Saccharomyces cerevisiae Meyen ex E.C. Hansen (budding yeast) [SCE]

FULL NAME: DNA topoisomerase 2


DESCRIPTION:
Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks. Essential during mitosis and meiosis for proper segregation of daughter chromosomes.

MISCELLANEOUS:
Present with 5730 molecules/cell in log phase SD medium.
Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.
In yeast topoisomerase II can substitute topoisomerase I for the relaxing activity.


STRUCTURE SIMILARITY:
Belongs to the type II topoisomerase family.


CATALYTIC ACTIVITY:
ATP-dependent breakage, passage and rejoining of double-stranded DNA.


POST-TRANSLATIONAL MODIFICATION:
Phosphorylation enhances the activity. Stimulates decatenation activity.


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MSTEPVSASD KYQKISQLEH ILKRPDTYIG SVETQEQLQW IYDEETDCMI EKNVTIVPGL
        70         80         90        100        110        120
FKIFDEILVN AADNKVRDPS MKRIDVNIHA EEHTIEVKND GKGIPIEIHN KENIYIPEMI
       130        140        150        160        170        180
FGHLLTSSNY DDDEKKVTGG RNGYGAKLCN IFSTEFILET ADLNVGQKYV QKWENNMSIC
       190        200        210        220        230        240
HPPKITSYKK GPSYTKVTFK PDLTRFGMKE LDNDILGVMR RRVYDINGSV RDINVYLNGK
       250        260        270        280        290        300
SLKIRNFKNY VELYLKSLEK KRQLDNGEDG AAKSDIPTIL YERINNRWEV AFAVSDISFQ
       310        320        330        340        350        360
QISFVNSIAT TMGGTHVNYI TDQIVKKISE ILKKKKKKSV KSFQIKNNMF IFINCLIENP
       370        380        390        400        410        420
AFTSQTKEQL TTRVKDFGSR CEIPLEYINK IMKTDLATRM FEIADANEEN ALKKSDGTRK
       430        440        450        460        470        480
SRITNYPKLE DANKAGTKEG YKCTLVLTEG DSALSLAVAG LAVVGRDYYG CYPLRGKMLN
       490        500        510        520        530        540
VREASADQIL KNAEIQAIKK IMGLQHRKKY EDTKSLRYGH LMIMTDQDHD GSHIKGLIIN
       550        560        570        580        590        600
FLESSFPGLL DIQGFLLEFI TPIIKVSITK PTKNTIAFYN MPDYEKWREE ESHKFTWKQK
       610        620        630        640        650        660
YYKGLGTSLA QEVREYFSNL DRHLKIFHSL QGNDKDYIDL AFSKKKADDR KEWLRQYEPG
       670        680        690        700        710        720
TVLDPTLKEI PISDFINKEL ILFSLADNIR SIPNVLDGFK PGQRKVLYGC FKKNLKSELK
       730        740        750        760        770        780
VAQLAPYVSE CTAYHHGEQS LAQTIIGLAQ NFVGSNNIYL LLPNGAFGTR ATGGKDAAAA
       790        800        810        820        830        840
RYIYTELNKL TRKIFHPADD PLYKYIQEDE KTVEPEWYLP ILPMILVNGA EGIGTGWSTY
       850        860        870        880        890        900
IPPFNPLEII KNIRHLMNDE ELEQMHPWFR GWTGTIEEIE PLRYRMYGRI EQIGDNVLEI
       910        920        930        940        950        960
TELPARTWTS TIKEYLLLGL SGNDKIKPWI KDMEEQHDDN IKFIITLSPE EMAKTRKIGF
       970        980        990       1000       1010       1020
YERFKLISPI SLMNMVAFDP HGKIKKYNSV NEILSEFYYV RLEYYQKRKD HMSERLQWEV
      1030       1040       1050       1060       1070       1080
EKYSFQVKFI KMIIEKELTV TNKPRNAIIQ ELENLGFPRF NKEGKPYYGS PNDEIAEQIN
      1090       1100       1110       1120       1130       1140
DVKGATSDEE DEESSHEDTE NVINGPEELY GTYEYLLGMR IWSLTKERYQ KLLKQKQEKE
      1150       1160       1170       1180       1190       1200
TELENLLKLS AKDIWNTDLK AFEVGYQEFL QRDAEARGGN VPNKGSKTKG KGKRKLVDDE
      1210       1220       1230       1240       1250       1260
DYDPSKKNKK STARKGKKIK LEDKNFERIL LEQKLVTKSK APTKIKKEKT PSVSETKTEE
      1270       1280       1290       1300       1310       1320
EENAPSSTSS SSIFDIKKED KDEGELSKIS NKFKKISTIF DKMGSTSATS KENTPEQDDV
      1330       1340       1350       1360       1370       1380
ATKKNQTTAK KTAVKPKLAK KPVRKQQKVV ELSGESDLEI LDSYTDREDS NKDEDDAIPQ
      1390       1400       1410       1420
RSRRQRSSRA ASVPKKSYVE TLELSDDSFI EDDEEENQGS DVSFNEED 

