Schizo-saccharomyces pombe Lindner (fission yeast) [SPO]

FULL NAME: DNA topoisomerase 2


DESCRIPTION:
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.

MISCELLANEOUS:
Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.


STRUCTURE SIMILARITY:
Belongs to the type II topoisomerase family.


CATALYTIC ACTIVITY:
ATP-dependent breakage, passage and rejoining of double-stranded DNA.


POST-TRANSLATIONAL MODIFICATION:
Phosphorylated at multiple sites at both extremities of the protein.


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MSIDADFSDY EDEASGDENV LPNTTTKRKA STTSSKSRAK KASTPDLRQT SLTSMTASEQ
        70         80         90        100        110        120
IPLVTNNGNG NSNVSTQYQR LTPREHVLRR PDTYIGSIEP TTSEMWVFDS EKNKLDYKAV
       130        140        150        160        170        180
TYVPGLYKIF DEIIVNAADN KVRDPNMNTL KVTLDPEANV ISIYNNGKGI PIEIHDKEKI
       190        200        210        220        230        240
YIPELIFGNL LTSSNYDDNQ KKVTGGRNGY GAKLCNIFST EFVVETADKE RMKKYKQTWY
       250        260        270        280        290        300
DNMSRKSEPV ITSLKKPDEY TKITFKPDLA KFGMDKIDDD MVSIIKRRIY DMAGTVRETK
       310        320        330        340        350        360
VYLNNERISI SGFKKYVEMY LASDTKPDEE PPRVIYEHVN DRWDVAFAVS DGQFKQVSFV
       370        380        390        400        410        420
NNISTIRGGT HVNYVANKIV DAIDEVVKKE NKKAPVKAFQ IKNYVQVFVN CQIENPSFDS
       430        440        450        460        470        480
QTKETLTTKV SAFGSQCTLS DKFLKAIKKS SVVEEVLKFA TAKADQQLSK GDGGLRSRIT
       490        500        510        520        530        540
GLTKLEDANK AGTKESHKCV LILTEGDSAK SLAVSGLSVV GRDYYGVFPL RGKLLNVREA
       550        560        570        580        590        600
SHSQILNNKE IQAIKKIMGF THKKTYTDVK GLRYGHLMIM TDQDHDGSHI KGLIINYLES
       610        620        630        640        650        660
SYPSLLQIPG FLIQFITPII KCTRGNQVQA FYTLPEYEYW KEANNNGRGW KIKYYKGLGT
       670        680        690        700        710        720
SDHDDMKSYF SDLDRHMKYF HAMQEKDAEL IEMAFAKKKA DVRKEWLRTY RPGIYMDYTQ
       730        740        750        760        770        780
PQIPIDDFIN RELIQFSMAD NIRSIPSVVD GLKPGQRKVV YYCFKRNLVH ETKVSRLAGY
       790        800        810        820        830        840
VASETAYHHG EVSMEQTIVN LAQNFVGSNN INLLMPNGQF GTRSEGGKNA SASRYLNTAL
       850        860        870        880        890        900
SPLARVLFNS NDDQLLNYQN DEGQWIEPEY YVPILPMVLV NGAEGIGTGW STFIPNYNPK
       910        920        930        940        950        960
DITANLRHML NGEPLEIMTP WYRGFRGSIT KVAPDRYKIS GIINQIGENK VEITELPIRF
       970        980        990       1000       1010       1020
WTQDMKEYLE AGLVGTEKIR KFIVDYESHH GEGNVHFNVT LTEAGMKEAL NESLEVKFKL
      1030       1040       1050       1060       1070       1080
SRTQATSNMI AFDASGRIKK YDSVEDILTE FYEVRLRTYQ RRKEHMVNEL EKRFDRFSNQ
      1090       1100       1110       1120       1130       1140
ARFIHMIIEG ELVVSKKKKK DLIVELKEKK FQPISKPKKG HLVDLEVENA LAEEEQSGDV
      1150       1160       1170       1180       1190       1200
SQDEDSDAYN YLLSMPLWSL TYERYVELLK KKDEVMAELD ALIKKTPKEL WLHDLDAFEH
      1210       1220       1230       1240       1250       1260
AWNKVMDDIQ REMLEEEQSS RDFVNRTKKK PRGKSTGTRK PRAIAGSSSS TAVKKEASSE
      1270       1280       1290       1300       1310       1320
SKPSTTNRKQ QTLLEFAASK EPEKSSDINI VKTEDNSHGL SVEENRISKS PGLDSSDSGK
      1330       1340       1350       1360       1370       1380
SRKRSQSVDS EDAGSKKPVK KIAASASGRG RKTNKPVATT IFSSDDEDDL LPSSLKPSTI
      1390       1400       1410       1420       1430       1440
TSTKASAKNK GKKASSVKKQ SPEDDDDDFI IPGSSSTPKA SSTNAEPPED SDSPIRKRPT
      1450       1460       1470       1480
RRAAATVKTP IYVDPSFDSM DEPSMQDDSF IVDNDEDVDD YDESD 

