Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: Serine/threonine-protein kinase CHK1


DESCRIPTION:
Involved in cell cycle arrest when DNA damage has occurred or when unligated DNA is present. Controls phosphorylation and abundance of PDS1 to prevent anaphase entry. Also helps prevent mitotic exit.

MISCELLANEOUS:
Present with 2530 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. NIM1 subfamily.
Contains 1 protein kinase domain.


CATALYTIC ACTIVITY:
ATP + a protein = ADP + a phosphoprotein.


RELATED PATHWAY(S):
DNA damage response (DDR)


Amino acids sequence

        10         20         30         40         50         60
MSLSQVSPLP HIKDVVLGDT VGQGAFACVK NAHLQMDPSI ILAVKFIHVP TCKKMGLSDK
        70         80         90        100        110        120
DITKEVVLQS KCSKHPNVLR LIDCNVSKEY MWIILEMADG GDLFDKIEPD VGVDSDVAQF
       130        140        150        160        170        180
YFQQLVSAIN YLHVECGVAH RDIKPENILL DKNGNLKLAD FGLASQFRRK DGTLRVSMDQ
       190        200        210        220        230        240
RGSPPYMAPE VLYSEEGYYA DRTDIWSIGI LLFVLLTGQT PWELPSLENE DFVFFIENDG
       250        260        270        280        290        300
NLNWGPWSKI EFTHLNLLRK ILQPDPNKRV TLKALKLHPW VLRRASFSGD DGLCNDPELL
       310        320        330        340        350        360
AKKLFSHLKV SLSNENYLKF TQDTNSNNRY ISTQPIGNEL AELEHDSMHF QTVSNTQRAF
       370        380        390        400        410        420
TSYDSNTNYN SGTGMTQEAK WTQFISYDIA ALQFHSDEND CNELVKRHLQ FNPNKLTKFY
       430        440        450        460        470        480
TLQPMDVLLP ILEKALNLSQ IRVKPDLFAN FERLCELLGY DNVFPLIINI KTKSNGGYQL
       490        500        510        520
CGSISIIKIE EELKSVGFER KTGDPLEWRR LFKKISTICR DIILIPN 

Encoded by CHK1 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgagtctct cgcaggtgtc acctttaccc catatcaagg atgttgtttt aggagataca
        70         80         90        100        110        120
gtaggccaag gcgcattcgc ctgcgttaaa aatgctcatc ttcaaatgga tccctccatt
       130        140        150        160        170        180
attctagctg ttaaattcat tcatgttccc acttgcaaaa aaatgggact cagtgacaag
       190        200        210        220        230        240
gatatcacaa aagaggttgt tttgcaatcg aagtgttcta agcatcctaa tgttttgaga
       250        260        270        280        290        300
cttatcgatt gtaatgtctc taaagaatat atgtggataa ttctggagat ggcagatggt
       310        320        330        340        350        360
ggtgatctat ttgataagat tgagcctgac gttggagttg attccgacgt ggcccaattt
       370        380        390        400        410        420
tactttcaac agctcgttag tgctattaat tatctgcatg tagaatgtgg agttgctcac
       430        440        450        460        470        480
agagacatca agcctgaaaa catcttactc gataagaacg gaaatctgaa gctagctgat
       490        500        510        520        530        540
tttgggctcg cctctcaatt taggaggaaa gatggtacat tacgtgtatc catggatcaa
       550        560        570        580        590        600
aggggttctc caccctacat ggctcctgaa gtattatatt ctgaagaggg ttattacgca
       610        620        630        640        650        660
gatcgaacag atatatggtc tattggcatc ctcttgttcg tattgttgac tggtcaaacg
       670        680        690        700        710        720
ccttgggaat taccttcatt agagaacgaa gatttcgtct tttttattga aaatgatgga
       730        740        750        760        770        780
aatttaaact ggggaccctg gtcaaagata gaatttactc acttgaacct actccgaaaa
       790        800        810        820        830        840
attttacaac ctgacccaaa taagagggtg acattgaagg ctttaaagtt acatccttgg
       850        860        870        880        890        900
gtattacgtc gagcttcatt ttctggggat gatggtctct gtaatgaccc tgaactcttg
       910        920        930        940        950        960
gctaagaaac tgttttctca cttaaaagtc tcactgagta acgaaaatta tttgaaattc
       970        980        990       1000       1010       1020
actcaagata caaactctaa taataggtac atttctactc agccaattgg taacgaattg
      1030       1040       1050       1060       1070       1080
gctgagcttg aacacgactc aatgcatttc cagacagttt cgaatacaca acgtgcattt
      1090       1100       1110       1120       1130       1140
acctcgtatg attcaaatac gaactataat agcggaacag gtatgacaca agaggctaag
      1150       1160       1170       1180       1190       1200
tggacgcaat tcataagcta tgatatcgct gccttacagt ttcattctga tgaaaatgat
      1210       1220       1230       1240       1250       1260
tgtaatgaat tagtaaaacg acatttacaa tttaatccga ataaacttac caagttctac
      1270       1280       1290       1300       1310       1320
acgttgcaac ctatggatgt tttgttaccg attttggaga aagccttgaa tttatcacaa
      1330       1340       1350       1360       1370       1380
attagagtaa aacccgacct ttttgcgaat tttgaaagat tgtgcgaatt attgggttat
      1390       1400       1410       1420       1430       1440
gataacgttt tcccacttat tataaatatt aaaaccaaaa gtaatggggg ttatcaatta
      1450       1460       1470       1480       1490       1500
tgcggtagca tttccatcat aaagattgaa gaagagttga aaagtgtcgg atttgaaaga
      1510       1520       1530       1540       1550       1560
aaaactggtg atcctttaga atggagaaga ttgttcaaga aaatttcaac tatctgtagg
      1570       1580
gatattatcc taattcccaa ctga   

Last modification date: Oct. 2, 2011