Homo sapiens L. (human) [HSA]

FULL NAME: Proliferating cell nuclear antigen


DESCRIPTION:
This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2.

MISCELLANEOUS:
Antibodies against PCNA are present in sera from patients with systemic lupus erythematosus.


STRUCTURE SIMILARITY:
Belongs to the PCNA family.


POST-TRANSLATIONAL MODIFICATION:
Upon methyl methanesulfonate-induced DNA damage, mono-ubiquitinated by the UBE2B-RAD18 complex on Lys-164. This induces non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH, which is required for DNA repair. 'Lys-63' polyubiquitination prevents genomic instability on DNA damage. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis.
Acetylated in response to UV irradiation. Acetylation disrupts interaction with NUDT15 and promotes degradation.


PROTEIN TYPE(S):
accessory protein


RELATED PATHWAY(S):
DNA replication
base excision repair (BER)
nucleotide excision repair (NER)
mismatch repair (MMR)


Amino acids sequence

        10         20         30         40         50         60
MFEARLVQGS ILKKVLEALK DLINEACWDI SSSGVNLQSM DSSHVSLVQL TLRSEGFDTY
        70         80         90        100        110        120
RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVFEAPNQEK VSDYEMKLMD
       130        140        150        160        170        180
LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI
       190        200        210        220        230        240
KLSQTSNVDK EEEAVTIEMN EPVQLTFALR YLNFFTKATP LSSTVTLSMS ADVPLVVEYK
       250        260
IADMGHLKYY LAPKIEDEEG S   

Encoded by PCNA gene

FULL NAME: proliferating cell nuclear antigen


OTHER NAME(S):
MGC8367


DESCRIPTION:
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]


Nucleic acid sequence

        10         20         30         40         50         60
atgttcgagg cgcgcctggt ccagggctcc atcctcaaga aggtgttgga ggcactcaag
        70         80         90        100        110        120
gacctcatca acgaggcctg ctgggatatt agctccagcg gtgtaaacct gcagagcatg
       130        140        150        160        170        180
gactcgtccc acgtctcttt ggtgcagctc accctgcggt ctgagggctt cgacacctac
       190        200        210        220        230        240
cgctgcgacc gcaacctggc catgggcgtg aacctcacca gtatgtccaa aatactaaaa
       250        260        270        280        290        300
tgcgccggca atgaagatat cattacacta agggccgaag ataacgcgga taccttggcg
       310        320        330        340        350        360
ctagtatttg aagcaccaaa ccaggagaaa gtttcagact atgaaatgaa gttgatggat
       370        380        390        400        410        420
ttagatgttg aacaacttgg aattccagaa caggagtaca gctgtgtagt aaagatgcct
       430        440        450        460        470        480
tctggtgaat ttgcacgtat atgccgagat ctcagccata ttggagatgc tgttgtaatt
       490        500        510        520        530        540
tcctgtgcaa aagacggagt gaaattttct gcaagtggag aacttggaaa tggaaacatt
       550        560        570        580        590        600
aaattgtcac agacaagtaa tgtcgataaa gaggaggaag ctgttaccat agagatgaat
       610        620        630        640        650        660
gaaccagttc aactaacttt tgcactgagg tacctgaact tctttacaaa agccactcca
       670        680        690        700        710        720
ctctcttcaa cggtgacact cagtatgtct gcagatgtac cccttgttgt agagtataaa
       730        740        750        760        770        780
attgcggata tgggacactt aaaatactac ttggctccca agatcgagga tgaagaagga

tcttag     

Last modification date: Oct. 2, 2011