Schizo-saccharomyces pombe Lindner (fission yeast) [SPO]

FULL NAME: DNA-(apurinic or apyrimidinic site) lyase 2


DESCRIPTION:
DNA repair enzyme that cleaves apurinic/apyrimidinic (AP) sites and removes 3'-blocking groups present at single strand breaks of damaged DNA. Provides the majority of the AP-endonuclease (APE) activity. Repairs phleomycin D1-induced DNA damage. Plays a role in oxidative damage repair.

STRUCTURE SIMILARITY:
Belongs to the DNA repair enzymes AP/ExoA family.


CATALYTIC ACTIVITY:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.


RELATED PATHWAY(S):
base excision repair (BER)


Amino acids sequence

        10         20         30         40         50         60
MRILSWNVNG IQNPFNYFPW NKKNSYKEIF QELQADVICV QELKMQKDSF PQQYAVVEGF
        70         80         90        100        110        120
DSYFTFPKIR KGYSGVGFYV KKDVAIPVKA EEGITGILPV RGQKYSYSEA PEHEKIGFFP
       130        140        150        160        170        180
KDIDRKTANW IDSEGRCILL DFQMFILIGV YCPVNSGENR LEYRRAFYKA LRERIERLIK
       190        200        210        220        230        240
EGNRKIILVG DVNILCNPID TADQKDIIRE SLIPSIMESR QWIRDLLLPS RLGLLLDIGR
       250        260        270        280        290        300
IQHPTRKGMF TCWNTRLNTR PTNYGTRIDY TLATPDLLPW VQDADIMAEV MGSDHCPVYL
       310        320        330        340        350        360
DLKEEYEGKK LSNFLSHSKE PPLLSTAHHS AYRPSKNIHS MFQHFNSMKK NKNNSPTQSE
       370        380        390        400        410        420
NVSASASSGS SPTVSRANSV IDVDAYPPEK RRRKEQSKLL SFFAKQKEEK EETNKTEDVS
       430        440        450        460        470        480
IEVLDNNNES DIGLTVKKKV ENGNAWKQIF SERAPPLCEG HKEPCKYLTV RKPGINYGRK
       490        500        510        520
FWICARPVGE LIKNSNAVSE EDTQPFQCRF FIWDSDWRAN SKD 

Encoded by apn2 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgcgaattc taagttggaa tgtgaacggt atacagaatc cattcaatta ttttccatgg
        70         80         90        100        110        120
aataaaaaaa attcctacaa agagatattt caagagttac aggctgatgt aatatgtgtt
       130        140        150        160        170        180
caagaattga aaatgcagaa agacagcttt cctcaacaat atgcagttgt tgagggattc
       190        200        210        220        230        240
gacagttatt ttacatttcc taagatcaga aaaggctata gtggggttgg attttatgta
       250        260        270        280        290        300
aaaaaagatg ttgctattcc agtgaaagca gaagagggaa ttactggcat cctgccagta
       310        320        330        340        350        360
cgaggacaaa aatattcata ttcagaagca ccggagcatg aaaagatagg atttttccca
       370        380        390        400        410        420
aaagacattg acagaaaaac tgctaattgg attgatagtg aaggaaggtg tatattatta
       430        440        450        460        470        480
gattttcaga tgttcatcct aatcggtgtt tactgtccag taaactctgg agaaaataga
       490        500        510        520        530        540
ctagaatatc gacgggcctt ttataaagcc ttacgagaaa gaattgaaag attgataaag
       550        560        570        580        590        600
gaaggaaatc gaaaaatcat tctcgttggg gatgtgaata ttttgtgtaa tcctattgac
       610        620        630        640        650        660
actgctgatc aaaaagacat tattcgagag agtcttattc caagcatcat ggaatcaaga
       670        680        690        700        710        720
caatggatac gtgatttatt actaccaagc aggttgggac tactgttgga cattggccgt
       730        740        750        760        770        780
attcaacatc ctactcggaa aggaatgttt acatgttgga atacaagact taatacaagg
       790        800        810        820        830        840
cccacaaatt atggtacgag aattgattac actttggcaa cacccgattt attaccttgg
       850        860        870        880        890        900
gttcaagatg cagatattat ggctgaggtc atgggttcag atcattgccc cgtatattta
       910        920        930        940        950        960
gatttaaaag aagaatatga aggaaaaaag ttgtccaact ttctaagtca ctcaaaagaa
       970        980        990       1000       1010       1020
ccacctttac tatcaacggc gcatcattca gcctatcggc catcaaaaaa tattcattca
      1030       1040       1050       1060       1070       1080
atgttccaac atttcaattc gatgaaaaag aataaaaaca actcacccac tcagagcgaa
      1090       1100       1110       1120       1130       1140
aacgtatctg cttctgcgtc cagcggctcc tcaccaactg tttctcgtgc aaactctgtc
      1150       1160       1170       1180       1190       1200
atagacgtcg atgcctaccc accggaaaag agaagacgaa aagaacaatc gaagcttctt
      1210       1220       1230       1240       1250       1260
tcattttttg cgaagcaaaa ggaagagaaa gaagaaacca ataaaacaga agacgtttcg
      1270       1280       1290       1300       1310       1320
attgaggtcc ttgataacaa taatgaatct gacatagggt tgacagtaaa aaaaaaagta
      1330       1340       1350       1360       1370       1380
gaaaacggga acgcctggaa gcaaatattc agtgaacgag ctcctccact ttgtgagggt
      1390       1400       1410       1420       1430       1440
cacaaggaac cttgcaagta tttgacagtg aggaagccgg gaatcaatta cgggcggaag
      1450       1460       1470       1480       1490       1500
ttttggattt gtgccagacc ggttggtgaa ctgattaaaa actcaaatgc agtttctgag
      1510       1520       1530       1540       1550       1560
gaagatactc aaccgttcca atgcaggttc tttatctggg actcagattg gagagcaaat
      1570
tcaaaggact ga    

Last modification date: Oct. 2, 2011