Homo sapiens L. (human) [HSA]

FULL NAME: Structure-specific endonuclease subunit SLX4


DESCRIPTION:
Regulatory subunit that interacts with and increases the activity of different structure-specific endonucleases. Has several distinct roles in protecting genome stability by resolving diverse forms of deleterious DNA structures originating from replication and recombination intermediates and from DNA damage. Component of the SLX1-SLX4 structure-specific endonuclease that resolves DNA secondary structures generated during DNA repair and recombination. Has endonuclease activity towards branched DNA substrates, introducing single-strand cuts in duplex DNA close to junctions with ss-DNA. Has a preference for 5'-flap structures, and promotes symmetrical cleavage of static and migrating Holliday junctions (HJs). Resolves HJs by generating two pairs of ligatable, nicked duplex products. Interacts with the structure-specific ERCC4-ERCC1 endonuclease and promotes the cleavage of bubble structures. Interacts with the structure-specific MUS81-EME1 endonuclease and promotes the cleavage of 3'-flap and replication fork-like structures. SLX4 is required for recovery from alkylation-induced DNA damage and is involved in the resolution of DNA double-strand breaks.

STRUCTURE SIMILARITY:
Belongs to the SLX4 family.
Contains 1 BTB (POZ) domain.


POST-TRANSLATIONAL MODIFICATION:
Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity).


RELATED PATHWAY(S):
Fanconi anemia (FA) pathway


RELATED DAMAGE:
intErstrand crosslinks (ICLs)


Amino acids sequence

        10         20         30         40         50         60
MKLSVNEAQL GFYLGSLSHL SACPGIDPRS SEDQPESLKT GQMMDESDED FKELCASFFQ
        70         80         90        100        110        120
RVKKHGIKEV SGERKTQKAA SNGTQIRSKL KRTKQTATKT KTLQGPAEKK PPSGSQAPRT
       130        140        150        160        170        180
KKQRVTKWQA SEPAHSVNGE GGVLASAPDP PVLRETAQNT QTGNQQEPSP NLSREKTREN
       190        200        210        220        230        240
VPNSDSQPPP SCLTTAVPSP SKPRTAQLVL QRMQQFKRAD PERLRHASEE CSLEAAREEN
       250        260        270        280        290        300
VPKDPQEEMM AGNVYGLGPP APESDAAVAL TLQQEFARVG ASAHDDSLEE KGLFFCQICQ
       310        320        330        340        350        360
KNLSAMNVTR REQHVNRCLD EAEKTLRPSV PQIPECPICG KPFLTLKSRT SHLKQCAVKM
       370        380        390        400        410        420
EVGPQLLLQA VRLQTAQPEG SSSPPMFSFS DHSRGLKRRG PTSKKEPRKR RKVDEAPSED
       430        440        450        460        470        480
LLVAMALSRS EMEPGAAVPA LRLESAFSER IRPEAENKSR KKKPPVSPPL LLVQDSETTG
       490        500        510        520        530        540
RQIEDRVALL LSEEVELSST PPLPASRILK EGWERAGQCP PPPERKQSFL WEGSALTGAW
       550        560        570        580        590        600
