Homo sapiens L. (human) [HSA]

FULL NAME: Endonuclease 8-like 3


DESCRIPTION:
Reports about DNA glycosylase activity are contradictory. A number of references report finding no DNA glycosylase activity and the protein lacks a proline residue at the N-terminus which functions as an active site residue in other members of the FPG family. However, the mouse ortholog has been shown to have both DNA glycosylase and AP lyase activities and PubMed:19170771 demonstrates AP lyase activity. Prefers single-stranded DNA or partially single-stranded DNA structures such as bubble and fork structures to double-stranded DNA in vitro. Displays a broad recognition spectrum, preferring FapyA and FapyG followed by 5-OHU, 5-PHC and 5-OHMH and then Tg and 8-oxoA. No activity on 8-oxoG detected.

STRUCTURE SIMILARITY:
Contains 1 FPG-type zinc finger.
Belongs to the FPG family.
Contains 1 RanBP2-type zinc finger.


CATALYTIC ACTIVITY:
Removes damaged bases from DNA, leaving an abasic site.
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.


PROTEIN TYPE(S):
DNA N-glycosylase/AP-lyase
DNase
endonuclease
AP-lyase


RELATED PATHWAY(S):
base excision repair (BER)


RELATED DAMAGE:
FapyG


Amino acids sequence

        10         20         30         40         50         60
MVEGPGCTLN GEKIRARVLP GQAVTGVRGS ALRSLQGRAL RLAASTVVVS PQAAALNNDS
        70         80         90        100        110        120
SQNVLSLFNG YVYSGVETLG KELFMYFGPK ALRIHFGMKG FIMINPLEYK YKNGASPVLE
       130        140        150        160        170        180
VQLTKDLICF FDSSVELRNS MESQQRIRMM KELDVCSPEF SFLRAESEVK KQKGRMLGDV
       190        200        210        220        230        240
LMDQNVLPGV GNIIKNEALF DSGLHPAVKV CQLTDEQIHH LMKMIRDFSI LFYRCRKAGL
       250        260        270        280        290        300
ALSKHYKVYK RPNCGQCHCR ITVCRFGDNN RMTYFCPHCQ KENPQHVDIC KLPTRNTIIS
       310        320        330        340        350        360
WTSSRVDHVM DSVARKSEEH WTCVVCTLIN KPSSKACDAC LTSRPIDSVL KSEENSTVFS
       370        380        390        400        410        420
HLMKYPCNTF GKPHTEVKIN RKTAFGTTTL VLTDFSNKSS TLERKTKQNQ ILDEEFQNSP
       430        440        450        460        470        480
PASVCLNDIQ HPSKKTTNDI TQPSSKVNIS PTISSESKLF SPAHKKPKTA QYSSPELKSC
       490        500        510        520        530        540
NPGYSNSELQ INMTDGPRTL NPDSPRCSKH NRLCILRVVR KDGENKGRQF YACPLPREAQ
       550        560        570        580        590        600
CGFFEWADLS FPFCNHGKRS TMKTVLKIGP NNGKNFFVCP LGKEKQCNFF QWAENGPGIK

IIPGC     

Encoded by NEIL3 gene

FULL NAME: nei endonuclease VIII-like 3 (E. coli)


OTHER NAME(S):
FGP2
FLJ10858
FPG2
NEI3
hFPG2
hNEI3


DESCRIPTION:
NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).[supplied by OMIM, Mar 2008]


