Mus musculus L. (mouse) [MMU]

FULL NAME: DNA polymerase beta


DESCRIPTION:
Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases (By similarity).

STRUCTURE SIMILARITY:
Belongs to the DNA polymerase type-X family.


CATALYTIC ACTIVITY:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).


POST-TRANSLATIONAL MODIFICATION:
Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity (By similarity).
Ubiquitinated at Lys-41, Lys-61 and Lys-81: monoubiquitinated by HUWE1/ARF-BP1. Monoubiquitinated protein is then the target of STUB1/CHIP, which catalyzes polyubiquitination from monoubiquitin, leading to degradation by the proteasome. USP47 mediates the deubiquitination of monoubiquitinated protein, preventing polyubiquitination by STUB1/CHIP and its subsequent degradation (By similarity).


PROTEIN TYPE(S):
X-family DNA polymerase
dRP-lyase


RELATED PATHWAY(S):
base excision repair (BER)
translesion synthesis (TLS)


Amino acids sequence

        10         20         30         40         50         60
MSKRKAPQET LNGGITDMLV ELANFEKNVS QAIHKYNAYR KAASVIAKYP HKIKSGAEAK
        70         80         90        100        110        120
KLPGVGTKIA EKIDEFLATG KLRKLEKIRQ DDTSSSINFL TRVTGIGPSA ARKFVDEGIK
       130        140        150        160        170        180
TLEDLRKNED KLNHHQRIGL KYFEDFEKRI PREEMLQMQD IVLNEIKKVD SEYIATVCGS
       190        200        210        220        230        240
FRRGAESSGD MDVLLTHPNF TSESSKQPKL LHRVVEQLQK VHFITDTLSK GETKFMGVCQ
       250        260        270        280        290        300
LPSEKDGKEY PHRRIDIRLI PKDQYYCGVL YFTGSDIFNK NMRAHALEKG FTINEYTIRP
       310        320        330
LGVTGVAGEP LPVDSEQDIF DYIQWRYREP KDRSE  

Encoded by Polb gene

FULL NAME: polymerase (DNA directed), beta


OTHER NAME(S):
A430088C08Rik


Nucleic acid sequence

        10         20         30         40         50         60
atgagcaaac gcaaggcgcc gcaggagacc ctcaacggcg gcatcacgga catgctcgtg
        70         80         90        100        110        120
gaactcgcaa actttgagaa gaacgtgagc caggcgatcc acaagtacaa tgcgtacaga
       130        140        150        160        170        180
aaagcggcat ctgtgatagc caagtaccca cacaaaatca agagcggagc ggaagctaag
       190        200        210        220        230        240
aaactgccag gagtaggaac aaaaattgct gaaaagattg atgaattttt agcaactgga
       250        260        270        280        290        300
aaattgcgta aactggaaaa gattcgtcaa gatgatacaa gttcatccat caatttcctg
       310        320        330        340        350        360
actcgagtta ctggcattgg accatctgct gcaaggaagt ttgtagatga aggaattaag
       370        380        390        400        410        420
acattagaag atctcaggaa aaatgaagat aaactgaacc atcatcaacg aattgggctg
       430        440        450        460        470        480
aaatatttcg aggactttga aaagagaatt cctcgtgagg aaatgctgca gatgcaggat
       490        500        510        520        530        540
attgtactta atgaaattaa aaaagtggac tctgagtaca ttgctacagt ctgtggcagt
       550        560        570        580        590        600
ttcagaagag gcgcagagtc gagtggagac atggatgtcc tgctgaccca cccaaacttc
       610        620        630        640        650        660
acgtcagaat ccagcaaaca gccaaagttg ttacatcgtg ttgtggaaca gttacaaaaa
       670        680        690        700        710        720
gtccatttca ttacagatac tctgtcaaag ggtgaaacaa agttcatggg tgtttgccag
       730        740        750        760        770        780
cttcccagcg agaaggatgg aaaggaatat ccacacagga gaatcgatat caggttgatc
       790        800        810        820        830        840
cccaaagatc agtactactg tggtgttctc tacttcactg ggagtgacat ctttaataag
       850        860        870        880        890        900
aacatgagag cgcacgccct ggaaaagggc ttcacaatca atgagtacac catccgcccc
       910        920        930        940        950        960
ctgggggtca ctggagtcgc tggggagccc cttcctgtgg acagtgagca ggacattttt
       970        980        990       1000
gattacatcc agtggcgcta ccgggagccc aaggatagaa gtgaatga 

Last modification date: Oct. 2, 2011