Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: DNA-3-methyladenine glycosylase 1


DESCRIPTION:
Hydrolysis of the deoxyribose N-glycosidic bond to excise 3-methyladenine from the damaged DNA polymer formed by alkylation lesions.

CATALYTIC ACTIVITY:
Hydrolysis of alkylated DNA, releasing 3-methyladenine.


ENZYME REGULATION:
Activity is controlled by product inhibition.


PROTEIN TYPE(S):
DNA N-glycosylase


RELATED PATHWAY(S):
base excision repair (BER)


RELATED DAMAGE:
N7-methyl G (7meG)
3-methyl A (3meA)
N3-methyl G (3meG)


Amino acids sequence

        10         20         30         40         50         60
MERCGWVSQD PLYIAYHDNE WGVPETDSKK LFEMICLEGQ QAGLSWITVL KKRENYRACF
        70         80         90        100        110        120
HQFDPVKVAA MQEEDVERLV QDAGIIRHRG KIQAIIGNAR AYLQMEQNGE PFVDFVWSFV
       130        140        150        160        170        180
NHQPQVTQAT TLSEIPTSTS ASDALSKALK KRGFKFVGTT ICYSFMQACG LVNDHVVGCC

CYPGNKP     

Encoded by tag gene


OTHER NAME(S):
ECK3536
JW3518
tagA


DESCRIPTION:
Tag is constitutively expressed. [More information is available at EcoGene: EG10986]. Alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulfonate (MMS) can produce potentially mutagenic lesions in DNA. [More information is available at EcoCyc: EG10986].


Nucleic acid sequence

        10         20         30         40         50         60
atggaacgtt gcggctgggt gagtcaggac ccgctttata ttgcctacca tgataatgag
        70         80         90        100        110        120
tggggcgtgc ctgaaactga cagtaaaaaa ctgttcgaaa tgatctgcct tgaagggcag
       130        140        150        160        170        180
caggctggat tatcgtggat caccgtcctc aaaaaacgcg aaaactatcg cgcctgcttt
       190        200        210        220        230        240
catcagttcg atccggtgaa ggtcgcagca atgcaggaag aggatgtcga aagactggta
       250        260        270        280        290        300
caggacgccg ggattatccg ccatcgaggg aaaattcagg caattattgg taatgcgcgg
       310        320        330        340        350        360
gcgtacctgc aaatggaaca gaacggcgaa ccgtttgtcg actttgtctg gtcgtttgta
       370        380        390        400        410        420
aatcatcagc cacaggtgac acaagccaca acgttgagcg aaattcccac atctacgtcc
       430        440        450        460        470        480
gcctccgacg ccctatctaa ggcactgaaa aaacgtggtt ttaagtttgt cggcaccaca
       490        500        510        520        530        540
atctgttact cctttatgca ggcatgtggg ctggtgaatg atcatgtggt tggctgctgt
       550        560
tgctatccgg gaaataaacc atga   

Last modification date: Oct. 2, 2011