Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: DNA-(apurinic or apyrimidinic site) lyase 2


DESCRIPTION:
DNA repair enzyme that cleaves apurinic/apyrimidinic (AP) sites and removes 3'-blocking groups present at single strand breaks of damaged DNA.

MISCELLANEOUS:
Present with 414 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Belongs to the DNA repair enzymes AP/ExoA family.


CATALYTIC ACTIVITY:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.


RELATED PATHWAY(S):
base excision repair (BER)


Amino acids sequence

        10         20         30         40         50         60
MSSSENTLLD GKSENTIRFL TFNVNGIRTF FHYQPFSQMN QSLRSVFDFF RADIITFQEL
        70         80         90        100        110        120
KTEKLSISKW GRVDGFYSFI SIPQTRKGYS GVGCWIRIPE KNHPLYHALQ VVKAEEGITG
       130        140        150        160        170        180
YLTIKNGKHS AISYRNDVNQ GIGGYDSLDP DLDEKSALEL DSEGRCVMVE LACGIVIISV
       190        200        210        220        230        240
YCPANSNSSE EGEMFRLRFL KVLLRRVRNL DKIGKKIVLM GDVNVCRDLI DSADTLEQFS
       250        260        270        280        290        300
IPITDPMGGT KLEAQYRDKA IQFIINPDTP HRRIFNQILA DSLLPDASKR GILIDTTRLI
       310        320        330        340        350        360
QTRNRLKMYT VWNMLKNLRP SNYGSRIDFI LVSLKLERCI KAADILPDIL GSDHCPVYSD
       370        380        390        400        410        420
LDILDDRIEP GTTQVPIPKF EARYKYNLRN HNVLEMFAKK DTNKESNKQK YCVSKVMNTK
       430        440        450        460        470        480
KNSNIKNKSL DSFFQKVNGE KDDRIKESSE IPQQAKKRIS TPKLNFKDVF GKPPLCRHGE
       490        500        510        520
ESMLKTSKTS ANPGRKFWIC KRSRGDSNNT ESSCGFFQWV  

Encoded by APN2 gene


OTHER NAME(S):
ETH1


Nucleic acid sequence

        10         20         30         40         50         60
atgtcatcaa gcgaaaacac gttactggat ggaaagtcag agaacacaat acgattttta
        70         80         90        100        110        120
actttcaatg tcaatggtat aagaaccttt tttcattatc aaccattttc tcaaatgaat
       130        140        150        160        170        180
caatccctta gatctgtttt cgactttttt cgagcagaca taataacatt ccaagagctc
       190        200        210        220        230        240
aagacggaaa aattgtctat ctctaagtgg gggagagttg atggttttta ttcttttatt
       250        260        270        280        290        300
tctatccctc aaaccagaaa gggatattct ggcgttggct gctggattag aattccggaa
       310        320        330        340        350        360
aagaaccacc cactatacca tgcattacaa gtcgttaagg cagaagaagg tataacgggt
       370        380        390        400        410        420
tacttgacaa taaaaaatgg taagcattca gcaatctcct atagaaacga cgtaaatcaa
       430        440        450        460        470        480
ggaattggtg gttacgattc tttagatccc gatttagatg agaaaagtgc actggaacta
       490        500        510        520        530        540
gattcagaag gcagatgtgt tatggttgaa ctggcatgtg gaatagttat tatcagtgta
       550        560        570        580        590        600
tattgtcccg caaattcgaa ctcatcggag gagggtgaga tgtttagatt aaggttcttg
       610        620        630        640        650        660
aaagttttat taagaagagt tcggaatttg gacaaaattg ggaagaagat tgtgctaatg
       670        680        690        700        710        720
ggcgacgtaa atgtttgccg ggatcttata gacagtgccg atacattaga acaattctca
       730        740        750        760        770        780
attccaataa cagatcccat gggtggaaca aagttagaag cacaatatag ggataaagca
       790        800        810        820        830        840
atccaattta ttatcaatcc ggacacgcca catcggagga tatttaatca aatattggct
       850        860        870        880        890        900
gattcacttt taccagacgc gagtaaaagg gggatactga tagacactac gaggctaatt
       910        920        930        940        950        960
caaacaagaa atcgacttaa aatgtataca gtctggaata tgttaaaaaa tttaagacct
       970        980        990       1000       1010       1020
tcgaattatg gctcacggat agattttatc ctagtgtcct taaagcttga acgatgcata
      1030       1040       1050       1060       1070       1080
aaagcagctg acattcttcc ggatatattg ggctctgacc attgtcctgt gtattctgat
      1090       1100       1110       1120       1130       1140
ttagatatac tggacgacag aattgaacct ggtacgacac aagttcccat accaaaattc
      1150       1160       1170       1180       1190       1200
gaagcaaggt acaaatataa tttaagaaac cataatgttt tagagatgtt tgccaaaaag
      1210       1220       1230       1240       1250       1260
gatacgaata aagaatctaa taaacaaaaa tattgtgtat caaaagtcat gaataccaaa
      1270       1280       1290       1300       1310       1320
aaaaacagca acatcaaaaa caaatcgctc gactcatttt tccagaaggt aaatggagaa
      1330       1340       1350       1360       1370       1380
aaagatgaca ggattaaaga atcctctgaa attccacagc aagctaaaaa aagaatctcc
      1390       1400       1410       1420       1430       1440
acgccaaagt tgaatttcaa ggatgtcttt ggaaagcctc ccctgtgcag gcatggggag
      1450       1460       1470       1480       1490       1500
gaatccatgc tgaaaacatc gaaaacttcg gccaatccag gtagaaagtt ctggatttgc
      1510       1520       1530       1540       1550       1560
aagagatctc ggggtgattc aaataataca gaatcatctt gtgggttttt tcagtgggtt

taa     

Last modification date: Oct. 2, 2011