Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: Exodeoxyribonuclease III


DESCRIPTION:
Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase and ribonuclease H activities.

STRUCTURE SIMILARITY:
Belongs to the DNA repair enzymes AP/ExoA family.


CATALYTIC ACTIVITY:
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.


PROTEIN TYPE(S):
3'-5' exonuclease
DNase
endonuclease
AP-lyase


RELATED PATHWAY(S):
base excision repair (BER)


RELATED DAMAGE:
3'-OH end
3'-P
3'-PUA termini
AP-site


Amino acids sequence

        10         20         30         40         50         60
MKFVSFNING LRARPHQLEA IVEKHQPDVI GLQETKVHDD MFPLEEVAKL GYNVFYHGQK
        70         80         90        100        110        120
GHYGVALLTK ETPIAVRRGF PGDDEEAQRR IIMAEIPSLL GNVTVINGYF PQGESRDHPI
       130        140        150        160        170        180
KFPAKAQFYQ NLQNYLETEL KRDNPVLIMG DMNISPTDLD IGIGEENRKR WLRTGKCSFL
       190        200        210        220        230        240
PEEREWMDRL MSWGLVDTFR HANPQTADRF SWFDYRSKGF DDNRGLRIDL LLASQPLAEC
       250        260
CVETGIDYEI RSMEKPSDHA PVWATFRR   

Encoded by xthA gene


OTHER NAME(S):
ECK1747
JW1738
xth


DESCRIPTION:
Exonuclease III (XthA) is one of two 5' apurinic/apyrimidinic (AP) endonucleases (along with endonuclease IV) in Escherichia coli which function in the repair of DNA at sites where damaged bases have been removed by the activity of DNA glycosylases or where bases have been spontaneously lost. [More information is available at EcoCyc: EG11073].


Nucleic acid sequence

        10         20         30         40         50         60
atgaaatttg tctcttttaa tatcaacggc ctgcgcgcca gacctcacca gcttgaagcc
        70         80         90        100        110        120
atcgtcgaaa agcaccaacc ggatgtgatt ggcctgcagg agacaaaagt tcatgacgat
       130        140        150        160        170        180
atgtttccgc tcgaagaggt ggcgaagctc ggctacaacg tgttttatca cgggcagaaa
       190        200        210        220        230        240
ggccattatg gcgtggcgct gctgaccaaa gagacgccga ttgccgtgcg tcgcggcttt
       250        260        270        280        290        300
cccggtgacg acgaagaggc gcagcggcgg attattatgg cggaaatccc ctcactgctg
       310        320        330        340        350        360
ggtaatgtca ccgtgatcaa cggttacttc ccgcagggtg aaagccgcga ccatccgata
       370        380        390        400        410        420
aaattcccgg caaaagcgca gttttatcag aatctgcaaa actacctgga aaccgaactc
       430        440        450        460        470        480
aaacgtgata atccggtact gattatgggc gatatgaata tcagccctac agatctggat
       490        500        510        520        530        540
atcggcattg gcgaagaaaa ccgtaagcgc tggctgcgta ccggtaaatg ctctttcctg
       550        560        570        580        590        600
ccggaagagc gcgaatggat ggacaggctg atgagctggg ggttggtcga taccttccgc
       610        620        630        640        650        660
catgcgaatc cgcaaacagc agatcgtttc tcatggtttg attaccgctc aaaaggtttt
       670        680        690        700        710        720
gacgataacc gtggtctgcg catcgacctg ctgctcgcca gccaaccgct ggcagaatgt
       730        740        750        760        770        780
tgcgtagaaa ccggcatcga ctatgaaatc cgcagcatgg aaaaaccgtc cgatcacgcc
       790        800
cccgtctggg cgaccttccg ccgctaa   

Last modification date: Oct. 2, 2011