AMEDFYTARL VPPLVPQRPA QGLMQEPVPP LVPPEHSELS ERRSPALHGT PTAGCGSRGP
       610        620        630        640        650        660
SPSASQREHQ ALQDLVDLAR EGLSASPWPG SGGLAGSEGT AGLDVVPGGL PLTGFVVPSQ
       670        680        690        700        710        720
DKHPDRGGRT LLSLGLLVAD FGAMVNNPHL SDVQFQTDSG EVLYAHKFVL YARCPLLIQY
       730        740        750        760        770        780
VNNEGFSAVE DGVLTQRVLL GDVSTEAART FLHYLYTADT GLPPGLSSEL SSLAHRFGVS
       790        800        810        820        830        840
ELVHLCEQVP IATDSEGKPW EEKEAENCES RAENFQELLR SMWADEEEEA ETLLKSKDHE
       850        860        870        880        890        900
EDQENVNEAE MEEIYEFAAT QRKLLQEERA AGAGEDADWL EGGSPVSGQL LAGVQVQKQW
       910        920        930        940        950        960
DKVEEMEPLE PGRDEAATTW EKMGQCALPP PQGQHSGARG AEAPEQEAPE EALGHSSCSS
       970        980        990       1000       1010       1020
PSRDCQAERK EGSLPHSDDA GDYEQLFSST QGEISEPSQI TSEPEEQSGA VRERGLEVSH
      1030       1040       1050       1060       1070       1080
RLAPWQASPP HPCRFLLGPP QGGSPRGSHH TSGSSLSTPR SRGGTSQVGS PTLLSPAVPS
      1090       1100       1110       1120       1130       1140
KQKRDRSILT LSKEPGHQKG KERRSVLECR NKGVLMFPEK SPSIDLTQSN PDHSSSRSQK
      1150       1160       1170       1180       1190       1200
SSSKLNEEDE VILLLDSDEE LELEQTKMKS ISSDPLEEKK ALEISPRSCE LFSIIDVDAD
      1210       1220       1230       1240       1250       1260
QEPSQSPPRS EAVLQQEDEG ALPENRGSLG RRGAPWLFCD RESSPSEAST TDTSWLVPAT
      1270       1280       1290       1300       1310       1320
PLASRSRDCS SQTQISSLRS GLAVQAVTQH TPRASVGNRE GNEVAQKFSV IRPQTPPPQT
      1330       1340       1350       1360       1370       1380
PSSCLTPVSP GTSDGRRQGH RSPSRPHPGG HPHSSPLAPH PISGDRAHFS RRFLKHSPPG
      1390       1400       1410       1420       1430       1440
PSFLNQTPAG EVVEVGDSDD EQEVASHQAN RSPPLDSDPP IPIDDCCWHM EPLSPIPIDH
      1450       1460       1470       1480       1490       1500
WNLERTGPLS TSSPSRRMNE AADSRDCRSP GLLDTTPIRG SCTTQRKLQE KSSGAGSLGN
      1510       1520       1530       1540       1550       1560
SRPSFLNSAL WDVWDGEEQR PPETPPPAQM PSAGGAQKPE GLETPKGANR KKNLPPKVPI
      1570       1580       1590       1600       1610       1620
TPMPQYSIME TPVLKKELDR FGVRPLPKRQ MVLKLKEIFQ YTHQTLDSDS EDESQSSQPL
      1630       1640       1650       1660       1670       1680
LQAPHCQTLA SQTYKPSRAG VHAQQEATTG PGAHRPKGPA KTKGPRHQRK HHESITPPSR
      1690       1700       1710       1720       1730       1740
SPTKEAPPGL NDDAQIPASQ ESVATSVDGS DSSLSSQSSS SCEFGAAFES AGEEEGEGEV
      1750       1760       1770       1780       1790       1800
SASQAAVQAA DTDEALRCYI RSKPALYQKV LLYQPFELRE LQAELRQNGL RVSSRRLLDF
      1810       1820       1830
LDTHCITFTT AATRREKLQG RRRQPRGKKK VERN  

