Homo sapiens L. (human) [HSA]

FULL NAME: UV excision repair protein RAD23 homolog B


DESCRIPTION:
Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmatic reticulum-associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome.
The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the XPC:RAD23B dimer is sufficient to initiate NER; it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts. XPC:RAD23B contacts DNA both 5' and 3' of a cisplatin lesion with a preference for the 5' side. XPC:RAD23B induces a bend in DNA upon binding. XPC:RAD23B stimulates the activity of DNA glycosylases TDG and SMUG1.
Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex. Cooperatively with CETN2 appears to stabilize XPC. May protect XPC from proteasomal degradation.

STRUCTURE SIMILARITY:
Contains 1 STI1 domain.
Belongs to the RAD23 family.
Contains 2 UBA domains.
Contains 1 ubiquitin-like domain.


RELATED PATHWAY(S):
nucleotide excision repair (NER)


Amino acids sequence

        10         20         30         40         50         60
MQVTLKTLQQ QTFKIDIDPE ETVKALKEKI ESEKGKDAFP VAGQKLIYAG KILNDDTALK
        70         80         90        100        110        120
EYKIDEKNFV VVMVTKPKAV STPAPATTQQ SAPASTTAVT SSTTTTVAQA PTPVPALAPT
       130        140        150        160        170        180
STPASITPAS ATASSEPAPA SAAKQEKPAE KPAETPVATS PTATDSTSGD SSRSNLFEDA
       190        200        210        220        230        240
TSALVTGQSY ENMVTEIMSM GYEREQVIAA LRASFNNPDR AVEYLLMGIP GDRESQAVVD
       250        260        270        280        290        300
PPQAASTGAP QSSAVAAAAA TTTATTTTTS SGGHPLEFLR NQPQFQQMRQ IIQQNPSLLP
       310        320        330        340        350        360
ALLQQIGREN PQLLQQISQH QEHFIQMLNE PVQEAGGQGG GGGGGSGGIA EAGSGHMNYI
       370        380        390        400
QVTPQEKEAI ERLKALGFPE GLVIQAYFAC EKNENLAANF LLQQNFDED 

Encoded by RAD23B gene

FULL NAME: RAD23 homolog B (S. cerevisiae)


OTHER NAME(S):
HHR23B
HR23B
P58


DESCRIPTION:
The protein encoded by this gene is one of two human homologs of Saccharomyces cerevisiae Rad23, a protein involved in the nucleotide excision repair (NER). This protein was found to be a component of the protein complex that specifically complements the NER defect of xeroderma pigmentosum group C (XP-c) cell extracts in vitro. This protein was also shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, and thus this protein may be involved in the ubiquitin mediated proteolytic pathway in cells. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2011]


Nucleic acid sequence

        10         20         30         40         50         60
atgcaggtca ccctgaagac cctccagcag cagaccttca agatagacat tgaccccgag
        70         80         90        100        110        120
gagacggtga aagcactgaa agagaagatt gaatctgaaa aggggaaaga tgcctttcca
       130        140        150        160        170        180
gtagcaggtc aaaaattaat ttatgcaggc aaaatcctca atgatgatac tgctctcaaa
       190        200        210        220        230        240
gaatataaaa ttgatgagaa aaactttgtg gtggttatgg tgaccaaacc caaagcagtg
       250        260        270        280        290        300
tccacaccag caccagctac aactcagcag tcagctcctg ccagcactac agcagttact
       310        320        330        340        350        360
tcctccacca ccacaactgt ggctcaggct ccaacccctg tccctgcctt ggcccccact
       370        380        390        400        410        420
tccacacctg catccatcac tccagcatca gcgacagcat cttctgaacc tgcacctgct
       430        440        450        460        470        480
agtgcagcta aacaagagaa gcctgcagaa aagccagcag agacaccagt ggctactagc
       490        500        510        520        530        540
ccaacagcaa ctgacagtac atcgggtgat tcttctcggt caaacctttt tgaagatgca
       550        560        570        580        590        600
acgagtgcac ttgtgacggg tcagtcttac gagaatatgg taactgagat catgtcaatg
       610        620        630        640        650        660
ggctatgaac gagagcaagt aattgcagcc ctgagagcca gtttcaacaa ccctgacaga
       670        680        690        700        710        720
gcagtggagt atcttttaat gggaatccct ggagatagag aaagtcaggc tgtggttgac
       730        740        750        760        770        780
ccccctcaag cagctagtac tggggctcct cagtcttcag cagtggctgc agctgcagca
       790        800        810        820        830        840
actacgacag caacaactac aacaacaagt tctggaggac atccccttga atttttacgg
       850        860        870        880        890        900
aatcagcctc agtttcaaca gatgagacaa attattcagc agaatccttc cttgcttcca
       910        920        930        940        950        960
gcgttactac agcagatagg tcgagagaat cctcaattac ttcagcaaat tagccaacac
       970        980        990       1000       1010       1020
caggagcatt ttattcagat gttaaatgaa ccagttcaag aagctggtgg tcaaggagga
      1030       1040       1050       1060       1070       1080
ggaggtggag gtggcagtgg aggaattgca gaagctggaa gtggtcatat gaactacatt
      1090       1100       1110       1120       1130       1140
caagtaacac ctcaggaaaa agaagctata gaaaggttaa aggcattagg atttcctgaa
      1150       1160       1170       1180       1190       1200
ggacttgtga tacaagcgta ttttgcttgt gagaagaatg agaatttggc tgccaatttt
      1210       1220       1230
cttctacagc agaactttga tgaagattga   

Last modification date: Oct. 2, 2011