Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: Endonuclease 8


DESCRIPTION:
Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.

STRUCTURE SIMILARITY:
Contains 1 FPG-type zinc finger.
Belongs to the FPG family.


CATALYTIC ACTIVITY:
Removes damaged bases from DNA, leaving an abasic site.
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.


PROTEIN TYPE(S):
DNA N-glycosylase/AP-lyase
DNase
dRP-lyase
endonuclease
AP-lyase


RELATED PATHWAY(S):
base excision repair (BER)


RELATED DAMAGE:
8-oxoG
5,6-dihydro U (5,6-diH-U)
3'-PUA termini
5-hydroxy-6-hydro-T (5-OH-6-H-T)
5-hydroxy-U (5-OH-U)
5-hydroxymethyl U (5-OH-me-U)
5-hydroxy C (5-OH-C)
5-formyl dU (5-foU)
5-hydroxy-6-hydro-U (5-OH-6-H-U)
thymine glycol (Tg)
uracil glycol (Ug)
5,6-dihydro T (5,6-diH-T)
FapyA
5,6-dihydroxy U (5,6-diOH-U)


Amino acids sequence

        10         20         30         40         50         60
MPEGPEIRRA ADNLEAAIKG KPLTDVWFAF PQLKPYQSQL IGQHVTHVET RGKALLTHFS
        70         80         90        100        110        120
NDLTLYSHNQ LYGVWRVVDT GEEPQTTRVL RVKLQTADKT ILLYSASDIE MLTPEQLTTH
       130        140        150        160        170        180
PFLQRVGPDV LDPNLTPEVV KERLLSPRFR NRQFAGLLLD QAFLAGLGNY LRVEILWQVG
       190        200        210        220        230        240
LTGNHKAKDL NAAQLDALAH ALLEIPRFSY ATRGQVDENK HHGALFRFKV FHRDGEPCER
       250        260
CGSIIEKTTL SSRPFYWCPG CQH   

Encoded by nei gene


OTHER NAME(S):
ECK0703
JW0704


DESCRIPTION:
Damaged bases in DNA occur due to alkylating agents, ionizing radiation or oxidants such as superoxide radicals, hydrogen peroxide and hydroxyl radicals. [More information is available at EcoCyc: G6383].


Nucleic acid sequence

        10         20         30         40         50         60
atgcctgaag gcccggagat ccgccgtgca gcggataacc tggaggcggc gatcaaaggc
        70         80         90        100        110        120
aaaccactaa ctgatgtctg gtttgccttc ccgcagttaa aaccttatca atcacaactt
       130        140        150        160        170        180
atcggtcaac acgttaccca tgtggaaacg cgtggtaagg cgttgttaac tcatttttcc
       190        200        210        220        230        240
aacgacttaa cgctctacag ccataatcag ctttacggcg tctggcgcgt ggttgatacc
       250        260        270        280        290        300
ggcgaagagc cgcagaccac gcgagtattg cgggtaaaac tgcaaacggc tgacaaaacc
       310        320        330        340        350        360
attctgcttt atagcgcctc ggatattgag atgttgaccc cggaacaact gaccacgcat
       370        380        390        400        410        420
ccgtttttac aacgcgttgg tcccgatgtg ctggatccga atctgacgcc ggaggtggtg
       430        440        450        460        470        480
aaagaacgat tattgtcgcc gcgctttcgt aaccgtcagt ttgctggatt actgctcgat
       490        500        510        520        530        540
caggcgtttc tggctgggct tggcaattat ttgcgggtgg agatcctctg gcaggttggg
       550        560        570        580        590        600
ttgactggaa atcataaagc gaaagatctc aatgcggcgc aactggatgc actcgcacac
       610        620        630        640        650        660
gcgttactgg agattcctcg attttcctac gctacgcggg ggcaggtgga tgagaataag
       670        680        690        700        710        720
catcatgggg cgctgtttcg ctttaaggtt tttcatcgag atggcgaacc gtgcgaacgt
       730        740        750        760        770        780
tgtggcagca tcattgagaa aaccacgctg tcatctcgcc cgttttactg gtgccctggc
       790
tgccagcact ag    

Last modification date: Oct. 2, 2011