Encoded by top2 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgtccattg atgcggattt ctccgactat gaagatgaag cgtctggaga cgaaaacgtt
        70         80         90        100        110        120
ttacctaaca caaccaccaa aaggaaagca tctacaacat cctcaaagtc tcgcgccaag
       130        140        150        160        170        180
aaggcaagca ctcctgactt aaggcaaact agcttgacat ccatgacagc ttctgaacag
       190        200        210        220        230        240
ataccactag ttaccaacaa cggaaatgga aattctaatg tatctacaca gtaccagcgt
       250        260        270        280        290        300
cttacaccga gagagcatgt gctaagacgt ccggatacat acattggcag tattgagcca
       310        320        330        340        350        360
acgacttctg aaatgtgggt ctttgactct gagaagaaca agctggatta caaagcagtg
       370        380        390        400        410        420
acctatgttc caggccttta caagatattt gacgaaataa ttgtcaatgc tgctgacaac
       430        440        450        460        470        480
aaagttcgcg acccaaacat gaatacccta aaggtgactt tggatcctga agcaaatgtc
       490        500        510        520        530        540
atatcaattt ataacaatgg caagggcatt cctatagaga ttcatgataa agagaaaatt
       550        560        570        580        590        600
tacatccccg agcttatttt tggtaacttg cttacttcaa gcaattacga cgataatcaa
       610        620        630        640        650        660
aagaaggtaa caggaggaag aaatggttac ggcgcaaaac tttgcaatat tttttctact
       670        680        690        700        710        720
gaatttgtcg ttgagacagc tgataaggaa aggatgaaga aatataaaca aacttggtat
       730        740        750        760        770        780
gacaatatgt ccagaaagtc tgagccagtg atcacttctc tcaagaaacc cgatgaatac
       790        800        810        820        830        840
acaaaaatta cattcaaacc cgatttagca aaatttggta tggacaagat tgatgatgat
       850        860        870        880        890        900
atggtatcta ttataaaacg ccgtatttat gatatggcag gtaccgttcg tgaaactaaa
       910        920        930        940        950        960
gtttacctga acaatgaacg catcagtata agtggattta agaaatatgt ggagatgtat
       970        980        990       1000       1010       1020
cttgcttctg acactaaacc tgatgaagag cctcctaggg taatttatga acatgttaat
      1030       1040       1050       1060       1070       1080
gaccgttggg acgtggcctt tgctgtttca gatggacagt ttaagcaggt ttcgtttgtt
      1090       1100       1110       1120       1130       1140
aacaatattt ctaccattcg tggaggtaca catgttaatt atgttgccaa taaaattgtc
      1150       1160       1170       1180       1190       1200
gatgctatag atgaagtcgt caagaaggag aataaaaagg cacccgttaa agcgtttcaa
      1210       1220       1230       1240       1250       1260
ataaaaaatt acgtacaggt atttgtcaat tgtcaaatag agaacccatc ctttgattcc
      1270       1280       1290       1300       1310       1320
caaacaaagg agactctcac cacgaaagtt tctgcttttg gctctcaatg cacattaagt
      1330       1340       1350       1360       1370       1380
gataaatttt taaaagctat caaaaaatct tctgtagtgg aggaggtttt aaaatttgct
      1390       1400       1410       1420       1430       1440
accgctaagg cagatcagca actcagtaag ggcgatgggg gtttgcgttc tcgtataacg
      1450       1460       1470       1480       1490       1500
ggtttaacca aactcgaaga cgcaaataaa gcgggtacca aagaatccca taagtgtgtc
