Schizo-saccharomyces pombe Lindner (fission yeast) [SPO]

FULL NAME: DNA-3-methyladenine glycosylase 1


DESCRIPTION:
Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine or 7-methyladenine from the damaged DNA polymer formed by alkylation lesions. Can release ethylated and propylated bases from DNA in addition to 3-methyladenine.

STRUCTURE SIMILARITY:
Belongs to the alkylbase DNA glycosidase AlkA family.


CATALYTIC ACTIVITY:
Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.


RELATED PATHWAY(S):
base excision repair (BER)


Amino acids sequence

        10         20         30         40         50         60
MTLDIEEKEE IVTSLTKAEI HLSGLDENWK RLVKLVGNYR PNRSMEKKEP YEELIRAVAS
        70         80         90        100        110        120
QQLHSKAANA IFNRFKSISN NGQFPTPEEI RDMDFEIMRA CGFSARKIDS LKSIAEATIS
       130        140        150        160        170        180
GLIPTKEEAE RLSNEELIER LTQIKGIGRW TVEMLLIFSL NRDDVMPADD LSIRNGYRYL
       190        200        210        220
HRLPKIPTKM YVLKHSEICA PFRTAAAWYL WKTSKLADYT KPVRPKKH 

Encoded by mag1 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgactttgg acattgaaga gaaagaagag atagtcactt cactcacaaa agcagaaatc
        70         80         90        100        110        120
catctttctg gattggatga gaattggaaa cggctcgtga agctcgttgg taattatcga
       130        140        150        160        170        180
cccaatcgaa gtatggagaa aaaagagcct tatgaagaat taattcgtgc ggtagcatct
       190        200        210        220        230        240
caacaattgc atagtaaagc tgctaacgca attttcaatc gctttaaaag tatttctaac
       250        260        270        280        290        300
aatggccaat ttccaacacc agaggaaatt cgtgatatgg attttgaaat aatgcgtgcc
       310        320        330        340        350        360
tgtggatttt cagctcgtaa aattgattca ttaaaaagca ttgctgaggc aacaatcagc
       370        380        390        400        410        420
ggtttgattc ctactaaaga agaagctgaa aggctctcaa atgaagaatt gatagaacga
       430        440        450        460        470        480
cttactcaga ttaaaggaat cggaagatgg acagtagaaa tgttactcat cttttcgttg
       490        500        510        520        530        540
aatcgtgatg atgttatgcc agctgacgat ttgagtattc gtaatggata ccgatatcta
       550        560        570        580        590        600
caccgattac caaaaattcc aaccaagatg tatgttctta agcatagtga aatatgtgca
       610        620        630        640        650        660
ccttttcgta ctgcagcagc atggtatctc tggaaaacgt caaaacttgc cgattataca
       670        680
aagcctgtta ggccgaagaa acactga   

Last modification date: Oct. 2, 2011