Encoded by SLX4 gene

FULL NAME: SLX4 structure-specific endonuclease subunit homolog (S. cerevisiae)


OTHER NAME(S):
BTBD12
FANCP
KIAA1784
KIAA1987
MUS312


DESCRIPTION:
This gene encodes a structure-specific endonuclease subunit. The encoded protein contains a central BTB domain and it forms a multiprotein complex with the ERCC4(XPF)-ERCC1, MUS81-EME1, and SLX1 endonucleases, and also associates with MSH2/MSH3 mismatch repair complex, telomere binding complex TERF2(TRF2)-TERF2IP(RAP1), the protein kinase PLK1 and the uncharacterized protein C20orf94. The multiprotein complex is required for repair of specific types of DNA lesions and is critical for cellular responses to replication fork failure. The encoded protein acts as a docking platform for the assembly of multiple structure-specific endonucleases.[provided by RefSeq, Jan 2011]


Nucleic acid sequence

        10         20         30         40         50         60
atgaaactga gtgtgaatga ggctcagcta ggcttctact tgggttcact ttctcatctg
        70         80         90        100        110        120
tctgcctgtc ctgggattga ccctcgctcc tctgaagacc agcctgaaag ccttaaaact
       130        140        150        160        170        180
ggtcagatga tggatgagtc tgatgaggac tttaaagaac tctgcgctag ctttttccaa
       190        200        210        220        230        240
agggtgaaaa aacatggaat caaggaagtg tcaggagaaa ggaagacaca aaaggctgcc
       250        260        270        280        290        300
tcaaacggca ctcagataag aagcaaattg aaaaggacca aacaaactgc taccaagacc
       310        320        330        340        350        360
aaaacccttc aaggccctgc agagaagaaa cctccgtctg gcagccaggc ccctaggact
       370        380        390        400        410        420
aaaaagcaaa gggtaaccaa atggcaagca agtgaaccgg cccactctgt gaatggggag
       430        440        450        460        470        480
gggggtgtgc ttgcctctgc tccagatcca cctgtgctcc gggaaacagc acaaaacacc
       490        500        510        520        530        540
cagacgggta accagcaaga accatcgcca aacctttcca gagagaaaac cagagagaat
       550        560        570        580        590        600
gtgcccaaca gcgactccca gcctcctcct tcctgtttga caacagcagt gccaagtccc
       610        620        630        640        650        660
tccaaacccc gcacagcaca attggtccta cagcgaatgc agcagttcaa gagagcagac
       670        680        690        700        710        720
cccgagcgtt tgagacacgc ttcagaagag tgctccctcg aggctgcgcg ggaagaaaat
       730        740        750        760        770        780
gtcccaaagg atcctcaaga ggagatgatg gcggggaatg tgtatgggct tgggccccct
       790        800        810        820        830        840
gccccagaga gcgacgctgc ggtggccttg accctgcagc aggagtttgc acgggtagga
       850        860        870        880        890        900
gcatcggcac atgatgatag cctggaggaa aagggtttgt tcttctgcca gatttgtcaa
       910        920        930        940        950        960
aagaacctct cagccatgaa cgtgacccga agggaacagc atgtgaacag gtgcttggat
       970        980        990       1000       1010       1020
gaagctgaaa agacactaag accttctgtg cctcagatcc ctgagtgccc gatttgtggg
      1030       1040       1050       1060       1070       1080
aaaccgtttc ttaccttaaa gagcagaacc agtcacttga agcagtgtgc tgtgaagatg
      1090       1100       1110       1120       1130       1140
gaggttggcc cccagctcct gcttcaggct gtgcggctgc agacagcaca gcctgagggt
      1150       1160       1170       1180       1190       1200
agcagcagcc cacccatgtt cagcttcagt gatcacagta gaggtctgaa acggagagga
      1210       1220       1230       1240       1250       1260
cccaccagca agaaggagcc acggaagagg cggaaggtgg acgaggcacc gtccgaggac
      1270       1280       1290       1300       1310       1320
ctgctggtgg ccatggctct gtcccggtcg gagatggagc cgggtgcggc tgtaccagcg
      1330       1340       1350       1360       1370       1380
ctcaggctgg aaagtgcctt ttctgagagg ataagaccag aagcagagaa taaaagtcgc
      1390       1400       1410       1420       1430       1440
aagaagaaac ccccggtatc ccccccattg ttgttagtcc aggactctga aaccacaggc
      1450       1460       1470       1480       1490       1500
cgacagatag aggaccgtgt ggccctgctc ctctctgagg aagtggaatt gtctagcacg
      1510       1520       1530       1540       1550       1560
ccaccacttc ctgccagcag gattttaaag gaagggtggg aaagagcggg ccagtgtcct
      1570       1580       1590       1600       1610       1620
