Homo sapiens L. (human) [HSA]

FULL NAME: Replication protein A 32 kDa subunit


DESCRIPTION:
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

POST-TRANSLATIONAL MODIFICATION:
Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). In response to DNA damage, recruited to DNA-repair nuclear foci, as an hypophosphorylated form. The necessary dephosphorylation step is catalyzed by PP4. Subsequent hyperphosphorylation, catalyzed by ATR, is required for RAD51 recruitment to chromatin and efficient DNA repair. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.


RELATED PATHWAY(S):
DNA replication
nucleotide excision repair (NER)
mismatch repair (MMR)
homologous recombination (HR)
Fanconi anemia (FA) pathway


Amino acids sequence

        10         20         30         40         50         60
MWNSGFESYG SSSYGGAGGY TQSPGGFGSP APSQAEKKSR ARAQHIVPCT ISQLLSATLV
        70         80         90        100        110        120
DEVFRIGNVE ISQVTIVGII RHAEKAPTNI VYKIDDMTAA PMDVRQWVDT DDTSSENTVV
       130        140        150        160        170        180
PPETYVKVAG HLRSFQNKKS LVAFKIMPLE DMNEFTTHIL EVINAHMVLS KANSQPSAGR
       190        200        210        220        230        240
APISNPGMSE AGNFGGNSFM PANGLTVAQN QVLNLIKACP RPEGLNFQDL KNQLKHMSVS
       250        260        270
SIKQAVDFLS NEGHIYSTVD DDHFKSTDAE   

Encoded by RPA2 gene

FULL NAME: replication protein A2, 32kDa


OTHER NAME(S):
REPA2
RPA32


Nucleic acid sequence

        10         20         30         40         50         60
atgtggaaca gtggattcga aagctatggc agctcctcat acgggggagc cggcggctac
        70         80         90        100        110        120
acgcagtccc cggggggctt tggatcgccc gcaccttctc aagccgaaaa gaaatcaaga
       130        140        150        160        170        180
gcccgagccc agcacattgt gccctgtact atatctcagc tgctttctgc cactttggtt
       190        200        210        220        230        240
gatgaagtgt tcagaattgg gaatgttgag atttcacagg tcactattgt ggggatcatc
       250        260        270        280        290        300
agacatgcag agaaggctcc aaccaacatt gtttacaaaa tagatgacat gacagctgca
       310        320        330        340        350        360
cccatggacg ttcgccagtg ggttgacaca gatgacacca gcagtgaaaa cactgtggtt
       370        380        390        400        410        420
cctccagaaa catatgtgaa agtggcaggc cacctgagat cttttcagaa caaaaagagc
       430        440        450        460        470        480
ctggtagcct ttaagatcat gcccctggag gatatgaatg agttcaccac acatattctg
       490        500        510        520        530        540
gaagtgatca atgcacacat ggtactaagc aaagccaaca gccagccctc agcagggaga
       550        560        570        580        590        600
gcacctatca gcaatccagg aatgagtgaa gcagggaact ttggtgggaa tagcttcatg
       610        620        630        640        650        660
ccagcaaatg gcctcactgt ggcccaaaac caggtgttga atttgattaa ggcttgtcca
       670        680        690        700        710        720
agacctgaag ggttgaactt tcaggatctc aagaaccagc tgaaacacat gtctgtatcc
       730        740        750        760        770        780
tcaatcaagc aagctgtgga ttttctgagc aatgaggggc acatctattc tactgtggat
       790        800        810
gatgaccatt ttaaatccac agatgcagaa taa  

Last modification date: Oct. 2, 2011