Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: Endonuclease 4


DESCRIPTION:
Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin.

STRUCTURE SIMILARITY:
Belongs to the AP endonuclease 2 family.


CATALYTIC ACTIVITY:
Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.


PROTEIN TYPE(S):
3'-5' exonuclease
DNase
endonuclease
AP-lyase


RELATED PATHWAY(S):
nucleotide incision repair (NIR)
base excision repair (BER)


RELATED DAMAGE:
3'-OH end
3'-P
3'-PUA termini
AP-site


Amino acids sequence

        10         20         30         40         50         60
MKYIGAHVSA AGGLANAAIR AAEIDATAFA LFTKNQRQWR AAPLTTQTID EFKAACEKYH
        70         80         90        100        110        120
YTSAQILPHD SYLINLGHPV TEALEKSRDA FIDEMQRCEQ LGLSLLNFHP GSHLMQISEE
       130        140        150        160        170        180
DCLARIAESI NIALDKTQGV TAVIENTAGQ GSNLGFKFEH LAAIIDGVED KSRVGVCIDT
       190        200        210        220        230        240
CHAFAAGYDL RTPAECEKTF ADFARTVGFK YLRGMHLNDA KSTFGSRVDR HHSLGEGNIG
       250        260        270        280
HDAFRWIMQD DRFDGIPLIL ETINPDIWAE EIAWLKAQQT EKAVA 

Encoded by nfo gene


OTHER NAME(S):
ECK2152
JW2146


DESCRIPTION:
Damaged bases in DNA occur due to alkylating agents, ionizing radiation or oxidants such as superoxide radicals, hydrogen peroxide and hydroxyl radicals. [More information is available at EcoCyc: EG10651].


Nucleic acid sequence

        10         20         30         40         50         60
atgaaataca ttggagcgca cgttagtgct gctggcggtc tggcaaatgc cgcaattcgc
        70         80         90        100        110        120
gccgccgaaa tcgacgcaac cgcgtttgcc ttgttcacca aaaaccaacg tcagtggcgt
       130        140        150        160        170        180
gccgcaccgc tcacgacgca aaccatcgat gaattcaaag ccgcctgtga aaaatatcac
       190        200        210        220        230        240
tacacatcgg cgcaaattct tccccacgac agttatctga ttaacctcgg acatccggtc
       250        260        270        280        290        300
actgaagctc tggaaaaatc gcgcgatgcc tttatagatg aaatgcagcg ttgcgaacag
       310        320        330        340        350        360
ctggggcttt ctttgctcaa cttccaccct ggcagccatc tgatgcagat ttcagaagag
       370        380        390        400        410        420
gattgccttg cgcgtattgc cgaatccatc aacattgcgc tggataaaac tcaaggtgtg
       430        440        450        460        470        480
acagcggtga tagaaaacac cgccggtcag ggcagtaact tagggtttaa attcgaacat
       490        500        510        520        530        540
ctcgcggcga ttatcgacgg cgtggaagat aaatcccgcg tcggcgtctg cattgatacc
       550        560        570        580        590        600
tgccatgctt tcgctgccgg gtatgatttg cgtactccag ccgaatgcga gaaaacattc
       610        620        630        640        650        660
gcggattttg cccgtactgt cggctttaag tatctgcgcg ggatgcacct taacgatgcg
       670        680        690        700        710        720
aaaagcacct ttggcagccg cgttgaccgc catcatagcc tcggtgaagg caatatcggt
       730        740        750        760        770        780
catgatgcgt tccgctggat catgcaggac gaccgtttcg acggcattcc gctgatcctc
       790        800        810        820        830        840
gaaaccatca acccggatat ctgggcagaa gagatcgcct ggctgaaagc gcaacaaact
       850
gaaaaagcgg tagcctga    

Last modification date: Oct. 2, 2011