Schizo-saccharomyces pombe Lindner (fission yeast) [SPO]

FULL NAME: Serine/threonine-protein kinase chk1


DESCRIPTION:
Involved in cell cycle arrest when DNA damage has occurred or when unligated DNA is present. Binds to and phosphorylates CDC25. This leads to negative regulation of CDC25 and prevents mitotic entry.

STRUCTURE SIMILARITY:
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.
Contains 1 protein kinase domain.


CATALYTIC ACTIVITY:
ATP + a protein = ADP + a phosphoprotein.


POST-TRANSLATIONAL MODIFICATION:
Phosphorylated.


RELATED PATHWAY(S):
DNA damage response (DDR)


Amino acids sequence

        10         20         30         40         50         60
MAQKLDNFPY HIGREIGTGA FASVRLCYDD NAKIYAVKFV NKKHATSCMN AGVWARRMAS
        70         80         90        100        110        120
EIQLHKLCNG HKNIIHFYNT AENPQWRWVV LEFAQGGDLF DKIEPDVGID EDVAQFYFAQ
       130        140        150        160        170        180
LMEGISFMHS KGVAHRDLKP ENILLDYNGN LKISDFGFAS LFSYKGKSRL LNSPVGSPPY
       190        200        210        220        230        240
AAPEITQQYD GSKVDVWSCG IILFALLLGN TPWDEAISNT GDYLLYKKQC ERPSYHPWNL
       250        260        270        280        290        300
LSPGAYSIIT GMLRSDPFKR YSVKHVVQHP WLTSSTPFRT KNGNCADPVA LASRLMLKLR
       310        320        330        340        350        360
IDLDKPRLAS SRASQNDSGF SMTQPAFKKN DQKELDRVEV YGALSQPVQL NKNIDVTEIL
       370        380        390        400        410        420
EKDPSLSQFC ENEGFIKRLA KKAKNFYEIC PPERLTRFYS RASRETIIDH LYDSLRLLAI
       430        440        450        460        470        480
SVTMKYVRNQ TILYVNLHDK RKCLLQGVIE LTNLGHNLEL INFIKRNGDP LEWRKFFKNV
       490
VSSIGKPIVL TDVSQN    

Encoded by chk1 gene


Nucleic acid sequence

        10         20         30         40         50         60
atggctcaaa aattagataa ctttccttac catattggta gagaaatagg tactggggct
        70         80         90        100        110        120
tttgcttccg tccgtttatg ttacgatgat aatgctaaaa tatatgctgt caaatttgtc
       130        140        150        160        170        180
aataaaaaac atgctactag ttgtatgaat gcaggggtat gggctagaag gatggctagt
       190        200        210        220        230        240
gaaatacaac ttcacaaact atgcaatgga cataaaaata tcattcattt ttataataca
       250        260        270        280        290        300
gcagaaaatc cccaatggag atgggtagta cttgaatttg ctcaaggtgg tgacttattt
       310        320        330        340        350        360
gacaaaatag agcctgatgt aggtattgat gaggacgtcg ctcagtttta ttttgctcaa
       370        380        390        400        410        420
cttatggaag gtatttcctt catgcactct aaaggtgtcg cgcatcgaga cttgaaacct
       430        440        450        460        470        480
gagaacatcc ttttagatta caatggtaac ttaaagattt cagacttcgg ctttgcatct
       490        500        510        520        530        540
ttattttcct ataaaggtaa aagtaggctt ttgaatagtc cagtgggtag tccaccatac
       550        560        570        580        590        600
gctgctccag aaattacaca gcagtatgat ggttcaaaag ttgatgtctg gtcctgcggt
       610        620        630        640        650        660
ataattcttt ttgctttgtt attaggcaac acaccttggg atgaagcaat tagcaacact
       670        680        690        700        710        720
ggtgactact tgctttataa aaaacaatgt gaacgcccgt cttaccaccc atggaacttg
       730        740        750        760        770        780
ttgtctccag gagcctattc gattatcacc ggaatgcttc gaagtgatcc atttaagcgc
       790        800        810        820        830        840
tactctgtca aacatgttgt acagcaccca tggcttacct caagtacacc atttcgaact
       850        860        870        880        890        900
aaaaatggga attgtgccga tcctgtagca ttggcatcaa ggctcatgtt gaaacttcgt
       910        920        930        940        950        960
attgatttag ataaaccacg tcttgcctca tctagagcat ctcaaaacga tagcggtttt
       970        980        990       1000       1010       1020
tcaatgacac agccagcttt taaaaaaaat gaccaaaagg agttggatcg tgtagaagtt
      1030       1040       1050       1060       1070       1080
tatggcgcct tatcccagcc agtacaactt aataaaaata ttgacgttac tgaaatcctt
      1090       1100       1110       1120       1130       1140
gaaaaagacc cctcattgag tcagttttgt gagaatgaag gttttattaa aagattggcc
      1150       1160       1170       1180       1190       1200
aaaaaggcta agaatttcta cgaaatttgt cctcctgaaa gactcactag gttttattct
      1210       1220       1230       1240       1250       1260
agggcgtcga gagaaaccat tatagatcat ctttatgaca gtctacgact acttgcaatc
      1270       1280       1290       1300       1310       1320
tcagtgacta tgaaatatgt tcgaaatcag accatccttt atgttaattt acatgataaa
      1330       1340       1350       1360       1370       1380
cggaaatgtc tcttgcaagg tgttattgaa ctaactaatt taggccataa cttagagctt
      1390       1400       1410       1420       1430       1440
ataaacttta taaagcgtaa tggggatcct cttgaatgga gaaaattttt taaaaacgtt
      1450       1460       1470       1480       1490
gtcagttcaa tagggaagcc gattgttctt acagatgttt cacaaaatta a

Last modification date: Oct. 2, 2011