cctccacctg aacgcaagca gagctttctg tgggagggca gcgcactgac tggggcctgg
      1630       1640       1650       1660       1670       1680
gccatggagg acttctacac ggccaggctg gtccctcctc tcgtgcccca gcggcctgcc
      1690       1700       1710       1720       1730       1740
cagggcctta tgcaggagcc cgtgccgcct ctggtgccac ctgagcactc agagctgagc
      1750       1760       1770       1780       1790       1800
gagcgaaggt cacccgctct ccacggcacc cccactgcag gctgtggctc caggggcccg
      1810       1820       1830       1840       1850       1860
tcgccttcgg ccagccagag ggagcaccag gccctgcagg acctcgtgga cctggcgagg
      1870       1880       1890       1900       1910       1920
gagggactga gcgccagccc gtggcccggc agtgggggcc tggctggctc ggaagggact
      1930       1940       1950       1960       1970       1980
gcagggttgg acgtggtgcc cggcggcctt cctctgactg ggtttgtggt gccatcgcag
      1990       2000       2010       2020       2030       2040
gacaagcacc cggacagggg cggccgcacc ttgctctccc tcgggctgct ggttgctgac
      2050       2060       2070       2080       2090       2100
tttggcgcca tggtcaataa cccacacctg agtgatgtcc agtttcagac ggacagcggg
      2110       2120       2130       2140       2150       2160
gaggtgcttt acgcccacaa gttcgtgctt tatgcccgat gcccgctcct catccagtat
      2170       2180       2190       2200       2210       2220
gtgaacaatg aaggcttctc cgctgtagag gacggggttc tgacccagcg tgtcctgctg
      2230       2240       2250       2260       2270       2280
ggtgacgtga gcaccgaggc cgcccgcacg ttcctgcact atctctacac tgcggacact
      2290       2300       2310       2320       2330       2340
ggccttcctc ctggccttag ctctgagctg agctccctgg cccacaggtt tggcgtgagt
      2350       2360       2370       2380       2390       2400
gagctcgttc acctgtgcga acaggtgcct attgccactg actcagaggg caaaccatgg
      2410       2420       2430       2440       2450       2460
gaggagaagg aagcagagaa ttgcgaaagc agggccgaga atttccagga actcttgagg
      2470       2480       2490       2500       2510       2520
tcaatgtggg cagatgaaga ggaggaagcg gagactttgt tgaaatccaa ggaccacgaa
      2530       2540       2550       2560       2570       2580
gaagatcaag aaaacgtgaa tgaagcagaa atggaagaaa tttatgaatt tgcagctact
      2590       2600       2610       2620       2630       2640
cagcgaaagc ttctccagga agaaagggca gcgggtgccg gcgaggacgc tgactggctg
      2650       2660       2670       2680       2690       2700
gagggtggca gtccggtttc tgggcaactc ctagcaggtg tccaggtgca gaaacagtgg
      2710       2720       2730       2740       2750       2760
gacaaggtgg aggagatgga gccgttggag ccaggaagag atgaggccgc caccacctgg
      2770       2780       2790       2800       2810       2820
gagaagatgg gacagtgcgc tctcccgcca ccccagggcc agcactcagg ggcacgggga
      2830       2840       2850       2860       2870       2880
gcagaggccc ctgagcagga ggcgccagag gaggcgcttg gccattccag ctgctccagc
      2890       2900       2910       2920       2930       2940
ccttccaggg actgccaggc agagagaaaa gaaggctctc ttccgcactc agatgatgcc
      2950       2960       2970       2980       2990       3000
ggggattacg aacagctctt ctcatcaact cagggagaga tctcagagcc gtcccaaata
      3010       3020       3030       3040       3050       3060
acaagtgagc ccgaggaaca aagtggcgct gtcagggaaa gggggctgga ggtttctcat
      3070       3080       3090       3100       3110       3120
cgcctggctc cctggcaggc atctccaccg cacccgtgcc gcttcctatt ggggcctccc
      3130       3140       3150       3160       3170       3180
cagggcggga gtccccgcgg gtctcatcac acaagtgggt cgtccctgtc aacaccccgg
      3190       3200       3210       3220       3230       3240
tcccgtggcg gaacttccca ggtgggctcc ccaaccttgc tgtctccagc tgtgccatca
      3250       3260       3270       3280       3290       3300
aagcagaaaa gggacaggag catcctcacg ctgtctaaag agccagggca ccagaaaggc
      3310       3320       3330       3340       3350       3360
aaagagcgtc ggtccgtgct ggagtgcaga aataaggggg tcctgatgtt cccagaaaaa
      3370       3380       3390       3400       3410       3420
tctccgtcta ttgacctaac ccagtcaaat cctgaccatt cgagctccag atctcagaaa
      3430       3440       3450       3460       3470       3480
tcttcatcca aactgaacga agaagatgag gtcatcctct tactggactc ggatgaggag
      3490       3500       3510       3520       3530       3540
ctggagctag aacaaaccaa aatgaagtcc atttctagtg atcctctgga agaaaagaaa
