Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: N-glycosylase/DNA lyase


DESCRIPTION:
DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion.

MISCELLANEOUS:
Present with 3690 molecules/cell in log phase SD medium.


STRUCTURE SIMILARITY:
Belongs to the type-1 OGG1 family.


CATALYTIC ACTIVITY:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.


RELATED PATHWAY(S):
base excision repair (BER)


Amino acids sequence

        10         20         30         40         50         60
MSYKFGKLAI NKSELCLANV LQAGQSFRWI WDEKLNQYST TMKIGQQEKY SVVILRQDEE
        70         80         90        100        110        120
NEILEFVAVG DCGNQDALKT HLMKYFRLDV SLKHLFDNVW IPSDKAFAKL SPQGIRILAQ
       130        140        150        160        170        180
EPWETLISFI CSSNNNISRI TRMCNSLCSN FGNLITTIDG VAYHSFPTSE ELTSRATEAK
       190        200        210        220        230        240
LRELGFGYRA KYIIETARKL VNDKAEANIT SDTTYLQSIC KDAQYEDVRE HLMSYNGVGP
       250        260        270        280        290        300
KVADCVCLMG LHMDGIVPVD VHVSRIAKRD YQISANKNHL KELRTKYNAL PISRKKINLE
       310        320        330        340        350        360
LDHIRLMLFK KWGSYAGWAQ GVLFSKEIGG TSGSTTTGTI KKRKWDMIKE TEAIVTKQMK
       370
LKVELSDLHI KEAKID    

Encoded by OGG1 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgtcttata aattcggcaa acttgccatt aataaaagtg agctatgtct agcaaatgtg
        70         80         90        100        110        120
ctacaggctg gccaatcctt tcgatggatt tgggatgaaa agttaaacca gtacagcact
       130        140        150        160        170        180
acaatgaaga taggccagca agaaaagtat tcagtagtga ttttgaggca agatgaagag
       190        200        210        220        230        240
aatgagatcc tggaatttgt tgctgtcggc gactgtggca atcaggacgc cttaaaaact
       250        260        270        280        290        300
catctaatga aatattttag attggacgtg tcactgaagc acttattcga taatgtttgg
       310        320        330        340        350        360
attccaagcg ataaagcatt tgcgaaactt tctccacaag gcatccgtat cttggcccag
       370        380        390        400        410        420
gaaccatggg agacattgat ttcctttatt tgctcgagta ataataacat ttcaagaatt
       430        440        450        460        470        480
acgagaatgt gcaatagcct ttgctctaat ttcgggaact taatcacaac aatagatggg
       490        500        510        520        530        540
gtcgcctacc attcgtttcc tacaagtgag gagctaactt ctcgagccac tgaagccaag
       550        560        570        580        590        600
ttgcgtgagc tgggctttgg atatagagct aagtatatta tcgaaaccgc gagaaaactg
       610        620        630        640        650        660
gtgaatgata aagctgaagc taatattact tctgacacca cataccttca aagtatctgt
       670        680        690        700        710        720
aaagatgctc aatatgaaga cgttagagag cacctcatgt cctacaatgg tgtaggcccc
       730        740        750        760        770        780
aaagttgctg attgcgtttg tctaatggga cttcacatgg atggcatcgt ccccgtagat
       790        800        810        820        830        840
gtccatgtta gtagaattgc gaagagggac taccaaatat ctgcgaacaa gaaccatctc
       850        860        870        880        890        900
aaagaattga ggacaaaata caacgcctta cctatctcaa gaaaaaaaat caacctagag
       910        920        930        940        950        960
ctcgaccata ttagattaat gctttttaaa aaatggggat catacgctgg ttgggcccaa
       970        980        990       1000       1010       1020
ggtgtcctat tctctaaaga aatcggaggg actagtggta gcactacaac tggtactata
      1030       1040       1050       1060       1070       1080
aagaagagaa aatgggatat gataaaggaa acggaagcaa ttgttacgaa acaaatgaaa
      1090       1100       1110       1120       1130
ctgaaagttg aattgtctga tcttcacatc aaagaagcaa aaatagatta g

Last modification date: Oct. 2, 2011