Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: Serine/threonine-protein kinase KIN28


DESCRIPTION:
Catalytic component of the TFIIK complex (KIN28-CCL1 dimer) which is the protein kinase component of transcription factor IIH (TFIIH) and phosphorylates the C-terminal domain of RNA polymerase II during transition from transcription to elongation after preinitiation complex (PIC) formation, thereby positively regulating transcription. TFIIH (or factor B) is essential for both basal and activated transcription, and is involved in nucleotide excision repair (NER) of damaged DNA. TFIIH has DNA-dependent ATPase activity and is essential for polymerase II transcription in vitro. Essential for cell proliferation.

MISCELLANEOUS:
Present with 4400 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.
Contains 1 protein kinase domain.


CATALYTIC ACTIVITY:
ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate.


POST-TRANSLATIONAL MODIFICATION:
Phosphorylation of Thr-162 regulates the affinity of interaction between CCL1, KIN28 and TFB3. Thr-162 phosphorylation does not vary through the cell cycle and is necessary for full kinase activity.


RELATED PATHWAY(S):
nucleotide excision repair (NER)
transcription factors


Amino acids sequence

        10         20         30         40         50         60
MKVNMEYTKE KKVGEGTYAV VYLGCQHSTG RKIAIKEIKT SEFKDGLDMS AIREVKYLQE
        70         80         90        100        110        120
MQHPNVIELI DIFMAYDNLN LVLEFLPTDL EVVIKDKSIL FTPADIKAWM LMTLRGVYHC
       130        140        150        160        170        180
HRNFILHRDL KPNNLLFSPD GQIKVADFGL ARAIPAPHEI LTSNVVTRWY RAPELLFGAK
       190        200        210        220        230        240
HYTSAIDIWS VGVIFAELML RIPYLPGQND VDQMEVTFRA LGTPTDRDWP EVSSFMTYNK
       250        260        270        280        290        300
LQIYPPPSRD ELRKRFIAAS EYALDFMCGM LTMNPQKRWT AVQCLESDYF KELPPPSDPS

SIKIRN     

Encoded by KIN28 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgaaagtga atatggagta cacaaaggaa aagaaagttg gtgagggtac ttatgcggtt
        70         80         90        100        110        120
gtttacttgg gttgtcaaca ctctactgga agaaagattg ctattaagga gatcaaaaca
       130        140        150        160        170        180
tccgaattta aagatggttt agatatgtca gctatccgtg aagttaagta cctccaagaa
       190        200        210        220        230        240
atgcagcatc cgaacgtcat agaactaata gacatattta tggcttatga taatttaaat
       250        260        270        280        290        300
ctcgttctgg agttcctacc aactgatcta gaggtggtaa taaaagacaa atcaatactg
       310        320        330        340        350        360
tttacaccag cagatattaa ggcatggatg cttatgactt tgaggggcgt gtatcattgc
       370        380        390        400        410        420
cacagaaatt tcattttgca cagggatctg aaaccaaaca atttattatt ttcacctgat
       430        440        450        460        470        480
ggccagataa aagtagcaga tttcggtcta gcaagggcga taccggcccc acatgagata
       490        500        510        520        530        540
ctgacaagta acgtcgtaac aagatggtat agagcgccag aattgttgtt tggagctaaa
       550        560        570        580        590        600
cattacacat cggctattga tatctggtca gtaggcgtta tattcgcgga attaatgcta
       610        620        630        640        650        660
aggatacctt atttaccagg acagaatgat gtcgatcaaa tggaagtaac gttcagggcc
       670        680        690        700        710        720
ttagggacac ctacagatag agattggccc gaagtttctt cctttatgac gtataacaag
       730        740        750        760        770        780
ttacaaatat atccgccccc ttcaagagat gaattgagga aaaggttcat tgctgctagc
       790        800        810        820        830        840
gaatacgcct tagattttat gtgtggaatg ctaacgatga acccacaaaa gaggtggacc
       850        860        870        880        890        900
gctgttcagt gtttagaaag tgattatttc aaagaattac caccaccaag tgacccgtct
       910        920
tcaataaaaa tacgtaactg a   

Last modification date: Oct. 2, 2011