Arabidopsis thaliana (L.) Heynh. (mouse-ear cress) [ATH]

FULL NAME: DNA ligase 1


DESCRIPTION:
DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair (By similarity).

STRUCTURE SIMILARITY:
Belongs to the ATP-dependent DNA ligase family.


CATALYTIC ACTIVITY:
ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).


RELATED PATHWAY(S):
DNA replication
base excision repair (BER)
nucleotide excision repair (NER)
mismatch repair (MMR)


Amino acids sequence

        10         20         30         40         50         60
MLAIRSSNYL RCIPSLCTKT QISQFSSVLI SFSRQISHLR LSSCHRAMSS SRPSAFDALM
        70         80         90        100        110        120
SNARAAAKKK TPQTTNLSRS PNKRKIGETQ DANLGKTIVS EGTLPKTEDL LEPVSDSANP
       130        140        150        160        170        180
RSDTSSIAED SKTGAKKAKT LSKTDEMKSK IGLLKKKPND FDPEKMSCWE KGERVPFLFV
       190        200        210        220        230        240
ALAFDLISNE SGRIVITDIL CNMLRTVIAT TPEDLVATVY LSANEIAPAH EGVELGIGES
       250        260        270        280        290        300
TIIKAISEAF GRTEDHVKKQ NTELGDLGLV AKGSRSTQTM MFKPEPLTVV KVFDTFRQIA
       310        320        330        340        350        360
KESGKDSNEK KKNRMKALLV ATTDCEPLYL TRLLQAKLRL GFSGQTVLAA LGQAAVYNEE
       370        380        390        400        410        420
HSKPPPNTKS PLEEAAKIVK QVFTVLPVYD IIVPALLSGG VWNLPKTCNF TLGVPIGPML
       430        440        450        460        470        480
AKPTKGVAEI LNKFQDIVFT CEYKYDGERA QIHFMEDGTF EIYSRNAERN TGKYPDVALA
       490        500        510        520        530        540
LSRLKKPSVK SFILDCEVVA FDREKKKILP FQILSTRARK NVNVNDIKVG VCIFAFDMLY
       550        560        570        580        590        600
LNGQQLIQEN LKIRREKLYE SFEEDPGYFQ FATAVTSNDI DEIQKFLDAS VDVGCEGLII
       610        620        630        640        650        660
KTLDSDATYE PAKRSNNWLK LKKDYMDSIG DSVDLVPIAA FHGRGKRTGV YGAFLLACYD
       670        680        690        700        710        720
VDKEEFQSIC KIGTGFSDAM LDERSSSLRS QVIATPKQYY RVGDSLNPDV WFEPTEVWEV
       730        740        750        760        770        780
KAADLTISPV HRAATGIVDP DKGISLRFPR LLRVREDKKP EEATSSEQIA DLYQAQKHNH
       790
PSNEVKGDDD     

Encoded by LIG1 gene


OTHER NAME(S):
ATLIG1
DNA ligase 1
LIG1
T6D22.23


DESCRIPTION:
Encodes the Arabidopsis DNA ligase 1 that provides the major DNA ligase activity in cells and plays a key role in both DNA replication and excision repair pathways. Indispensable for cell viability. AtLIG1 expresses one major and two minor mRNA transcripts differing only in the length of the 5' untranslated leader sequences preceding a common ORF. Translation from the first in-frame start codon produces an AtLIG1 isoform that is targeted exclusively to the mitochondria. Translation initiation from the second in-frame start codon produces an AtLIG1 isoform targeted only to the nucleus.


