Mus musculus L. (mouse) [MMU]

FULL NAME: G/T mismatch-specific thymine DNA glycosylase


DESCRIPTION:
In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.

STRUCTURE SIMILARITY:
Belongs to the TDG/mug DNA glycosylase family.


CATALYTIC ACTIVITY:
Hydrolyzes mismatched double-stranded DNA and polynucleotides, releasing free thymine.


POST-TRANSLATIONAL MODIFICATION:
Sumoylation on Lys-317 by either SUMO1 or SUMO2 induces dissociation of the product DNA (By similarity).


PROTEIN TYPE(S):
DNA N-glycosylase


RELATED PATHWAY(S):
base excision repair (BER)


Amino acids sequence

        10         20         30         40         50         60
MMAEVPNMAV TTGQQVPAVA PNMATVTEQQ VPEDAPVQEP APEAPKRRKR KPRAAEPQEP
        70         80         90        100        110        120
VEPKKPATSK KSGKSTKSKE KQEKITDAFK VKRKVDRFNG VSEAELLTKT LPDILTFNLD
       130        140        150        160        170        180
IVIIGINPGL MAAYKGHHYP GPGNHFWKCL FMSGLSEVQL NHMDDHTLPG KYGIGFTNMV
       190        200        210        220        230        240
ERTTPGSKDL SSKEFREGGR ILVQKLQKYQ PRIAVFNGKC IYEIFSKEVF GVKVKNLEFG
       250        260        270        280        290        300
LQPHKIPDTE TLCYVMPSSS ARCAQFPRAQ DKVHYYIKLK DLRDQLKGIE RNADVQEVQY
       310        320        330        340        350        360
TFDLQLAQED AKKMAVKEEK YDPGYEAAYG GAYGENPCNG EPCGIASNGL TAHSAEPRGE
       370        380        390
AAPSDVPNGQ WMAQSFAEQI PSFNNCGTRE QEEESHA  

Encoded by Tdg gene

FULL NAME: thymine DNA glycosylase


OTHER NAME(S):
E130317C12Rik
JZA-3
Jza1


Nucleic acid sequence

        10         20         30         40         50         60
atgatggcag aagttcctaa catggcagtc acgactggac agcaggtgcc agcagtagct
        70         80         90        100        110        120
cctaacatgg caaccgtgac agaacagcag gtgcccgaag acgctcctgt ccaggaacct
       130        140        150        160        170        180
gcaccagaag ctccaaagag aaggaaaagg aaacccagag cagcagagcc ccaggaacca
       190        200        210        220        230        240
gtggagccca aaaaacctgc tacgtcgaag aaatccggca agtctacaaa atcaaaggaa
       250        260        270        280        290        300
aagcaggaga aaatcacaga cgcgtttaaa gtgaaaagga aagtggaccg cttcaacggc
       310        320        330        340        350        360
gtctctgaag ctgagcttct gaccaagact cttcctgaca ttttgacctt caatctggat
       370        380        390        400        410        420
attgtgatca ttggcattaa cccgggatta atggctgctt acaaaggaca tcactaccct
       430        440        450        460        470        480
gggcctggaa atcacttctg gaagtgtctg ttcatgtcgg ggctgagtga ggtgcagctg
       490        500        510        520        530        540
aatcacatgg atgaccacac cttacccggc aagtacggca tcggattcac caacatggtg
       550        560        570        580        590        600
gaacggacga cgccgggcag caaggatctg tctagtaaag agttccggga aggagggcgc
       610        620        630        640        650        660
atcctggtgc agaaactgca gaaatatcag ccacgaatag cggtgtttaa tggaaaatgt
       670        680        690        700        710        720
atttatgaaa ttttcagtaa agaagttttt ggagtaaagg ttaagaactt ggaatttggg
       730        740        750        760        770        780
cttcaacccc acaagatccc agacacagaa actctgtgct acgtcatgcc gtcgtccagc
       790        800        810        820        830        840
gccagatgtg ctcagtttcc ccgggcccag gacaaagttc attactacat taagctgaag
       850        860        870        880        890        900
gacttgagag accaactgaa aggcattgaa cgcaacgcgg acgttcagga agtgcagtat
       910        920        930        940        950        960
acatttgacc tgcagcttgc gcaagaggac gcaaagaaga tggctgttaa ggaagaaaag
       970        980        990       1000       1010       1020
tatgatccag gctatgaggc agcttacggc ggtgcctatg gggaaaaccc atgtaatggg
      1030       1040       1050       1060       1070       1080
gaaccttgtg gcattgcttc aaatgggcta acagctcaca gtgcggagcc gagaggagaa
      1090       1100       1110       1120       1130       1140
gcggccccca gcgatgttcc gaatgggcag tggatggcac agtcgtttgc agagcagatc
      1150       1160       1170       1180       1190
ccttctttta ataattgtgg gacccgagag caggaagaag agagccacgc ttag

Last modification date: Oct. 2, 2011