Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: DNA-3-methyladenine glycosylase


DESCRIPTION:
Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine or 7-methyladenine from the damaged DNA polymer formed by alkylation lesions.

STRUCTURE SIMILARITY:
Belongs to the alkylbase DNA glycosidase AlkA family.


CATALYTIC ACTIVITY:
Hydrolysis of alkylated DNA, releasing 3-methyladenine, 3-methylguanine, 7-methylguanine and 7-methyladenine.


RELATED PATHWAY(S):
base excision repair (BER)


Amino acids sequence

        10         20         30         40         50         60
MKLKREYDEL IKADAVKEIA KELGSRPLEV ALPEKYIARH EEKFNMACEH ILEKDPSLFP
        70         80         90        100        110        120
ILKNNEFTLY LKETQVPNTL EDYFIRLAST ILSQQISGQA AESIKARVVS LYGGAFPDYK
       130        140        150        160        170        180
ILFEDFKDPA KCAEIAKCGL SKRKMIYLES LAVYFTEKYK DIEKLFGQKD NDEEVIESLV
       190        200        210        220        230        240
TNVKGIGPWS AKMFLISGLK RMDVFAPEDL GIARGFSKYL SDKPELEKEL MRERKVVKKS
       250        260        270        280        290
KIKHKKYNWK IYDDDIMEKC SETFSPYRSV FMFILWRLAS TNTDAMMKAE ENFVKS

Encoded by MAG1 gene


OTHER NAME(S):
MMS5


Nucleic acid sequence

        10         20         30         40         50         60
atgaaactaa aaagggagta tgatgagtta ataaaagcag acgctgttaa ggaaatagca
        70         80         90        100        110        120
aaagaattag ggtctcgacc tctagaggtt gctcttcctg agaaatatat tgctagacat
       130        140        150        160        170        180
gaagaaaagt tcaatatggc ttgcgaacac attttagaga aagatccatc actttttccc
       190        200        210        220        230        240
atacttaaga ataatgaatt tacgttgtac ttgaaggaga ctcaagtccc taatacactc
       250        260        270        280        290        300
gaagattatt ttattaggct tgcaagcaca attttgtctc aacagatcag tggccaagca
       310        320        330        340        350        360
gctgaaagca tcaaggcaag ggttgtcagt ctttatggcg gtgcatttcc tgattacaaa
       370        380        390        400        410        420
atccttttcg aagacttcaa agacccagca aaatgtgcag aaatcgcaaa atgtggattg
       430        440        450        460        470        480
agtaaaagga aaatgatata tctagagtct cttgctgtct attttactga aaaatataag
       490        500        510        520        530        540
gatatcgaaa agctcttcgg tcaaaaagat aatgatgagg aagtgattga aagtttagtt
       550        560        570        580        590        600
acgaatgtaa aaggtatagg cccatggagt gccaaaatgt tcttgatctc cggattgaaa
       610        620        630        640        650        660
agaatggatg tatttgctcc tgaagatcta ggtattgcta ggggtttttc aaaatacctt
       670        680        690        700        710        720
tcagataagc cagaattgga aaaagaatta atgcgtgaaa gaaaagtagt taaaaagagt
       730        740        750        760        770        780
aagattaagc ataagaaata caactggaaa atatatgacg acgacataat ggaaaaatgc
       790        800        810        820        830        840
tctgaaacat tttctccgta taggtctgtg tttatgttca tactttggag gctcgcgagc
       850        860        870        880        890
acaaatacag atgccatgat gaaggcagaa gaaaatttcg tgaaatccta a

Last modification date: Oct. 2, 2011