Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: Endonuclease III


DESCRIPTION:
Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions.

STRUCTURE SIMILARITY:
Belongs to the Nth/MutY family.


CATALYTIC ACTIVITY:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.


PROTEIN TYPE(S):
DNA N-glycosylase/AP-lyase
DNase
endonuclease
AP-lyase


RELATED PATHWAY(S):
base excision repair (BER)


RELATED DAMAGE:
thymine glycol (Tg)
open ring forms of ethenoA
5-hydroxy-6-hydro-T (5-OH-6-H-T)
5-hydroxy-U (5-OH-U)
5-hydroxymethyl U (5-OH-me-U)
5-hydroxy C (5-OH-C)
5-formyl dU (5-foU)
5-hydroxy-6-hydro-U (5-OH-6-H-U)
5,6-dihydro U (5,6-diH-U)
uracil glycol (Ug)
5,6-dihydro T (5,6-diH-T)
FapyA
5,6-dihydroxy C (5,6-diOH-C)
5,6-dihydroxy U (5,6-diOH-U)
5-hydroxy-6-hydro-C (5-OH-6-H-C)


Amino acids sequence

        10         20         30         40         50         60
MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA TAKLYPVANT
        70         80         90        100        110        120
PAAMLELGVE GVKTYIKTIG LYNSKAENII KTCRILLEQH NGEVPEDRAA LEALPGVGRK
       130        140        150        160        170        180
TANVVLNTAF GWPTIAVDTH IFRVCNRTQF APGKNVEQVE EKLLKVVPAE FKVDCHHWLI
       190        200        210
LHGRYTCIAR KPRCGSCIIE DLCEYKEKVD I  

Encoded by nth gene


OTHER NAME(S):
ECK1629
JW1625


DESCRIPTION:
Nth is specific for damaged pyrimidine sites, particularly Thy. [More information is available at EcoGene: EG10662]. Endonuclease III or Nth is a DNA glycosylase that recognizes pyrimidine residues damaged by ring saturation, ring fragmentation or ring contraction. [More information is available at EcoCyc: EG10662].


Nucleic acid sequence

        10         20         30         40         50         60
atgaataaag caaaacgcct ggagatcctc actcgcctgc gtgagaacaa tcctcatccc
        70         80         90        100        110        120
accaccgagc ttaatttcag ttcgcctttt gaattgctga ttgccgtact gctttccgct
       130        140        150        160        170        180
caggcgaccg atgtcagtgt taataaggcg acggcgaaac tctacccggt ggcgaatacg
       190        200        210        220        230        240
cctgcagcga tgcttgaact gggcgttgaa ggggtgaaaa cctatatcaa aacgattggg
       250        260        270        280        290        300
ctttataaca gcaaagcaga aaatatcatc aaaacctgcc gtatcttgct ggagcagcat
       310        320        330        340        350        360
aatggcgagg ttccggaaga tcgtgctgcg cttgaagccc tgcccggcgt aggtcgtaaa
       370        380        390        400        410        420
acagccaacg tcgtattaaa cactgcattc ggctggccga ctattgctgt cgacacgcac
       430        440        450        460        470        480
attttccgcg tttgtaatcg tactcaattt gcgccgggga aaaacgtcga acaggtagaa
       490        500        510        520        530        540
gaaaagctac tgaaagtggt tccagcagag tttaaagtcg actgccacca ttggttgatc
       550        560        570        580        590        600
ctgcacgggc gttatacctg cattgcccgc aagccccgct gtggctcttg tattattgaa
       610        620        630
gatctttgtg aatacaaaga gaaagttgac atctga  

Last modification date: Oct. 2, 2011