Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: Uracil-DNA glycosylase


DESCRIPTION:
Excises uracil residues from the DNA which can arise as a result of misincorporation of dUMP residues by DNA polymerase or due to deamination of cytosine.

STRUCTURE SIMILARITY:
Belongs to the uracil-DNA glycosylase family.


CATALYTIC ACTIVITY:
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil.


PROTEIN TYPE(S):
DNA N-glycosylase


RELATED PATHWAY(S):
base excision repair (BER)


RELATED DAMAGE:
dU in dsDNA
dU in ssDNA
deoxyuridine
dU from U:G
5-hydroxy-U (5-OH-U)
5,6-dihydroxy U (5,6-diOH-U)


Amino acids sequence

        10         20         30         40         50         60
MANELTWHDV LAEEKQQPYF LNTLQTVASE RQSGVTIYPP QKDVFNAFRF TELGDVKVVI
        70         80         90        100        110        120
LGQDPYHGPG QAHGLAFSVR PGIAIPPSLL NMYKELENTI PGFTRPNHGY LESWARQGVL
       130        140        150        160        170        180
LLNTVLTVRA GQAHSHASLG WETFTDKVIS LINQHREGVV FLLWGSHAQK KGAIIDKQRH
       190        200        210        220
HVLKAPHPSP LSAHRGFFGC NHFVLANQWL EQRGETPIDW MPVLPAESE 

Encoded by ung gene


OTHER NAME(S):
ECK2578
JW2564


DESCRIPTION:
UDG and Ndk form a complex (Goswami, 2006). [More information is available at EcoGene: EG11058]. Uracil can be generated in DNA as a result of misincorporation by DNA polymerase, spontaneous deamination of cytosine, and deamination of cytosine induced by bisulfite or nitrous acid. [More information is available at EcoCyc: EG11058].


Nucleic acid sequence

        10         20         30         40         50         60
atggctaacg aattaacctg gcatgacgtg ctggctgaag agaagcagca accctatttt
        70         80         90        100        110        120
cttaataccc ttcagaccgt cgccagcgag cggcagtccg gcgtcactat ctacccacca
       130        140        150        160        170        180
caaaaagatg tctttaacgc gttccgcttt acagagttgg gtgacgttaa agtggtgatt
       190        200        210        220        230        240
ctcggccagg atccttatca cggaccggga caggcgcatg gtctggcatt ttccgttcgt
       250        260        270        280        290        300
cccggcattg ccattcctcc gtcattattg aatatgtata aagagctgga aaatactatt
       310        320        330        340        350        360
ccgggcttca cccgccctaa tcatggttat cttgaaagct gggcgcgtca gggcgttctg
       370        380        390        400        410        420
ctactcaata ctgtgttgac ggtacgcgca ggtcaggcgc attcccacgc cagcctcggc
       430        440        450        460        470        480
tgggaaacct tcaccgataa agtgatcagc ctgattaacc agcatcgcga aggcgtggtg
       490        500        510        520        530        540
tttttgttgt ggggatcgca tgcgcaaaag aaaggggcga ttatagataa gcaacgccat
       550        560        570        580        590        600
catgtactga aagcaccgca tccgtcgccg ctttcggcgc atcgtggatt ctttggctgc
       610        620        630        640        650        660
aaccattttg tgctggcaaa tcagtggctg gaacaacgtg gcgagacgcc gattgactgg
       670        680        690
atgccagtat taccggcaga gagtgagtaa   

Last modification date: Oct. 2, 2011