Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: Single-stranded-DNA-specific exonuclease recJ


DESCRIPTION:
Single-stranded-DNA-specific exonuclease. Required for many types of recombinational events, although the stringency of the requirement for recJ appears to vary with the type of recombinational event monitored and the other recombination gene products which are available.

STRUCTURE SIMILARITY:
Belongs to the recJ family.


PROTEIN TYPE(S):
5'-3' exonuclease
DNase
dRP-lyase


RELATED PATHWAY(S):
base excision repair (BER)
mismatch repair (MMR)
homologous recombination (HR)


RELATED DAMAGE:
5'-P end
5'-dRP termini


Amino acids sequence

        10         20         30         40         50         60
MKQQIQLRRR EVDETADLPA ELPPLLRRLY ASRGVRSAQE LERSVKGMLP WQQLSGVEKA
        70         80         90        100        110        120
VEILYNAFRE GTRIIVVGDF DADGATSTAL SVLAMRSLGC SNIDYLVPNR FEDGYGLSPE
       130        140        150        160        170        180
VVDQAHARGA QLIVTVDNGI SSHAGVEHAR SLGIPVIVTD HHLPGDTLPA AEAIINPNLR
       190        200        210        220        230        240
DCNFPSKSLA GVGVAFYLML ALRTFLRDQG WFDERNIAIP NLAELLDLVA LGTVADVVPL
       250        260        270        280        290        300
DANNRILTWQ GMSRIRAGKC RPGIKALLEV ANRDAQKLAA SDLGFALGPR LNAAGRLDDM
       310        320        330        340        350        360
SVGVALLLCD NIGEARVLAN ELDALNQTRK EIEQGMQIEA LTLCEKLERS RDTLPGGLAM
       370        380        390        400        410        420
YHPEWHQGVV GILASRIKER FHRPVIAFAP AGDGTLKGSG RSIQGLHMRD ALERLDTLYP
       430        440        450        460        470        480
GMMLKFGGHA MAAGLSLEED KFKLFQQRFG ELVTEWLDPS LLQGEVVSDG PLSPAEMTME
       490        500        510        520        530        540
VAQLLRDAGP WGQMFPEPLF DGHFRLLQQR LVGERHLKVM VEPVGGGPLL DGIAFNVDTA
       550        560        570
LWPDNGVREV QLAYKLDINE FRGNRSLQII IDNIWPI  

Encoded by recJ gene


OTHER NAME(S):
ECK2887
JW2860


DESCRIPTION:
Lovett and Kolodner (1989) N-terminally sequenced RecJ, but the native RBS was not used to direct translation, so it has been removed from the Verified Set. [More information is available at EcoGene: EG10830]. Three essential activities for the initiation of recombination in the recB1080 mutant are provided by different proteins: helicase activity by RecB1080CD, 5' --> 3' exonuclease by RecJ, and RecA-single-stranded DNA filament formation by RecFOR. [More information is available at EcoCyc: EG10830].


Nucleic acid sequence

        10         20         30         40         50         60
gtgaaacaac agatacaact tcgtcgccgt gaagtcgatg aaacggcaga cttgcccgct
        70         80         90        100        110        120
gaattgcctc ccttgctgcg ccgtttatac gccagccggg gagtacgcag tgcgcaagaa
       130        140        150        160        170        180
ctggaacgca gtgttaaagg tatgctgccc tggcagcaac tgagcggcgt cgaaaaggcc
       190        200        210        220        230        240
gttgagatcc tttacaacgc ttttcgcgaa ggaacgcgga ttattgtggt cggtgatttc
       250        260        270        280        290        300
gacgccgacg gcgcgaccag cacggctcta agcgtgctgg cgatgcgctc gcttggttgc
       310        320        330        340        350        360
agcaatatcg actacctggt accaaaccgt ttcgaagacg gttacggctt aagcccggaa
       370        380        390        400        410        420
gtggtcgatc aggcccatgc ccgtggcgcg cagttaattg tcacggtgga taacggtatt
       430        440        450        460        470        480
tcctcccatg cgggggttga gcacgctcgc tcgttgggca tcccggttat tgttaccgat
       490        500        510        520        530        540
caccatttgc caggcgacac attacccgca gcggaagcga tcattaaccc taacttgcgc
       550        560        570        580        590        600
gactgtaatt tcccgtcgaa atcactggca ggcgtgggtg tggcgtttta tctgatgctg
       610        620        630        640        650        660
gcgctgcgca cctttttgcg cgatcagggc tggtttgatg agcgtaacat cgcaattcct
       670        680        690        700        710        720
aacctggcag aactgctgga tctggtcgcg ctggggacag tggcggacgt cgtgccgctg
       730        740        750        760        770        780
gacgctaata atcgcattct gacctggcag gggatgagtc gcatccgagc cggaaagtgc
       790        800        810        820        830        840
cgtccgggga ttaaagcgct gcttgaagtg gcaaaccgtg atgcacaaaa actcgccgcc
       850        860        870        880        890        900
agcgatttag gttttgcgct ggggccacgt ctcaatgctg ccggacgact ggacgatatg
       910        920        930        940        950        960
tccgtcggtg tggcgctgtt gttgtgcgac aacatcggcg aagcgcgcgt gctggcaaat
       970        980        990       1000       1010       1020
gaactcgatg cgctaaacca gacgcgaaaa gagatcgaac aaggaatgca aattgaagcc
      1030       1040       1050       1060       1070       1080
ctgaccctgt gcgagaaact ggagcgcagc cgtgacacgc tacccggcgg gctggcaatg
      1090       1100       1110       1120       1130       1140
tatcaccccg aatggcatca gggcgttgtc ggtattctgg cttcgcgcat caaagagcgt
      1150       1160       1170       1180       1190       1200
tttcaccgtc cggttatcgc gtttgcgcca gcaggtgacg gtacgctgaa aggttccggt
      1210       1220       1230       1240       1250       1260
cgctccattc aggggctgca tatgcgtgat gcgctggagc gattagacac actctaccct
      1270       1280       1290       1300       1310       1320
ggcatgatgc tgaagtttgg cggtcatgcg atggcggcgg gtttgtcgct ggaagaggat
      1330       1340       1350       1360       1370       1380
aaattcaaac tctttcaaca acggtttggc gaactggtta ctgagtggct ggacccttcg
      1390       1400       1410       1420       1430       1440
ctattgcaag gcgaagtggt atcagacggt ccgttaagcc cggccgaaat gaccatggaa
      1450       1460       1470       1480       1490       1500
gtggcgcagc tgctgcgcga tgctggcccg tgggggcaga tgttcccgga gccgctgttt
      1510       1520       1530       1540       1550       1560
gacggtcatt tccgtctgct gcaacagcgg ctggtgggcg aacgtcattt gaaggtgatg
      1570       1580       1590       1600       1610       1620
gtcgaaccgg tcggcggcgg tccactgctg gatggtattg cttttaatgt cgataccgcc
      1630       1640       1650       1660       1670       1680
ctctggccgg ataacggcgt gcgcgaagtg caactggctt ataagctcga tatcaacgag
      1690       1700       1710       1720       1730
tttcgcggca accgcagcct gcaaattatc atcgacaata tctggccaat ttag

Last modification date: Oct. 2, 2011