Encoded by TOP2 gene


OTHER NAME(S):
TOR3
TRF3


Nucleic acid sequence

        10         20         30         40         50         60
atgtcaactg aaccggtaag cgcctctgat aaatatcaga aaatttctca actggaacat
        70         80         90        100        110        120
atcttaaaaa gaccagacac ttatatcggt tctgttgaaa ctcaagagca gctgcaatgg
       130        140        150        160        170        180
atatacgatg aagagaccga ttgcatgatt gaaaaaaatg tcacaattgt accagggttg
       190        200        210        220        230        240
ttcaaaatct ttgatgaaat cttagtcaat gcggcagata ataaagttcg tgatccatcg
       250        260        270        280        290        300
atgaaacgaa tcgatgtaaa catacatgct gaggaacata ctatagaagt gaaaaatgat
       310        320        330        340        350        360
ggaaaaggta ttcccataga gattcataac aaggagaata tttatattcc tgaaatgata
       370        380        390        400        410        420
tttggtcatt tgttgacatc atccaattat gatgatgatg agaagaaagt cactggtggt
       430        440        450        460        470        480
agaaacggtt atggtgctaa gctttgtaat atattttcca ctgaattcat attagaaact
       490        500        510        520        530        540
gcagatctaa atgttggcca gaaatatgtt caaaaatggg aaaataacat gagcatttgc
       550        560        570        580        590        600
caccccccaa aaataacatc ttacaagaag ggtccatcat atacaaaggt gacatttaag
       610        620        630        640        650        660
ccggatttaa ccagattcgg aatgaaagag ctagataatg atatcttagg agtgatgcga
       670        680        690        700        710        720
agaagagttt atgatatcaa tggttctgtt cgtgacatta atgtctatct gaatggcaag
       730        740        750        760        770        780
tccttaaaga taagaaattt caaaaattat gttgaactct acttgaaatc actcgaaaaa
       790        800        810        820        830        840
aaaagacaac tagataacgg tgaggacggt gccgctaagt ctgatatccc gactattctt
       850        860        870        880        890        900
tatgagagaa taaacaacag atgggaagtt gcttttgcgg tttctgatat ctcttttcaa
       910        920        930        940        950        960
caaatttctt ttgtgaattc cattgcaact accatgggtg gtacccatgt caattacata
       970        980        990       1000       1010       1020
acagaccaaa ttgtaaaaaa aatttcagaa attttgaaga aaaagaagaa aaaaagtgtg
      1030       1040       1050       1060       1070       1080
aagtcttttc agattaaaaa taatatgttc attttcatta attgtttgat tgagaatcct
      1090       1100       1110       1120       1130       1140
gcatttacct cacaaacaaa agagcaactg acaacaagag tcaaagattt tgggtcccgt
      1150       1160       1170       1180       1190       1200
tgtgagattc ctcttgaata tattaataag attatgaaaa ctgatttggc tacaagaatg
      1210       1220       1230       1240       1250       1260
tttgaaattg ccgacgcaaa tgaagaaaat gcgctaaaga agtctgatgg tacaaggaaa
      1270       1280       1290       1300       1310       1320
agcagaatta ctaattaccc taaactggaa gatgccaaca aagccggtac aaaagaaggc
      1330       1340       1350       1360       1370       1380
tataaatgta ctttagttct gacagaaggg gattccgcct tgtcattagc tgttgcaggt
      1390       1400       1410       1420       1430       1440
