Mus musculus L. (mouse) [MMU]

FULL NAME: 5' exonuclease Apollo


DESCRIPTION:
5'-3' exonuclease that plays a central role in telomere maintenance and protection during S-phase. Participates in the protection of telomeres against non-homologous end-joining (NHEJ)-mediated repair, thereby ensuring that telomeres do not fuse. Plays a key role in telomeric loop (T loop) formation by being recruited by TERF2 at the leading end telomeres and by processing leading-end telomeres immediately after their replication via its exonuclease activity: generates 3' single-stranded overhang at the leading end telomeres avoiding blunt leading-end telomeres that are vulnerable to end-joining reactions and expose the telomere end in a manner that activates the DNA repair pathways. Together with TERF2, required to protect telomeres from replicative damage during replication by controlling the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Also involved in response to DNA damage: plays a role in response to DNA interstrand cross-links (ICLs) by facilitating double-strand break formation. In case of spindle stress, involved in prophase checkpoint.

STRUCTURE SIMILARITY:
Belongs to the DNA repair metallo-beta-lactamase (DRMBL) family.


POST-TRANSLATIONAL MODIFICATION:
Ubiquitinated, leading to its degradation. Interaction with TERF2 protects it from ubiquitination (By similarity).


RELATED PATHWAY(S):
Fanconi anemia (FA) pathway


Amino acids sequence

        10         20         30         40         50         60
MLKEPALILG KQIHTLYLDN TNCNPALVLP SRQEATQQIV QLIRQFPQHN IKIGLYSLGK
        70         80         90        100        110        120
ESLLEQLALE FRTWVVLSPQ RLELVQLLGL ADVFTVEEEA GRIHAVDHTE ICHSAMLQWN
       130        140        150        160        170        180
QSHPTIAIFP TSRKVRSPHP SIYTVPYSDH SSYSELRAFV AALRPCQVVP IVHQKPCGEF
       190        200        210        220        230        240
FQDSLSPRLA MPLIPHSVQQ YMSSSSRKTN VLWQLERRLK RPRTQGVVFE SPEEKANQVK
       250        260        270        280        290        300
VDRDSKKHKK ENLSPWAGHL ERLCPHPLQA RKQLFPDFCR KERDEPVLFC DSNKMATVLT
       310        320        330        340        350        360
APLEFSVQLQ PIDEFLFPET REKIGLESPL LSRGDSGSPA RGNQSDCVGC GSPPAHISRA
       370        380        390        400        410
VPLTPESRGL ALKYLLTPVH FLQAGFSSRN FDKQVEKHQR VQRSSPAVLS PVDVG

Encoded by Dclre1b gene

FULL NAME: DNA cross-link repair 1B, PSO2 homolog (S. cerevisiae)


OTHER NAME(S):
AI452214
Apollo
SNMIB
mSNM1B


Nucleic acid sequence

        10         20         30         40         50         60
atgctgaagg aacctgctct gatactgggg aaacagattc atactttgta tctagacaac
        70         80         90        100        110        120
actaattgca atccagccct ggttcttcct tcccgacaag aggctactca gcagattgtc
       130        140        150        160        170        180
cagctaatcc gacagttccc acaacacaac ataaaaattg gactctatag tctaggaaaa
       190        200        210        220        230        240
gaatcactgc tggagcaact agcccttgag tttcggacct gggtggtatt aagtcctcaa
       250        260        270        280        290        300
cgcctggagt tggtacagtt gctgggcctg gcagacgtgt tcacagttga ggaagaagcc
       310        320        330        340        350        360
gggcgcatcc atgctgtgga ccatacggag atctgccact ctgccatgct tcagtggaac
       370        380        390        400        410        420
cagagtcacc ctaccattgc tattttcccc acaagccgga aagtgcgcag ccctcacccc
       430        440        450        460        470        480
agcatctaca ccgtcccgta ctctgaccat tcatcttact ccgagctccg tgcttttgtg
       490        500        510        520        530        540
gcagcgctga ggccttgtca ggtggtgccc atcgtccatc aaaagccttg tggagagttc
       550        560        570        580        590        600
tttcaggaca gcttgagtcc taggctcgct atgcctctga ttccacactc tgtgcagcag
       610        620        630        640        650        660
tacatgagtt cctcctctag gaaaacaaat gtgctttggc agttagaaag gaggctcaag
       670        680        690        700        710        720
aggccaagaa ctcagggtgt tgtgtttgaa tcccctgagg agaaagctaa tcaggttaaa
       730        740        750        760        770        780
gttgacagag actcaaagaa gcacaaaaag gaaaacctct ctccctgggc tgggcacctt
       790        800        810        820        830        840
gagaggcttt gcccccatcc tctgcaggcc aggaagcagt tgttcccaga cttctgcagg
       850        860        870        880        890        900
aaagaacgtg atgagccagt cctcttttgt gactccaaca agatggcgac tgtgttgact
       910        920        930        940        950        960
gccccactgg aattctcagt gcaattacaa cctatagatg agtttctctt tccagaaacc
       970        980        990       1000       1010       1020
agggagaaaa ttggcttaga gtccccattg ctgtcaaggg gagacagtgg ctcaccagcg
      1030       1040       1050       1060       1070       1080
agagggaacc aaagtgactg cgtaggctgt ggttctcccc cggctcacat tagcagagct
      1090       1100       1110       1120       1130       1140
gttcctctaa ctcctgagtc caggggcctg gcgctaaaat accttctcac tccagtgcat
      1150       1160       1170       1180       1190       1200
ttcctccaag caggcttctc ttccaggaac ttcgacaagc aagtggaaaa acaccagaga
      1210       1220       1230       1240
gtacagcgta gcagtcctgc agttctgagc ccggtggatg ttggctag 

Last modification date: Oct. 2, 2011