      3550       3560       3570       3580       3590       3600
gctctagaaa ttagccctag gtcctgtgag ctgttttcca tcattgatgt tgatgcagat
      3610       3620       3630       3640       3650       3660
caggaacctt cccagagccc accaagaagc gaagctgtgc tgcagcagga ggatgagggg
      3670       3680       3690       3700       3710       3720
gcgctgccgg agaatcgggg ctctttgggc aggagagggg ctccctggct gttctgtgac
      3730       3740       3750       3760       3770       3780
cgtgagagca gccccagcga ggccagcacc acagacacct cgtggctggt gcccgccacc
      3790       3800       3810       3820       3830       3840
ccgctggcca gcagaagccg tgactgttct tcccagaccc aaatcagcag cctcaggagc
      3850       3860       3870       3880       3890       3900
gggctggccg tgcaggcggt gactcagcac acgcccaggg cctcagtagg aaacagggaa
      3910       3920       3930       3940       3950       3960
gggaacgaag tcgcacagaa gttttctgtc atcaggcccc agacaccacc gccccagaca
      3970       3980       3990       4000       4010       4020
ccgtcctcat gcctcactcc cgtctctcca ggaacttctg acggcagaag gcaaggccac
      4030       4040       4050       4060       4070       4080
agaagccctt cccgtcccca ccccgggggc cacccgcact cctctccgct ggctccacat
      4090       4100       4110       4120       4130       4140
cccatctcag gggaccgcgc ccacttcagc aggcggttcc tgaaacactc gccgcctggg
      4150       4160       4170       4180       4190       4200
ccaagcttcc tgaaccagac cccagcgggt gaagtggtgg aagtcggaga cagtgacgat
      4210       4220       4230       4240       4250       4260
gagcaggagg tggcctccca tcaggccaac agaagccccc cactggacag tgacccccca
      4270       4280       4290       4300       4310       4320
attccaattg acgactgctg ctggcacatg gagcccctct cgccaattcc cattgaccac
      4330       4340       4350       4360       4370       4380
tggaacctgg agcggaccgg ccccctgagc accagcagcc ccagccgcag gatgaacgag
      4390       4400       4410       4420       4430       4440
gccgccgaca gccgtgactg tcgctccccg ggactcctgg acaccacccc catccgagga
      4450       4460       4470       4480       4490       4500
agctgcacta cccagaggaa attgcaagag aagtcctcgg gcgcgggctc cctggggaat
      4510       4520       4530       4540       4550       4560
agcaggccga gctttctgaa ttcggctctg tgggacgttt gggacgggga agagcagagg
      4570       4580       4590       4600       4610       4620
cctccagaga cccctcctcc ggcccagatg ccaagcgctg gtggagctca gaagcccgaa
      4630       4640       4650       4660       4670       4680
gggttagaga cacccaaagg tgctaatcgg aagaagaact tgccccccaa agtgcccata
      4690       4700       4710       4720       4730       4740
acgccgatgc cacagtattc cattatggag acgccggtgc tgaagaagga actggatagg
      4750       4760       4770       4780       4790       4800
tttggagtcc gccctctgcc taaacgccag atggttctga agctgaagga gatattccag
      4810       4820       4830       4840       4850       4860
tacactcacc agaccctgga ctcagactcc gaggacgaga gccagtcctc acagccgctg
      4870       4880       4890       4900       4910       4920
ttgcaggcgc ctcactgcca gaccctcgcc tcccagacct acaagccttc aagggcaggg
      4930       4940       4950       4960       4970       4980
gtccatgccc agcaggaggc caccacagga cctggggccc ataggcccaa gggacctgct
      4990       5000       5010       5020       5030       5040
aagaccaagg gcccccgaca tcaaaggaag catcatgaaa gcatcacacc cccaagcagg
      5050       5060       5070       5080       5090       5100
tcgcccacca aggaggcacc tccaggcctc aatgatgacg cccagatccc agcctctcaa
      5110       5120       5130       5140       5150       5160
gaatccgtgg ccacctctgt ggatggcagt gacagctcct tgagctcaca gagttcttcc
      5170       5180       5190       5200       5210       5220
tcctgtgagt ttggagcggc atttgagtct gcaggtgaag aggagggcga gggggaggtc
      5230       5240       5250       5260       5270       5280
agtgcctcgc aggcagccgt gcaggcggcg gacacagacg aggcgctgag gtgctacatc
      5290       5300       5310       5320       5330       5340
cgctccaagc cggccctgta ccagaaggtg ctgctgtacc agccctttga gctgcgggag
      5350       5360       5370       5380       5390       5400
ctgcaggcag agctgaggca gaacggcctc cgtgtgtcct cgcgcaggct gttggacttc
      5410       5420       5430       5440       5450       5460
ctggacaccc actgtatcac cttcaccact gccgccaccc gcagggagaa gctccagggc
      5470       5480       5490       5500
aggaggcggc agcctcgggg caagaagaag gtggagcgga actga 

Last modification date: Oct. 2, 2011