Homo sapiens L. (human) [HSA]

FULL NAME: DNA polymerase beta


DESCRIPTION:
Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases.

STRUCTURE SIMILARITY:
Belongs to the DNA polymerase type-X family.


CATALYTIC ACTIVITY:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).


POST-TRANSLATIONAL MODIFICATION:
Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity.
Ubiquitinated at Lys-41, Lys-61 and Lys-81: monoubiquitinated by HUWE1/ARF-BP1. Monoubiquitinated protein is then the target of STUB1/CHIP, which catalyzes polyubiquitination from monoubiquitin, leading to degradation by the proteasome. USP47 mediates the deubiquitination of monoubiquitinated protein, preventing polyubiquitination by STUB1/CHIP and its subsequent degradation.


PROTEIN TYPE(S):
X-family DNA polymerase
dRP-lyase


RELATED PATHWAY(S):
base excision repair (BER)
translesion synthesis (TLS)


RELATED DAMAGE:
5'-dRP termini


Amino acids sequence

        10         20         30         40         50         60
MSKRKAPQET LNGGITDMLT ELANFEKNVS QAIHKYNAYR KAASVIAKYP HKIKSGAEAK
        70         80         90        100        110        120
KLPGVGTKIA EKIDEFLATG KLRKLEKIRQ DDTSSSINFL TRVSGIGPSA ARKFVDEGIK
       130        140        150        160        170        180
TLEDLRKNED KLNHHQRIGL KYFGDFEKRI PREEMLQMQD IVLNEVKKVD SEYIATVCGS
       190        200        210        220        230        240
FRRGAESSGD MDVLLTHPSF TSESTKQPKL LHQVVEQLQK VHFITDTLSK GETKFMGVCQ
       250        260        270        280        290        300
LPSKNDEKEY PHRRIDIRLI PKDQYYCGVL YFTGSDIFNK NMRAHALEKG FTINEYTIRP
       310        320        330
LGVTGVAGEP LPVDSEKDIF DYIQWKYREP KDRSE  

Encoded by POLB gene

FULL NAME: polymerase (DNA directed), beta


OTHER NAME(S):
MGC125976


DESCRIPTION:
The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011]


Nucleic acid sequence

        10         20         30         40         50         60
atgagcaaac ggaaggcgcc gcaggagact ctcaacgggg gaatcaccga catgctcaca
        70         80         90        100        110        120
gaactcgcaa actttgagaa gaacgtgagc caagctatcc acaagtacaa tgcttacaga
       130        140        150        160        170        180
aaagcagcat ctgttatagc aaaataccca cacaaaataa agagtggagc tgaagctaag
       190        200        210        220        230        240
aaattgcctg gagtaggaac aaaaattgct gaaaagattg atgagttttt agcaactgga
       250        260        270        280        290        300
aaattacgta aactggaaaa gattcggcag gatgatacga gttcatccat caatttcctg
       310        320        330        340        350        360
actcgagtta gtggcattgg tccatctgct gcaaggaagt ttgtagatga aggaattaaa
       370        380        390        400        410        420
acactagaag atctcagaaa aaatgaagat aaattgaacc atcatcagcg aattgggctg
       430        440        450        460        470        480
aaatattttg gggactttga aaaaagaatt cctcgtgaag agatgttaca aatgcaagat
       490        500        510        520        530        540
attgtactaa atgaagttaa aaaagtggat tctgaataca ttgctacagt ctgtggcagt
       550        560        570        580        590        600
ttcagaagag gtgcagagtc cagtggtgac atggatgttc tcctgaccca tcccagcttc
       610        620        630        640        650        660
acttcagaat caaccaaaca gccaaaactg ttacatcagg ttgtggagca gttacaaaag
       670        680        690        700        710        720
gttcatttta tcacagatac cctgtcaaag ggtgagacaa agttcatggg tgtttgccag
       730        740        750        760        770        780
cttcccagta aaaatgatga aaaagaatat ccacacagaa gaattgatat caggttgata
       790        800        810        820        830        840
cccaaagatc agtattactg tggtgttctc tatttcactg ggagtgatat tttcaataag
       850        860        870        880        890        900
aatatgaggg ctcatgccct agaaaagggt ttcacaatca atgagtacac catccgtccc
       910        920        930        940        950        960
ttgggagtca ctggagttgc aggagaaccc ctgccagtgg atagtgaaaa agacatcttt
       970        980        990       1000
gattacatcc agtggaaata ccgggaaccc aaggaccgga gcgaatga 

Last modification date: Oct. 10, 2011