Homo sapiens L. (human) [HSA]

FULL NAME: Replication protein A 70 kDa DNA-binding subunit


DESCRIPTION:
Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.

STRUCTURE SIMILARITY:
Belongs to the replication factor A protein 1 family.


POST-TRANSLATIONAL MODIFICATION:
Phosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylated on lysine residues Lys-449 and Lys-577, with Lys-449 being the major site. Sumoylation promotes recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombinaison. Desumoylated by SENP6.


RELATED PATHWAY(S):
DNA replication
nucleotide excision repair (NER)
mismatch repair (MMR)
homologous recombination (HR)
Fanconi anemia (FA) pathway


Amino acids sequence

        10         20         30         40         50         60
MVGQLSEGAI AAIMQKGDTN IKPILQVINI RPITTGNSPP RYRLLMSDGL NTLSSFMLAT
        70         80         90        100        110        120
QLNPLVEEEQ LSSNCVCQIH RFIVNTLKDG RRVVILMELE VLKSAEAVGV KIGNPVPYNE
       130        140        150        160        170        180
GLGQPQVAPP APAASPAASS RPQPQNGSSG MGSTVSKAYG ASKTFGKAAG PSLSHTSGGT
       190        200        210        220        230        240
QSKVVPIASL TPYQSKWTIC ARVTNKSQIR TWSNSRGEGK LFSLELVDES GEIRATAFNE
       250        260        270        280        290        300
QVDKFFPLIE VNKVYYFSKG TLKIANKQFT AVKNDYEMTF NNETSVMPCE DDHHLPTVQF
       310        320        330        340        350        360
DFTGIDDLEN KSKDSLVDII GICKSYEDAT KITVRSNNRE VAKRNIYLMD TSGKVVTATL
       370        380        390        400        410        420
WGEDADKFDG SRQPVLAIKG ARVSDFGGRS LSVLSSSTII ANPDIPEAYK LRGWFDAEGQ
       430        440        450        460        470        480
ALDGVSISDL KSGGVGGSNT NWKTLYEVKS ENLGQGDKPD YFSSVATVVY LRKENCMYQA
       490        500        510        520        530        540
CPTQDCNKKV IDQQNGLYRC EKCDTEFPNF KYRMILSVNI ADFQENQWVT CFQESAEAIL
       550        560        570        580        590        600
GQNAAYLGEL KDKNEQAFEE VFQNANFRSF IFRVRVKVET YNDESRIKAT VMDVKPVDYR
       610
EYGRRLVMSI RRSALM    

Encoded by RPA1 gene

FULL NAME: replication protein A1, 70kDa


OTHER NAME(S):
HSSB
MST075
REPA1
RF-A
RP-A
RPA70


Nucleic acid sequence

        10         20         30         40         50         60
atggtcggcc aactgagcga gggggccatt gcggccatca tgcagaaggg ggatacaaac
        70         80         90        100        110        120
ataaagccca tcctccaagt catcaacatc cgtcccatta ctacggggaa tagtccgccg
       130        140        150        160        170        180
cgttatcgac tgctcatgag tgatggattg aacactctat cctctttcat gttggcgaca
       190        200        210        220        230        240
cagttgaacc ctctcgtgga ggaagaacaa ttgtccagca actgtgtatg ccagattcac
       250        260        270        280        290        300
agatttattg tgaacactct gaaagacgga aggagagtag ttatcttgat ggaattagaa
       310        320        330        340        350        360
gttttgaagt cagctgaagc agttggagtg aagattggca atccagtgcc ctataatgaa
       370        380        390        400        410        420
ggactcgggc agccgcaagt agctcctcca gcgccagcag ccagcccagc agcaagcagc
       430        440        450        460        470        480
aggccccagc cgcagaatgg aagctcggga atgggttcta ctgtttctaa ggcttatggt
       490        500        510        520        530        540
gcttcaaaga catttggaaa agctgcaggt cccagcctgt cacacacttc tgggggaaca
       550        560        570        580        590        600
cagtccaaag tggtgcccat tgccagcctc actccttacc agtccaagtg gaccatttgt
       610        620        630        640        650        660
gctcgtgtta ccaacaaaag tcagatccgt acctggagca actcccgagg ggaagggaag
       670        680        690        700        710        720
cttttctccc tagaactggt tgacgaaagt ggtgaaatcc gagctacagc tttcaatgag
       730        740        750        760        770        780
caagtggaca agttctttcc tcttattgaa gtgaacaagg tgtattattt ctcgaaaggc
       790        800        810        820        830        840
accctgaaga ttgctaacaa gcagttcaca gctgttaaaa atgactacga gatgaccttc
       850        860        870        880        890        900
aataacgaga cttccgtcat gccctgtgag gacgaccatc atttacctac ggttcagttt
       910        920        930        940        950        960
gatttcacgg ggattgatga cctcgagaac aagtcgaaag actcacttgt agacatcatc
       970        980        990       1000       1010       1020
gggatctgca agagctatga agacgccact aaaatcacag tgaggtctaa caacagagaa
      1030       1040       1050       1060       1070       1080
gttgccaaga ggaatatcta cttgatggac acatccggga aggtggtgac tgctacactg
      1090       1100       1110       1120       1130       1140
tggggggaag atgctgataa atttgatggt tctagacagc ccgtgttggc tatcaaagga
      1150       1160       1170       1180       1190       1200
gcccgagtct ctgatttcgg tggacggagc ctctccgtgc tgtcttcaag cactatcatt
      1210       1220       1230       1240       1250       1260
gcgaatcctg acatcccaga ggcctataag cttcgtggat ggtttgacgc agaaggacaa
      1270       1280       1290       1300       1310       1320
gccttagatg gtgtttccat ctctgatcta aagagcggcg gagtcggagg gagtaacacc
      1330       1340       1350       1360       1370       1380
aactggaaaa ccttgtatga ggtcaaatcc gagaacctgg gccaaggcga caagccggac
      1390       1400       1410       1420       1430       1440
tactttagtt ctgtggccac agtggtgtat cttcgcaaag agaactgcat gtaccaagcc
      1450       1460       1470       1480       1490       1500
tgcccgactc aggactgcaa taagaaagtg attgatcaac agaatggatt gtaccgctgt
      1510       1520       1530       1540       1550       1560
gagaagtgcg acaccgaatt tcccaatttc aagtaccgca tgatcctgtc agtaaatatt
      1570       1580       1590       1600       1610       1620
gcagattttc aagagaatca gtgggtgact tgtttccagg agtctgctga agctatcctt
      1630       1640       1650       1660       1670       1680
ggacaaaatg ctgcttatct tggggaatta aaagacaaga atgaacaggc atttgaagaa
      1690       1700       1710       1720       1730       1740
gttttccaga atgccaactt ccgatctttc atattcagag tcagggtcaa agtggagacc
      1750       1760       1770       1780       1790       1800
tacaacgacg agtctcgaat taaggccact gtgatggacg tgaagcccgt ggactacaga
      1810       1820       1830       1840       1850
gagtatggcc gaaggctggt catgagcatc aggagaagtg cattgatgtg a

Last modification date: Oct. 2, 2011