Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: UvrABC system protein B


DESCRIPTION:
The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon binding of the uvrA(2)B(2) complex to a putative damaged site, the DNA wraps around one uvrB monomer. DNA wrap is dependent on ATP binding by uvrB and probably causes local melting of the DNA helix, facilitating insertion of uvrB beta-hairpin between the DNA strands. Then uvrB probes one DNA strand for the presence of a lesion. If a lesion is found the uvrA subunits dissociate and the uvrB-DNA preincision complex is formed. This complex is subsequently bound by uvrC and the second uvrB is released. If no lesion is found, the DNA wraps around the other uvrB subunit that will check the other stand for damage.

MISCELLANEOUS:
According to PubMed:3515321, a cleaved form of the protein was observed that resulted from the removal of about 40 amino acids from the C-terminus of the protein. The exact cleavage site being unknown, it was proposed to be between Lys-630 and Ala-631. There was no indication that cleavage occured in vivo and therefore it is not known if it has any physiological significance.


STRUCTURE SIMILARITY:
Belongs to the uvrB family.
Contains 1 helicase C-terminal domain.
Contains 1 helicase ATP-binding domain.
Contains 1 UVR domain.


RELATED PATHWAY(S):
nucleotide excision repair (NER)
prokaryotic (SOS) response


RELATED DAMAGE:
DNA-psoralen
1,N2-alpha-methyl-gamma-OH-propano-G (mHPG)
8-oxoG
AP-site
M3dA
M1dG
CPD
B[a]P-guanine
M1A (OPA)
M2G
(6-4)PP
intErstrand crosslinks (ICLs)
intrAstrand crosslink
M1C (OPC)
M3C
OEG
p-BQ-C
p-BQ-A
p-BQ-G
aflatoxin-G


Amino acids sequence

        10         20         30         40         50         60
MSKPFKLNSA FKPSGDQPEA IRRLEEGLED GLAHQTLLGV TGSGKTFTIA NVIADLQRPT
        70         80         90        100        110        120
MVLAPNKTLA AQLYGEMKEF FPENAVEYFV SYYDYYQPEA YVPSSDTFIE KDASVNEHIE
       130        140        150        160        170        180
QMRLSATKAM LERRDVVVVA SVSAIYGLGD PDLYLKMMLH LTVGMIIDQR AILRRLAELQ
       190        200        210        220        230        240
YARNDQAFQR GTFRVRGEVI DIFPAESDDI ALRVELFDEE VERLSLFDPL TGQIVSTIPR
       250        260        270        280        290        300
FTIYPKTHYV TPRERIVQAM EEIKEELAAR RKVLLENNKL LEEQRLTQRT QFDLEMMNEL
       310        320        330        340        350        360
GYCSGIENYS RFLSGRGPGE PPPTLFDYLP ADGLLVVDES HVTIPQIGGM YRGDRARKET
       370        380        390        400        410        420
LVEYGFRLPS ALDNRPLKFE EFEALAPQTI YVSATPGNYE LEKSGGDVVD QVVRPTGLLD
       430        440        450        460        470        480
PIIEVRPVAT QVDDLLSEIR QRAAINERVL VTTLTKRMAE DLTEYLEEHG ERVRYLHSDI
       490        500        510        520        530        540
DTVERMEIIR DLRLGEFDVL VGINLLREGL DMPEVSLVAI LDADKEGFLR SERSLIQTIG
       550        560        570        580        590        600
RAARNVNGKA ILYGDKITPS MAKAIGETER RREKQQKYNE EHGITPQGLN KKVVDILALG
       610        620        630        640        650        660
QNIAKTKAKG RGKSRPIVEP DNVPMDMSPK ALQQKIHELE GLMMQHAQNL EFEEAAQIRD
       670
QLHQLRELFI AAS    

Encoded by uvrB gene


OTHER NAME(S):
dar-1
dar-6
ECK0768
JW0762


DESCRIPTION:
[More information is available at EcoCyc: EG11062].


