL QPENCAVFTS DNKEMFQTES GLVVSTYSMV ANTRNRSHDS QKVMDFLTGR
       490        500        510        520        530        540
EWGFIILDEV HVVPAAMFRR VVSTIAAHAK LGLTATLVRE DDKIGDLNFL IGPKLYEANW
       550        560        570        580        590        600
MELSQKGHIA NVQCAEVWCP MTAEFYQEYL RETARKRMLL YIMNPTKFQA CQFLIQYHER
       610        620        630        640        650        660
RGDKIIVFSD NVYALQEYAL KMGKPFIYGS TPQQERMNIL QNFQYNDQIN TIFLSKVGDT
       670        680        690        700        710        720
SIDLPEATCL IQISSHYGSR RQEAQRLGRI LRAKRRNDEG FNAFFYSLVS KDTQEMYYST
       730        740        750        760        770        780
KRQAFLVDQG YAFKVITHLH GMENIPNLAY ASPRERRELL QEVLLKNEEA AGIEVGDDAD
       790        800        810        820        830        840
NSVGRGSNGH KRFKSKAVRG EGSLSGLAGG EDMAYMEYST NKNKELKEHH PLIRKMYYKN

LKK     

Encoded by SSL2 gene


OTHER NAME(S):
LOM3
RAD25


Nucleic acid sequence

        10         20         30         40         50         60
atgacggacg ttgaaggcta ccaacctaag agcaagggga aaatctttcc agatatgggt
        70         80         90        100        110        120
gagtcctttt tctcatcaga tgaggattca ccagctactg atgcagagat cgatgagaac
       130        140        150        160        170        180
tatgatgata acagggaaac gtcagaagga cgtggagaaa gggataccgg cgccatggta
       190        200        210        220        230        240
acagggttga agaagcctcg gaaaaagacc aagagtagca ggcatacagc ggcggattcc
       250        260        270        280        290        300
tctatgaatc aaatggacgc taaggataaa gcgcttttgc aggacactaa tagcgatata
       310        320        330        340        350        360
ccagcagatt tcgttccaga ttccgtctct ggaatgttca gaagccatga ctttagttat
       370        380        390        400        410        420
ttgcggttaa gaccagacca tgcttccagg cccctttgga tctcgccaag tgacggtagg
       430        440        450        460        470        480
atcattctgg agagtttctc tccattagca gaacaggctc aggactttct ggtcaccatt
       490        500        510        520        530        540
gcagagccta taagtagacc ctcccatatt catgaataca agatcacagc atactctttg
       550        560        570        580        590        600
tatgcagctg tttctgtagg gttggagacg gatgatatca tttcagttct ggatagatta
       610        620        630        640        650        660
tccaaagtac ccgttgctga atcgataatc aacttcatta aaggggctac catttcatac
       670        680        690        700        710        720
ggtaaagtga aactagttat taagcataat agatatttcg tggagactac acaagcagat
       730        740        750        760        770        780
attctgcaaa tgttgctgaa tgattccgtc atcgggcccc taagaataga tagcgatcat
       790        800        810        820        830        840
caagtacagc caccagagga tgtactacag caacaacttc agcaaaccgc tggaaaaccc
       850        860        870        880        890        900
gctactaatg ttaatccgaa cgatgtggaa gccgtgttta gtgcagttat aggtggtgat
       910        920        930        940        950        960
aatgagcggg aagaagaaga tgatgatatt gatgccgttc actcctttga aattgccaat
       970        980        990       1000       1010       1020
gagtctgttg aagtcgtaaa gaaaagatgt caagaaatcg attatcccgt gttggaagaa
      1030       1040       1050       1060       1070       1080
tacgacttta gaaacgacca tagaaacccg gatttagata ttgatttgaa accttccact
      1090       1100       1110       1120       1130       1140
caaatcagac cctatcaaga aaaatcgctg agtaaaatgt ttggtaatgg gcgtgctcgt
      1150       1160       1170       1180       1190       1200
tcagggatta ttgttttgcc atgtggcgca ggtaaaactt tggtcggtat taccgcagca
      1210       1220       1230       1240       1250       1260
tgtactataa aaaaatccgt aattgtttta tgtacttcat cagtctccgt catgcaatgg
      1270       1280       1290       1300       1310       1320
aggcagcaat ttttacaatg gtgtacctta caacctgaaa attgtgctgt tttcacctct
      1330       1340       1350       1360       1370       1380
gataataagg aaatgttcca gacagaatcc ggtttggttg tttccactta ttcaatggtg
      1390       1400       1410       1420       1430       1440
gcaaacacaa gaaacaggtc tcatgattca caaaaagtta tggatttctt gaccggtagg
      1450       1460       1470       1480       1490       1500
gaatgggggt tcattattct tgacgaagtt catgtggtgc ccgccgcaat gttccgtagg
      1510       1520       1530       1540       1550       1560
gtggtctcca ccatcgcagc acacgccaaa ttgggactga ccgcaacgct ggttagagaa
      1570       1580       1590       1600       1610       1620
gatgataaaa ttggtgatct gaatttttta attggtccaa aactttatga agcaaattgg
      1630       1640       1650       1660       1670       1680
atggaattat cccaaaaagg acatattgca aatgtccaat gtgcagaggt ttggtgtccc
      1690       1700       1710       1720       1730       1740
atgacagcag aattttacca agagtattta agagaaactg caaggaaaag aatgctactg
      1750       1760       1770       1780       1790       1800
tatatcatga atccaacaaa gtttcaggca tgccaattct taattcaata tcacgaaagg
      1810       1820       1830       1840       1850       1860
aggggtgata agatcatcgt tttttcagat aatgtttacg ccttacaaga atatgctttg
      1870       1880       1890       1900       1910       1920
aaaatgggaa aaccatttat ttatggttcc acaccacaac aagagcgtat gaacattctg
      1930       1940       1950       1960       1970       1980
caaaatttcc agtacaatga ccaaatcaat accatatttc tatcaaaagt tggtgatact
      1990       2000       2010       2020       2030       2040
tccatcgatt taccagaagc tacctgttta attcaaatat cttcgcacta tgggtctcgt
      2050       2060       2070       2080       2090       2100
cgtcaagaag cccaaagatt aggaagaatt ttaagagcta aaagacgtaa tgacgaaggt
      2110       2120       2130       2140       2150       2160
ttcaatgcgt tcttttattc tctggtttct aaggatacgc aagaaatgta ctactcaaca
      2170       2180       2190       2200       2210       2220
aagagacaag cgtttttggt agatcaaggt tacgcattca aagtcattac acatttacac
      2230       2240       2250       2260       2270       2280
ggaatggaga acattccaaa tttggcatat gcttcaccca gagaacgtag agagctattg
      2290       2300       2310       2320       2330       2340
caagaagtat tattgaagaa cgaagaagct gctggtatcg aggtcggtga tgacgctgac
      2350       2360       2370       2380       2390       2400
aattctgttg ggagaggttc aaatggccac aaaagattta aatcaaaggc cgttagaggt
      2410       2420       2430       2440       2450       2460
gaaggttcat tgtctggtct tgctggcggt gaagatatgg catatatgga atacagtacc
      2470       2480       2490       2500       2510       2520
aataaaaaca aagaactgaa ggaacatcat ccattaatca gaaagatgta ttataagaat
      2530
ttgaagaagt ga    

Last modification date: Oct. 2, 2011