Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: G/U mismatch-specific DNA glycosylase


DESCRIPTION:
Excises ethenocytosine and uracil, which can arise by alkylation or deamination of cytosine, respectively, from the corresponding mispairs with guanine in ds-DNA. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and the mispaired base. The complementary strand guanine functions in substrate recognition. Required for DNA damage lesion repair in stationary-phase cells.

STRUCTURE SIMILARITY:
Belongs to the TDG/mug DNA glycosylase family.


CATALYTIC ACTIVITY:
Specifically hydrolyzes mismatched double-stranded DNA and polynucleotides, releasing free uracil.


ENZYME REGULATION:
Subject to strong product inhibition. N-glycosyl hydrolysis proceeds 100-fold faster than product release.


PROTEIN TYPE(S):
DNA N-glycosylase


RELATED PATHWAY(S):
base excision repair (BER)


RELATED DAMAGE:
1,N2-etheno-G (1,2εG) in dsDNA
dU in ssDNA
3,N4-etheno-C (εC) in dsDNA
dU in dsDNA
deoxyuridine
dU from U:G
T from T:G
5-hydroxy-U (5-OH-U)
5-hydroxymethyl U (5-OH-me-U)
3,N4-hydroxypropyl-C (HPC)
5-hydroxy C (5-OH-C)
5-formyl dU (5-foU)
3,N4-alpha-HPC
3,N4-alpha-OH-gamma-methyl-propano-C (mHPC)


Amino acids sequence

        10         20         30         40         50         60
MVEDILAPGL RVVFCGINPG LSSAGTGFPF AHPANRFWKV IYQAGFTDRQ LKPQEAQHLL
        70         80         90        100        110        120
DYRCGVTKLV DRPTVQANEV SKQELHAGGR KLIEKIEDYQ PQALAILGKQ AYEQGFSQRG
       130        140        150        160
AQWGKQTLTI GSTQIWVLPN PSGLSRVSLE KLVEAYRELD QALVVRGR 

Encoded by mug gene


OTHER NAME(S):
dug
ECK3058
JW3040
ygjF


DESCRIPTION:
Endonuclease IV stimulates Mug activity in vitro by promoting disassociation from the DNA and enzyme turnover. [More information is available at EcoGene: EG12717]. Mug is a glycosylase that acts in DNA repair . [More information is available at EcoCyc: EG12717].


Nucleic acid sequence

        10         20         30         40         50         60
atggttgagg atattttggc tccagggtta cgggtcgtgt tttgcggtat caaccctggg
        70         80         90        100        110        120
ctttcatccg ccgggactgg ttttcccttt gctcatccgg caaatcgctt ctggaaggtg
       130        140        150        160        170        180
atatatcagg ccgggtttac cgaccgtcag ttgaagccgc aggaggcaca gcatctgctg
       190        200        210        220        230        240
gattatcgtt gtggcgtcac caaactggta gaccgtccaa cggtgcaagc caatgaagtt
       250        260        270        280        290        300
tcaaagcagg agctacacgc aggcgggcgt aagctgattg aaaaaattga agattatcag
       310        320        330        340        350        360
ccgcaggcgt tggcgattct gggcaaacaa gcatatgaac agggattcag ccagcgcggt
       370        380        390        400        410        420
gcacagtggg ggaaacaaac gctcaccatt ggttcgacgc agatttgggt gctgccaaat
       430        440        450        460        470        480
cccagcggtt taagtcgcgt ttcactggag aaactggttg aagcgtatcg cgagctggac
       490        500
caggcgctgg tagtgcgtgg gcgataa   

Last modification date: Oct. 2, 2011