Nucleic acid sequence

        10         20         30         40         50         60
atgagtaaac cgttcaaact gaattccgct tttaaacctt ctggcgatca gccagaggcg
        70         80         90        100        110        120
attcgacgtc tcgaagaggg gctggaagat ggcctggcgc accagacgtt acttggcgtg
       130        140        150        160        170        180
actggctcag ggaaaacctt caccattgcc aatgtcattg ctgaccttca gcgcccaacc
       190        200        210        220        230        240
atggtacttg cgcccaacaa aacgctggcg gcccagctgt atggcgaaat gaaagagttc
       250        260        270        280        290        300
ttcccggaaa acgcggtgga atatttcgtt tcctactacg actactatca gccggaagcc
       310        320        330        340        350        360
tatgtaccga gttccgacac tttcattgag aaagatgcct cggttaacga acatattgag
       370        380        390        400        410        420
cagatgcgtt tgtccgccac caaagcgatg ctggagcggc gtgatgtggt tgtggtggcg
       430        440        450        460        470        480
tctgtttccg cgatttatgg tctgggcgat cctgatttat atctcaagat gatgctccat
       490        500        510        520        530        540
ctcacggtcg gtatgattat cgatcagcgc gcgattctgc gccgactggc ggagctgcaa
       550        560        570        580        590        600
tacgctcgta atgatcaagc attccagcgt ggtactttcc gcgttcgtgg cgaggtgata
       610        620        630        640        650        660
gatatcttcc cggcagaatc ggatgacatt gcacttcgcg tggaactgtt tgacgaggaa
       670        680        690        700        710        720
gtggaacgat tgtcgttatt tgacccgctg accgggcaga ttgtttccac tattccacgt
       730        740        750        760        770        780
tttaccatct acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt acaggcgatg
       790        800        810        820        830        840
gaggagatca aagaagagct ggccgccaga cgcaaagtgc tgttggaaaa caacaaactg
       850        860        870        880        890        900
ctggaagagc agcggctgac ccagcgtacc cagtttgatc tggagatgat gaacgagctg
       910        920        930        940        950        960
ggctactgtt cggggattga aaactactcg cgcttcctct ccggtcgtgg accgggtgag
       970        980        990       1000       1010       1020
ccaccgccga cgctgtttga ttacctgcct gccgatgggc tgctggtcgt cgatgaatct
      1030       1040       1050       1060       1070       1080
cacgtcacca ttccacaaat tggcggcatg tatcgcggtg accgggcgcg taaagagaca
      1090       1100       1110       1120       1130       1140
ctggtggagt acggcttccg cctgccatca gcgctggata accgtccgct taagtttgaa
      1150       1160       1170       1180       1190       1200
gagttcgaag cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg taattacgag
      1210       1220       1230       1240       1250       1260
ctggaaaaat ccggcggcga tgtggtggat caggtggtgc gtccaaccgg attgcttgac
      1270       1280       1290       1300       1310       1320
ccgattatcg aagtgcggcc ggtggcgaca caggttgatg atcttctttc ggagattcgt
      1330       1340       1350       1360       1370       1380
cagcgagcgg caattaacga acgcgtactg gtcaccacac tgaccaagcg gatggcggaa
      1390       1400       1410       1420       1430       1440
gatcttaccg aatatctcga agaacatggc gagcgcgtgc gttatcttca ctcagatatc
      1450       1460       1470       1480       1490       1500
gacaccgtcg aacgtatgga gattatccgc gacttgcgtc tgggtgagtt cgacgtgctg
      1510       1520       1530       1540       1550       1560
gtagggatca acttactgcg cgaaggtctg gatatgccgg aagtgtcgct ggtggcgatc
      1570       1580       1590       1600       1610       1620
ctcgacgctg acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca gaccattggt
      1630       1640       1650       1660       1670       1680
cgtgcggcac gtaacgttaa cggtaaagcg attctctacg gcgataagat caccccatca
      1690       1700       1710       1720       1730       1740
atggcgaaag cgattggcga aaccgaacgt cgccgtgaga aacagcagaa gtacaacgag
      1750       1760       1770       1780       1790       1800
gaacacggaa ttacgccgca aggcttgaac aagaaagtgg tcgatatcct ggcgctgggg
      1810       1820       1830       1840       1850       1860
cagaacattg ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat tgttgagccg
      1870       1880       1890       1900       1910       1920
gataatgtgc cgatggatat gtcgcctaaa gcgttgcagc agaaaatcca tgagctggaa
      1930       1940       1950       1960       1970       1980
gggttgatga tgcaacacgc gcagaatctg gagttcgaag aagcggcgca aattcgtgac
      1990       2000       2010       2020
cagttgcatc agctgcgtga gctgtttatc gcggcatcgt aa 

Last modification date: Oct. 2, 2011