      1510       1520       1530       1540       1550       1560
cttatcctca ctgagggaga ttctgctaaa tctttagctg tttcgggtct cagtgttgtt
      1570       1580       1590       1600       1610       1620
ggtagagact attatggagt ttttccgtta agaggaaaac tactgaatgt acgggaggct
      1630       1640       1650       1660       1670       1680
tctcattccc aaatcctaaa taacaaagaa attcaagcta tcaagaaaat catgggattc
      1690       1700       1710       1720       1730       1740
actcataaaa aaacttatac cgatgtgaaa ggtcttcgtt atggtcattt aatgattatg
      1750       1760       1770       1780       1790       1800
acagatcaag atcatgatgg atcacatatt aaaggtttaa ttattaatta tttagaatcc
      1810       1820       1830       1840       1850       1860
tcgtatcctt ctcttttgca aattcctgga ttcttgatcc aatttatcac acctatcatt
      1870       1880       1890       1900       1910       1920
aagtgcactc gtggcaatca ggttcaagca ttttacactt tacccgagta cgaatactgg
      1930       1940       1950       1960       1970       1980
aaggaagcta ataacaatgg acgtggttgg aaaattaaat actacaaggg tttgggaaca
      1990       2000       2010       2020       2030       2040
agtgaccatg acgatatgaa aagttatttt tcagaccttg atcgtcacat gaagtacttt
      2050       2060       2070       2080       2090       2100
catgccatgc aggagaagga tgctgaatta attgaaatgg catttgccaa gaaaaaggcc
      2110       2120       2130       2140       2150       2160
gatgtgcgta aggaatggct tagaacgtat cgacctggta tatatatgga ttatactcaa
      2170       2180       2190       2200       2210       2220
cctcagattc cgattgatga cttcattaat cgagagctta tccaattcag tatggctgat
      2230       2240       2250       2260       2270       2280
aatatccgtt cgattccttc agtagtagat ggcttgaagc ctggtcagcg taaagttgtc
      2290       2300       2310       2320       2330       2340
tattattgtt ttaaacgcaa tctcgtccat gaaactaaag tcagtagact tgccggctat
      2350       2360       2370       2380       2390       2400
gttgctagtg aaactgcata ccaccatggc gaggtttcga tggagcaaac tatagttaat
      2410       2420       2430       2440       2450       2460
cttgcccaaa attttgttgg cagtaacaac attaatttgc tgatgcctaa tggacaattc
      2470       2480       2490       2500       2510       2520
ggtacacgat ctgagggtgg aaaaaatgca tcggcttcaa ggtatctaaa tacagcttta
      2530       2540       2550       2560       2570       2580
tcacccttag cacgtgtttt atttaattcc aatgatgacc agcttcttaa ttaccaaaac
      2590       2600       2610       2620       2630       2640
gacgaaggcc agtggattga accagagtat tatgtgccaa ttcttcccat ggtacttgtc
      2650       2660       2670       2680       2690       2700
aatggagccg aaggtattgg tactggctgg tctactttta ttcctaatta taaccctaag
      2710       2720       2730       2740       2750       2760
gatataactg ctaatttaag acatatgctt aatggcgagc ctttggaaat tatgactccc
      2770       2780       2790       2800       2810       2820
tggtaccgcg gctttcgtgg aagtataacg aaagttgcgc ctgacaggta caaaatatct
      2830       2840       2850       2860       2870       2880
ggtataatta accaaattgg tgaaaacaaa gtagaaatta ctgaattgcc tatacgattt
      2890       2900       2910       2920       2930       2940
tggactcagg atatgaagga gtatctggag gctggtcttg ttggtacgga aaaaattcgt
      2950       2960       2970       2980       2990       3000