Nucleic acid sequence

        10         20         30         40         50         60
atgttagcga ttcgatcgtc gaattacctc cgatgcatac cttctctctg tactaaaacc
        70         80         90        100        110        120
caaatctctc aattctcctc cgtgttaatc tcttttagcc gccaaatttc ccacttgcgt
       130        140        150        160        170        180
cttagctcgt gccatagagc catgtcttct tcccgccctt ctgcatttga tgctctcatg
       190        200        210        220        230        240
tccaatgctc gtgcagccgc caagaagaag actccccaga caactaattt gtctcgctcg
       250        260        270        280        290        300
ccgaacaaaa ggaaaattgg agaaacccaa gatgctaatt tgggcaaaac tattgtttcc
       310        320        330        340        350        360
gaaggtacac ttccaaaaac ggaggatctg ctggagcctg tttctgattc tgcaaatccc
       370        380        390        400        410        420
agatcggata catcgtcaat tgcggaggat tcaaagacgg gagcgaaaaa ggctaagact
       430        440        450        460        470        480
ttgagcaaga cagacgaaat gaagagcaaa attggattgc tgaagaagaa gccgaatgat
       490        500        510        520        530        540
ttcgatcctg aaaaaatgtc ttgctgggag aaaggggaga gagttccgtt tctgtttgtc
       550        560        570        580        590        600
gcattggctt ttgatctgat ctcaaatgaa agtggccgga ttgtgattac tgatattctg
       610        620        630        640        650        660
tgtaacatgt tgagaactgt tattgccact actcctgagg atttggtcgc tactgtttat
       670        680        690        700        710        720
ctttcggcga atgagattgc cccggctcat gaaggtgtag aattgggaat tggtgagagt
       730        740        750        760        770        780
acaattatca aggctatctc tgaggctttt ggtagaactg aggatcatgt taagaagcag
       790        800        810        820        830        840
aatacggaac tgggagactt gggacttgta gcaaaaggaa gccgttcgac tcagactatg
       850        860        870        880        890        900
atgttcaagc ccgaaccatt gactgttgtc aaggttttcg atacgtttcg acaaattgct
       910        920        930        940        950        960
aaggaaagtg gaaaagatag taatgaaaag aagaagaatc gaatgaaggc acttcttgtg
       970        980        990       1000       1010       1020
gcgacaacag actgtgaacc tctttactta acacgattac ttcaggcaaa attgcggtta
      1030       1040       1050       1060       1070       1080
gggttctcag gtcagactgt cttagccgca ttgggacaag ccgctgtata taatgaagag
      1090       1100       1110       1120       1130       1140
cactcaaagc cacccccaaa taccaagtct cctttagaag aggctgcaaa gattgttaaa
      1150       1160       1170       1180       1190       1200
caagtattca ctgtacttcc tgtctatgac attattgttc ctgctcttct aagtggcggt
      1210       1220       1230       1240       1250       1260
gtgtggaatc ttccaaaaac ttgtaacttt acattgggtg ttccaattgg acccatgctt
      1270       1280       1290       1300       1310       1320
gcaaaaccaa ccaaaggtgt agctgagata ctgaataaat tccaggacat cgttttcaca
      1330       1340       1350       1360       1370       1380
tgtgaataca aatatgatgg ggaacgtgca cagatacatt ttatggagga tggtacattt
      1390       1400       1410       1420       1430       1440
gagatataca gtcgaaatgc tgaaagaaac actgggaagt acccggatgt tgctcttgcg
      1450       1460       1470       1480       1490       1500
ttatcaagat taaagaagcc ttctgtgaaa tcttttattc tggattgtga agttgttgct
      1510       1520       1530       1540       1550       1560
ttcgacaggg agaaaaagaa aattcttccg ttccagattt tgagcactcg agcccgtaaa
      1570       1580       1590       1600       1610       1620
aatgtgaatg tcaatgatat caaggttggc gtatgcatct ttgcttttga catgttatat
      1630       1640       1650       1660       1670       1680
ctaaatggcc aacaacttat ccaggagaat cttaaaattc gccgagagaa gctatacgaa
      1690       1700       1710       1720       1730       1740
tcatttgagg aagatcctgg gtatttccag tttgcaactg ctgtaacatc taacgatatc
      1750       1760       1770       1780       1790       1800
gatgaaatac aaaagtttct tgacgcttct gttgatgttg gctgcgaagg tttaatcatt
      1810       1820       1830       1840       1850       1860
aaaacattgg actcagatgc tacctatgag cctgcaaagc gatcaaataa ttggctaaaa
      1870       1880       1890       1900       1910       1920
ctgaagaaag actatatgga cagcattggg gactctgtgg atcttgtacc aattgctgct
      1930       1940       1950       1960       1970       1980
ttccatggac gtggaaaacg cacaggtgtc tatggtgcat ttttactcgc ttgctatgat
      1990       2000       2010       2020       2030       2040
gttgataagg aagagttcca gagcatttgt aaaattggta ctggattttc tgatgccatg
      2050       2060       2070       2080       2090       2100
cttgacgagc ggtcgtccag tcttcgctct caagtgattg ctactccaaa acaatactac
      2110       2120       2130       2140       2150       2160
cgagttggag acagcctcaa tccagatgtt tggtttgagc ccactgaggt ctgggaagtg
      2170       2180       2190       2200       2210       2220
aaagcagctg acttaacaat tagccctgtg caccgtgcag ctactggcat agtggatcct
      2230       2240       2250       2260       2270       2280
gataagggga tttctcttcg ttttcctcgt ctacttcgtg tacgggaaga caagaagccc
      2290       2300       2310       2320       2330       2340
gaggaagcaa catcatctga gcagattgct gatttgtacc aagctcagaa acacaatcat
      2350       2360       2370
ccaagtaatg aagtcaaagg tgacgatgat tga  

Last modification date: Oct. 2, 2011