ttagctgttg ttggtagaga ttattatggt tgttatccac ttcgtggtaa aatgctgaat
      1450       1460       1470       1480       1490       1500
gttagagaag ctagtgctga tcagatacta aaaaacgcgg aaattcaagc cattaaaaaa
      1510       1520       1530       1540       1550       1560
attatggggt tacaacatcg caagaaatat gaagatacaa aatctttaag atatgggcat
      1570       1580       1590       1600       1610       1620
cttatgatca tgaccgatca agatcatgat ggttcgcata ttaaaggttt aattataaac
      1630       1640       1650       1660       1670       1680
tttttagaaa gctcatttcc tggtcttttg gatatccaag gtttcttact tgaattcata
      1690       1700       1710       1720       1730       1740
actccgatca tcaaagtttc catcactaaa ccaacaaaaa acactattgc attctacaat
      1750       1760       1770       1780       1790       1800
atgccggact atgaaaaatg gagagaggaa gaatcgcaca aatttacttg gaagcagaag
      1810       1820       1830       1840       1850       1860
tattataaag gattagggac ttctctagca caagaagtcc gagaatattt ttcgaacttg
      1870       1880       1890       1900       1910       1920
gacagacatt tgaaaatatt ccattctttg cagggtaatg ataaagatta cattgattta
      1930       1940       1950       1960       1970       1980
gctttctcca agaaaaaggc agatgaccgt aaagaatggc tgagacaata cgaacctggt
      1990       2000       2010       2020       2030       2040
actgttttag accctacttt aaaagagatt ccaattagcg acttcattaa taaggaatta
      2050       2060       2070       2080       2090       2100
atcctttttt ctttggccga taatatacgg tcgattccca atgttttaga tggatttaaa
      2110       2120       2130       2140       2150       2160
cctggccaaa gaaaagttct ttatggttgt ttcaaaaaaa atttaaagtc ggaactgaaa
      2170       2180       2190       2200       2210       2220
gtagctcaac ttgcaccata cgtgagcgaa tgtacggcat atcaccatgg tgagcagtca
      2230       2240       2250       2260       2270       2280
ttggcacaaa ctattattgg gctagcccaa aactttgttg ggtccaacaa tatttacttg
      2290       2300       2310       2320       2330       2340
ctattaccta acggtgcttt cggtacaaga gccactggtg gtaaagatgc agcggcagcg
      2350       2360       2370       2380       2390       2400
agatatatct acacagaatt gaacaaatta actcgtaaga tatttcaccc tgctgatgat
      2410       2420       2430       2440       2450       2460
ccattataca aatatataca agaagatgag aaaacagtgg agccagagtg gtatttacca
      2470       2480       2490       2500       2510       2520
attcttccta tgattcttgt taacggtgct gagggtattg gcactggctg gagtacttac
      2530       2540       2550       2560       2570       2580
attcctccat tcaacccatt ggaaattata aagaatataa gacatttaat gaacgacgag
      2590       2600       2610       2620       2630       2640
gagcttgagc aaatgcatcc gtggtttagg ggatggaccg gtactattga agaaattgag
      2650       2660       2670       2680       2690       2700
cctctgcgtt acagaatgta cggtaggatt gaacaaattg gagataacgt cttagaaata
      2710       2720       2730       2740       2750       2760
actgagttgc cagccagaac ttggacatcg actataaagg agtacttact tttaggttta
      2770       2780       2790       2800       2810       2820
agcggtaacg ataaaataaa accctggatc aaagatatgg aggagcagca cgatgataac
      2830       2840       2850       2860       2870       2880
