Drosophila melanogaster Meigen (fruit fly) [DME]

FULL NAME: Flap endonuclease 1


DESCRIPTION:
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA (By similarity).

STRUCTURE SIMILARITY:
Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.


POST-TRANSLATIONAL MODIFICATION:
Phosphorylated. Phosphorylation upon DNA damage induces relocalization to the nuclear plasma (By similarity).


RELATED PATHWAY(S):
DNA replication
base excision repair (BER)
non-homologous end-joining (NHEJ)


Amino acids sequence

        10         20         30         40         50         60
MGILGLSKLI ADLAPQAIRE SEMKHFFGRK VAIDASMCLY QFLIAVRSEG AQLATVNGDP
        70         80         90        100        110        120
TSHLMGMFYR TIRLLDNGIK PVYVFDGKPP DLKSGELAKR AERREEAEKA LKAATDAGDD
       130        140        150        160        170        180
AGIEKFNRRL VRVTKEHAKE AKELLTLMGV PYVDAPCEAE AQCAALVKAG KVYATATEDM
       190        200        210        220        230        240
DALTFGSTKL LRYLTYSEAR KMPVKEFSYD KLLEGLAINN REFIDLCILL GCDYCESIKG
       250        260        270        280        290        300
IGPKRAIELI NTYRDIETIL DNLDSSKYTV PENWNYKVAR ELFIEPEVAD ADSIDLKWVE
       310        320        330        340        350        360
PDEEGLVKFL CGDRQFNEER VRNGAKKLMK SKQAQTQVRL DSFFKTLPST PNATNAAKRK
       370        380
AEEAKKSANN KKAKTSGGGR GRRPK   

Encoded by Fen1 gene

FULL NAME: Flap endonuclease 1


OTHER NAME(S):
CG8648
Dmel\CG8648
DmFEN-1
EG:EG0003.3
FEN1


Nucleic acid sequence

        10         20         30         40         50         60
atgggaattt tgggcttatc taagctcatt gccgatctgg cgccacaggc gattcgcgaa
        70         80         90        100        110        120
agtgagatga agcatttttt cggtcgcaag gtagcaatcg atgctagtat gtgcctgtac
       130        140        150        160        170        180
cagttcctca tcgccgtgcg ctccgaaggc gcccagttgg ccaccgtaaa tggtgatccc
       190        200        210        220        230        240
acgtcccact taatgggcat gttctaccgc accatccgat tgctggacaa cggaatcaag
       250        260        270        280        290        300
ccggtatatg tcttcgacgg aaagccacca gatctcaagt ccggtgagct ggcgaagcgc
       310        320        330        340        350        360
gccgagcggc gggaggaagc ggagaaggca ctgaaggcgg ccaccgatgc gggagatgat
       370        380        390        400        410        420
gccggtatcg aaaagtttaa tcgccgattg gtccgggtaa cgaaggagca cgccaaagag
       430        440        450        460        470        480
gccaaggaac tgctcacact aatgggtgtg ccctatgttg atgcaccgtg cgaagcggag
       490        500        510        520        530        540
gcccagtgtg ccgctctggt gaaagctgga aaggtttatg ccaccgccac ggaggatatg
       550        560        570        580        590        600
gatgccctca cattcggatc tacaaaactg ttgagatacc ttacctacag cgaggcacga
       610        620        630        640        650        660
aagatgcccg tcaaggagtt cagctacgac aagctgttgg aaggtctggc cattaacaat
       670        680        690        700        710        720
cgagagttca ttgatctatg tattctgctg ggttgcgatt actgtgagag catcaagggt
       730        740        750        760        770        780
attggaccca agcgagcgat cgaactgatc aacacctatc gggatataga gactattctg
       790        800        810        820        830        840
gataacctgg actctagcaa atacaccgtg cccgagaact ggaactacaa ggtggcgcgg
       850        860        870        880        890        900
gaactcttca tcgaaccgga ggtagctgat gccgactcca tagatctcaa atgggtcgag
       910        920        930        940        950        960
ccggatgagg agggccttgt caagtttctc tgcggcgacc ggcagttcaa cgaagagcgc
       970        980        990       1000       1010       1020
gttcgcaacg gtgccaaaaa gctgatgaaa tccaagcagg cccagactca ggtgagactc
      1030       1040       1050       1060       1070       1080
gatagcttct ttaagacact gcccagcact ccgaatgcca caaacgctgc aaaacggaag
      1090       1100       1110       1120       1130       1140
gccgaggaag caaagaagag cgccaataac aaaaaggcca agaccagcgg cggtggacga
      1150
ggccggcgac ccaagtag    

Last modification date: Oct. 2, 2011