aaattcatcg tggactatga aagccatcac ggtgaaggaa atgttcactt taatgtcacg
      3010       3020       3030       3040       3050       3060
cttaccgaag ctggcatgaa agaagcatta aatgaatcct tggaagttaa gtttaaactg
      3070       3080       3090       3100       3110       3120
tcgcgtactc aagcaacgag taatatgatt gcttttgatg catctggacg gatcaagaag
      3130       3140       3150       3160       3170       3180
tatgacagtg ttgaagatat tttgaccgag ttttacgaag tacgtttaag gacataccag
      3190       3200       3210       3220       3230       3240
agacgtaaag agcatatggt taatgaattg gaaaaaaggt ttgatagatt ttcaaatcaa
      3250       3260       3270       3280       3290       3300
gcgcggttta ttcatatgat tatagagggg gagcttgtag tttcaaaaaa gaagaaaaag
      3310       3320       3330       3340       3350       3360
gatctcattg tggagctgaa ggaaaagaag tttcaaccta tcagtaaacc aaagaaaggg
      3370       3380       3390       3400       3410       3420
catttggttg atttagaagt tgaaaatgct cttgctgaag aagaacaatc tggtgatgtt
      3430       3440       3450       3460       3470       3480
tcgcaagacg aggattcaga tgcctacaat taccttcttt caatgccttt atggtctttg
      3490       3500       3510       3520       3530       3540
acctatgaac ggtatgtgga acttctcaag aaaaaggacg aagtaatggc cgaactggat
      3550       3560       3570       3580       3590       3600
gctttgatta aaaaaactcc taaagaattg tggcttcatg atttggatgc atttgagcat
      3610       3620       3630       3640       3650       3660
gcttggaata aggttatgga tgatattcag agagaaatgt tagaagaaga gcaatcctct
      3670       3680       3690       3700       3710       3720
agagattttg taaatcgaac gaaaaagaag cctcgaggta aatccactgg tactaggaaa
      3730       3740       3750       3760       3770       3780
cccagggcta ttgccggatc ctcctcaagt actgctgtta aaaaagaagc tagcagcgaa
      3790       3800       3810       3820       3830       3840
tccaaaccat ctacaacaaa tcgaaagcaa caaactttac ttgagttcgc agcgtcgaaa
      3850       3860       3870       3880       3890       3900
gaacctgaga aatcatctga cataaacatt gtcaagacag aggataactc tcatggctta
      3910       3920       3930       3940       3950       3960
tcagttgaag aaaatcgcat atctaagagc ccgggattgg atagcagtga tagtggaaaa
      3970       3980       3990       4000       4010       4020
tcaagaaaaa gaagtcaatc tgttgactct gaagacgcag gtagtaaaaa acccgtcaaa
      4030       4040       4050       4060       4070       4080
aaaatagcag cctcggcgtc tggaagagga agaaagacta ataaaccagt tgctactacc
      4090       4100       4110       4120       4130       4140
atattttctt cagacgatga agatgatttg ttgcctagca gtttgaaacc ttccaccata
      4150       4160       4170       4180       4190       4200
acatccacga aggcttctgc gaagaataaa gggaaaaaag caagctcggt aaagaaacaa
      4210       4220       4230       4240       4250       4260
tcacctgaag atgacgatga tgattttatt attccaggta gtagttcaac tccaaaagct
      4270       4280       4290       4300       4310       4320
agttcgacta acgcagagcc accagaagat tcagattctc cgattagaaa gaggccaacc
      4330       4340       4350       4360       4370       4380
agaagagcag cggctacagt gaaaacacct atttacgttg atccctcgtt tgatagcatg
      4390       4400       4410       4420       4430       4440
gatgaaccca gcatgcaaga tgattctttt atcgtcgata acgatgagga tgtagacgat
      4450
tatgatgaga gtgattag    

Last modification date: Oct. 2, 2011