Homo sapiens L. (human) [HSA]

FULL NAME: Endonuclease 8-like 1


DESCRIPTION:
Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches.

STRUCTURE SIMILARITY:
Belongs to the FPG family.


CATALYTIC ACTIVITY:
Removes damaged bases from DNA, leaving an abasic site.
The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.


PROTEIN TYPE(S):
DNA N-glycosylase/AP-lyase
DNase
endonuclease
AP-lyase


RELATED PATHWAY(S):
base excision repair (BER)


RELATED DAMAGE:
DNA-psoralen
8-oxoG
Fapy-7meG
5-hydroxy C (5-OH-C)
5-hydroxy-U (5-OH-U)
Tg from Tg:G
8-oxoG from 8-oxoG:C
8-oxoG from 8-oxoG:G
8-oxoG from 8-oxoG:T
5,6-dihydro T (5,6-diH-T)
FapyA
FapyG
5,6-dihydroxy U (5,6-diOH-U)


Amino acids sequence

        10         20         30         40         50         60
MPEGPELHLA SQFVNEACRA LVFGGCVEKS SVSRNPEVPF ESSAYRISAS ARGKELRLIL
        70         80         90        100        110        120
SPLPGAQPQQ EPLALVFRFG MSGSFQLVPR EELPRHAHLR FYTAPPGPRL ALCFVDIRRF
       130        140        150        160        170        180
GRWDLGGKWQ PGRGPCVLQE YQQFRENVLR NLADKAFDRP ICEALLDQRF FNGIGNYLRA
       190        200        210        220        230        240
EILYRLKIPP FEKARSVLEA LQQHRPSPEL TLSQKIRTKL QNPDLLELCH SVPKEVVQLG
       250        260        270        280        290        300
GKGYGSESGE EDFAAFRAWL RCYGMPGMSS LQDRHGRTIW FQGDPGPLAP KGRKSRKKKS
       310        320        330        340        350        360
KATQLSPEDR VEDALPPSKA PSRTRRAKRD LPKRTATQRP EGTSLQQDPE APTVPKKGRR
       370        380        390
KGRQAASGHC RPRKVKADIP SLEPEGTSAS   

Encoded by NEIL1 gene

FULL NAME: nei endonuclease VIII-like 1 (E. coli)


OTHER NAME(S):
FLJ22402
FPG1
NEI1
hFPG1


DESCRIPTION:
NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).[supplied by OMIM, Mar 2008]


Nucleic acid sequence

        10         20         30         40         50         60
atgcctgagg gccccgagct gcacctggcc agccagtttg tgaatgaggc ctgcagggcg
        70         80         90        100        110        120
ctggtgttcg gcggctgcgt ggagaagtcc tctgtcagcc gcaaccctga ggtgcccttt
       130        140        150        160        170        180
gagagcagtg cctaccgcat ctcagcttca gcccgcggca aggagctgcg cctgatactg
       190        200        210        220        230        240
agccctctgc ctggggccca gccccaacag gagccactgg ccctggtctt ccgcttcggc
       250        260        270        280        290        300
atgtccggct cttttcagct ggtgccccgc gaggagctgc cacgccatgc ccacctgcgc
       310        320        330        340        350        360
ttttacacgg ccccgcctgg cccccggctc gccctatgtt tcgtggacat ccgccggttc
       370        380        390        400        410        420
ggccgctggg accttggggg aaagtggcag ccgggccgcg ggccctgtgt cttgcaggag
       430        440        450        460        470        480
taccagcagt tcagggagaa tgtgctacga aacctagcgg ataaggcctt tgaccggccc
       490        500        510        520        530        540
atctgcgagg ccctcctgga ccagaggttc ttcaatggca ttggcaacta tctgcgggca
       550        560        570        580        590        600
gagatcctgt accggctgaa gatccccccc tttgagaagg cccgctcggt cctggaggcc
       610        620        630        640        650        660
ctgcagcagc acaggccgag cccggagctg accctgagcc agaagataag gaccaagctg
       670        680        690        700        710        720
cagaatccag acctgctgga gctatgtcac tcagtgccca aggaagtggt ccagttgggg
       730        740        750        760        770        780
ggcaaaggct acgggtcaga gagcggggag gaggactttg ctgcctttcg agcctggctg
       790        800        810        820        830        840
cgctgctatg gcatgccagg catgagctcc ctgcaggacc ggcatggccg taccatctgg
       850        860        870        880        890        900
ttccaggggg atcctggacc gttggcaccc aaagggcgca agtcccgcaa aaagaaatcc
       910        920        930        940        950        960
aaggccacac agctgagtcc tgaggacaga gtggaggacg ctttgcctcc aagcaaggcc
       970        980        990       1000       1010       1020
ccttccagga cacgaagggc aaagagagac cttcctaaga ggactgcaac ccagcggcct
      1030       1040       1050       1060       1070       1080
gaggggacca gcctccagca ggacccagaa gctcccacag tgcccaagaa ggggaggagg
      1090       1100       1110       1120       1130       1140
aaggggcgac aggcagcctc tggccactgc agaccccgga aggtcaaggc tgacatccca
      1150       1160       1170
tccttggaac cagaggggac ctcagcctct tag  

Last modification date: Oct. 2, 2011