Nucleic acid sequence

        10         20         30         40         50         60
atggtggaag gaccaggctg tactctgaat ggagagaaga ttcgcgcgcg ggtgctcccg
        70         80         90        100        110        120
ggccaggcgg tgaccggcgt gcggggaagc gctctgcgga gtctgcaggg ccgcgccttg
       130        140        150        160        170        180
cggctcgcag cctccacggt tgtggtctcc ccgcaggctg ctgcactgaa taatgattcc
       190        200        210        220        230        240
agccagaatg tcttgagcct gtttaatgga tatgtttaca gtggcgtgga aactttgggg
       250        260        270        280        290        300
aaggagctct ttatgtactt tggaccaaaa gctttacgga ttcatttcgg aatgaaaggc
       310        320        330        340        350        360
ttcatcatga ttaatccact tgagtataaa tataaaaatg gagcttctcc tgttttggaa
       370        380        390        400        410        420
gtgcagctca ccaaagattt gatttgtttc tttgactcat cagtagaact cagaaactca
       430        440        450        460        470        480
atggaaagcc aacagagaat aagaatgatg aaagaattag atgtatgttc acctgaattt
       490        500        510        520        530        540
agtttcttga gagcagaaag tgaagttaaa aaacagaaag gccggatgct aggtgatgtg
       550        560        570        580        590        600
ctaatggatc agaacgtatt gcctggagta gggaacatca tcaaaaatga agctctcttt
       610        620        630        640        650        660
gacagtggtc tccacccagc tgttaaagtt tgtcaattaa cagatgaaca gatccatcac
       670        680        690        700        710        720
ctcatgaaaa tgatacgtga tttcagcatt ctcttttaca ggtgccgtaa agcaggactt
       730        740        750        760        770        780
gctctctcta aacactataa ggtttacaag cgtcctaatt gtggtcagtg ccactgcaga
       790        800        810        820        830        840
ataactgtgt gccgctttgg ggacaataac agaatgacat atttctgtcc tcactgtcaa
       850        860        870        880        890        900
aaagaaaatc ctcaacatgt tgacatatgc aagctaccga ctagaaatac tataatcagt
       910        920        930        940        950        960
tggacatcta gcagggtgga tcatgttatg gactccgtgg ctcggaagtc ggaagagcac
       970        980        990       1000       1010       1020
tggacctgtg tggtgtgtac tttaatcaat aagccctctt ctaaggcatg tgatgcttgc
      1030       1040       1050       1060       1070       1080
ttgacctcaa ggcctattga ttcagtgctc aagagtgaag aaaattctac tgtctttagc
      1090       1100       1110       1120       1130       1140
cacttaatga agtacccgtg taatactttt ggaaaacctc atacagaagt caagatcaac
      1150       1160       1170       1180       1190       1200
agaaaaactg catttggaac tacaactctt gtcttgactg attttagcaa taaatccagt
      1210       1220       1230       1240       1250       1260
actttggaaa gaaaaacaaa gcaaaaccag atactagatg aggagtttca aaactctcct
      1270       1280       1290       1300       1310       1320
cctgctagtg tttgtttgaa tgatatacag cacccctcca agaagacaac aaacgatata
      1330       1340       1350       1360       1370       1380
actcaaccat ccagcaaagt aaacatatca cctacaatca gttcagaatc taaattattt
      1390       1400       1410       1420       1430       1440
agtccagcac ataaaaaacc gaaaacagcc caatactcat caccagagct taaaagctgc
      1450       1460       1470       1480       1490       1500
aaccctggat attctaacag tgaacttcaa attaatatga cagatggccc tcgtacctta
      1510       1520       1530       1540       1550       1560
aatcctgaca gccctcgctg cagtaaacac aaccgcctct gcattctccg agttgtgagg
      1570       1580       1590       1600       1610       1620
aaggatgggg aaaacaaggg caggcagttt tatgcctgtc ctctacctag agaagcacaa
      1630       1640       1650       1660       1670       1680
tgtggatttt ttgaatgggc agatttgtcc ttcccattct gcaaccatgg caagcgttcc
      1690       1700       1710       1720       1730       1740
accatgaaaa cagtattgaa gattggacct aacaatggaa agaatttttt tgtgtgtcct
      1750       1760       1770       1780       1790       1800
cttgggaagg aaaaacaatg caattttttc cagtgggcag aaaatgggcc aggaataaaa
      1810
attattcctg gatgctaa    

Last modification date: Oct. 2, 2011