Escherichia coli Migula (bacterium) K-12 MG1655 [ECO]

FULL NAME: Formamidopyrimidine-DNA glycosylase


DESCRIPTION:
Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.

STRUCTURE SIMILARITY:
Contains 1 FPG-type zinc finger.
Belongs to the FPG family.


CATALYTIC ACTIVITY:
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.
Hydrolysis of DNA containing ring-opened 7-methylguanine residues, releasing 2,6-diamino-4-hydroxy-5-(N-methyl)formamidopyrimidine.


PROTEIN TYPE(S):
DNA N-glycosylase/AP-lyase
DNase
dRP-lyase
endonuclease
AP-lyase


RELATED PATHWAY(S):
base excision repair (BER)


RELATED DAMAGE:
N7-methyl G (7meG)
1,N6-etheno-A (1εA)
8-oxoG
Fapy-7meG
8-oxoA
8-hydroxyA (8-OH-A)
3'-PUA termini
5-hydroxy-U (5-OH-U)
5-hydroxymethyl U (5-OH-me-U)
5-hydroxy C (5-OH-C)
1,N6-etheno-A in (1εA) dsDNA
5-formyl dU (5-foU)
thymine glycol (Tg)
uracil glycol (Ug)
5,6-dihydro T (5,6-diH-T)
FapyA
FapyG
open ring forms of ethenoA
aflatoxin-FapyG
imidazole ring-opened N-2-aminofluorene-C8-guanine


Amino acids sequence

        10         20         30         40         50         60
MPELPEVETS RRGIEPHLVG ATILHAVVRN GRLRWPVSEE IYRLSDQPVL SVQRRAKYLL
        70         80         90        100        110        120
LELPEGWIII HLGMSGSLRI LPEELPPEKH DHVDLVMSNG KVLRYTDPRR FGAWLWTKEL
       130        140        150        160        170        180
EGHNVLTHLG PEPLSDDFNG EYLHQKCAKK KTAIKPWLMD NKLVVGVGNI YASESLFAAG
       190        200        210        220        230        240
IHPDRLASSL SLAECELLAR VIKAVLLRSI EQGGTTLKDF LQSDGKPGYF AQELQVYGRK
       250        260
GEPCRVCGTP IVATKHAQRA TFYCRQCQK   

Encoded by mutM gene


OTHER NAME(S):
ECK3625
fpg
JW3610


DESCRIPTION:
MutM (also known as Fpg) is a DNA glycosylase that works in the base excision repair (BER) pathway to repair certain types of pre-mutagenic lesions. [More information is available at EcoCyc: EG10329].


Nucleic acid sequence

        10         20         30         40         50         60
atgcctgaat tacccgaagt tgaaaccagc cgccgcggca tagaaccgca tctcgttggt
        70         80         90        100        110        120
gcaaccattc ttcatgcagt ggtgcgcaac ggacgcttgc gctggccggt ttcagaagag
       130        140        150        160        170        180
atctaccgtt taagcgacca accagtgctt agcgtgcagc ggcgggctaa atatctgctg
       190        200        210        220        230        240
ctggagctgc ctgagggctg gattatcatt catttaggga tgtctggcag cctgcgcatc
       250        260        270        280        290        300
cttccagaag aacttccccc tgaaaagcat gaccatgtgg atttggtgat gagcaacggc
       310        320        330        340        350        360
aaagtgctgc gctacaccga tccgcgccgc tttggtgcct ggctgtggac caaagagctg
       370        380        390        400        410        420
gaagggcata atgtgctgac ccatcttgga ccggagccgc ttagcgacga tttcaatggt
       430        440        450        460        470        480
gagtatctgc atcagaagtg cgcgaagaaa aaaacggcga ttaaaccgtg gctgatggat
       490        500        510        520        530        540
aacaagctgg tggtaggggt agggaatatc tatgccagcg aatcactgtt tgcggcgggg
       550        560        570        580        590        600
atccatccgg atcggctggc gtcatcactg tcgctggcag agtgtgaatt gttagctcgg
       610        620        630        640        650        660
gtgattaaag cggtgttgct gcgttcgatt gagcagggtg gtacaacgct gaaagatttt
       670        680        690        700        710        720
ctgcaaagtg atggtaaacc gggctatttc gctcaggaat tgcaggttta cgggcgaaaa
       730        740        750        760        770        780
ggtgagccgt gtcgggtgtg cggtacgccg attgtggcga ctaaacatgc gcagcgggca
       790        800        810
acgttttatt gtcggcagtg ccagaagtaa   

Last modification date: Oct. 2, 2011