atcaaattca taatcacgct atcacctgag gaaatggcta aaacaaggaa aataggtttt
      2890       2900       2910       2920       2930       2940
tatgaaagat ttaaactaat ttcgcctata agtttgatga atatggtcgc atttgatcct
      2950       2960       2970       2980       2990       3000
cacgggaaaa tcaagaagta caattccgtg aatgaaatat taagcgaatt ttactacgtc
      3010       3020       3030       3040       3050       3060
agactagaat actatcaaaa aagaaaagac catatgagcg aaaggttaca gtgggaggta
      3070       3080       3090       3100       3110       3120
gagaaatact ctttccaagt aaaatttatt aaaatgatta ttgaaaagga gttaacagtc
      3130       3140       3150       3160       3170       3180
accaataagc ctaggaacgc tattatccaa gaacttgaga atttagggtt ccccagattt
      3190       3200       3210       3220       3230       3240
aataaggaag gtaaaccata ttatggaagt cctaacgatg agatagctga acaaattaac
      3250       3260       3270       3280       3290       3300
gacgtaaaag gcgcaacttc tgatgaagaa gatgaagaaa gttcacacga agatactgaa
      3310       3320       3330       3340       3350       3360
aatgttataa atggtcctga agaactatat ggcacatatg aatatttatt aggaatgaga
      3370       3380       3390       3400       3410       3420
atatggtcat tgaccaagga aagatatcaa aagctgttga aacaaaaaca agaaaaggag
      3430       3440       3450       3460       3470       3480
acagagttgg aaaacttgtt aaaactttcc gcgaaagata tatggaacac tgacttgaag
      3490       3500       3510       3520       3530       3540
gcttttgagg tgggatatca agaatttttg caacgagatg cagaagctcg cggtggtaat
      3550       3560       3570       3580       3590       3600
gttcccaata aagggagcaa aacgaaaggt aaaggaaaaa gaaagcttgt tgacgacgaa
      3610       3620       3630       3640       3650       3660
gactacgacc catcaaaaaa aaacaagaaa agtactgcta gaaagggcaa aaaaattaag
      3670       3680       3690       3700       3710       3720
ttagaggata agaattttga aaggattttg ttagaacaaa aactagtaac caaaagcaag
      3730       3740       3750       3760       3770       3780
gcgcctacaa agattaaaaa agagaaaacg ccttctgttt cagaaacaaa aacagaagaa
      3790       3800       3810       3820       3830       3840
gaagagaatg ctccttcttc cacgagttct tcttctattt tcgacataaa gaaagaagat
      3850       3860       3870       3880       3890       3900
aaagatgagg gcgaactgag taagatttcg aacaagttta aaaaaattag cacgattttt
      3910       3920       3930       3940       3950       3960
gacaaaatgg gttctacttc cgctacatcg aaggaaaata caccagaaca ggacgatgta
      3970       3980       3990       4000       4010       4020
gccactaaaa aaaatcaaac aaccgctaaa aaaacagctg taaaacctaa attggccaag
      4030       4040       4050       4060       4070       4080
aagccagtca ggaaacaaca aaaagttgtg gaactatctg gtgaaagcga cctagaaatt
      4090       4100       4110       4120       4130       4140
ttagattcat acactgatcg ggaagatagc aataaagatg aagatgatgc tataccacaa
      4150       4160       4170       4180       4190       4200
cgatcaagga gacaaagatc gtcgagagct gcgtcggttc ctaagaaatc ttacgttgaa
      4210       4220       4230       4240       4250       4260
actttagaat tatctgacga cagtttcatc gaagatgatg aagaggaaaa ccaaggatca
      4270       4280
gatgtttcgt tcaatgaaga ggattga   

Last modification date: Oct. 2, 2011