Mus musculus L. (mouse) [MMU]

FULL NAME: DNA topoisomerase 1


DESCRIPTION:
The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.

MISCELLANEOUS:
When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.


STRUCTURE SIMILARITY:
Belongs to the eukaryotic type I topoisomerase family.


CATALYTIC ACTIVITY:
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.


POST-TRANSLATIONAL MODIFICATION:
Sumoylated. Lys-119 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment (By similarity).


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MSGDHLHNDS QIEADFRLND SHKHKDKHKD REHRHKEHKK DKDKDREKSK HSNSEHKDSE
        70         80         90        100        110        120
KKHKEKEKTK HKDGSSEKHK DKHKDRDKER RKEEKIRAAG DAKIKKEKEN GFSSPPRIKD
       130        140        150        160        170        180
EPEDDGYFAP PKEDIKPLKR LRDEDDADYK PKKIKTEDIK KEKKRKSEEE EDGKLKKPKN
       190        200        210        220        230        240
KDKDKKVAEP DNKKKKPKKE EEQKWKWWEE ERYPEGIKWK FLEHKGPVFA PPYEPLPESV
       250        260        270        280        290        300
KFYYDGKVMK LSPKAEEVAT FFAKMLDHEY TTKEIFRKNF FKDWRKEMTN DEKNTITNLS
       310        320        330        340        350        360
KCDFTQMSQY FKAQSEARKQ MSKEEKLKIK EENEKLLKEY GFCVMDNHRE RIANFKIEPP
       370        380        390        400        410        420
GLFRGRGNHP KMGMLKRRIM PEDIIINCSK DAKVPSPPPG HKWKEVRHDN KVTWLVSWTE
       430        440        450        460        470        480
NIQGSIKYIM LNPSSRIKGE KDWQKYETAR RLKKCVDKIR NQYREDWKSK EMKVRQRAVA
       490        500        510        520        530        540
LYFIDKLALR AGNEKEEGET ADTVGCCSLR VEHINLHPEL DGQEYVVEFD FPGKDSIRYY
       550        560        570        580        590        600
NKVPVEKRVF KNLQLFMENK QPEDDLFDRL NTGILNKHLQ DLMEGLTAKV FRTYNASITL
       610        620        630        640        650        660
QQQLKELTAP DENVPAKILS YNRANRAVAI LCNHQRAPPK TFEKSMMNLQ SKIDAKKDQL
       670        680        690        700        710        720
ADARRDLKSA KADAKVMKDA KTKKVVESKK KAVQRLEEQL MKLEVQATDR EENKQIALGT
       730        740        750        760
SKLNYLDPRI TVAWCKKWGV PIEKIYNKTQ REKFAWAIDM TDEDYEF 

Encoded by Top1 gene

FULL NAME: topoisomerase (DNA) I


OTHER NAME(S):
AI467334
D130064I21Rik
Top-1


Nucleic acid sequence

        10         20         30         40         50         60
atgagcgggg accatctcca caacgattcc cagatcgaag cggatttccg attgaatgat
        70         80         90        100        110        120
tctcacaaac acaaagataa acacaaagat cgagaacacc ggcataagga gcacaagaag
       130        140        150        160        170        180
gataaggata aggaccggga aaagtctaag catagcaaca gtgaacataa agattctgaa
       190        200        210        220        230        240
aagaaacaca aagagaaaga gaaaaccaaa cacaaagatg gcagctcaga aaaacataaa
       250        260        270        280        290        300
gacaaacata aagacagaga caaggaaaga cgaaaggagg aaaagattag agctgctggg
       310        320        330        340        350        360
gatgcaaaaa taaagaagga aaaggaaaat ggcttctcta gtccgccacg aattaaagat
       370        380        390        400        410        420
gaacctgagg atgatggcta ttttgctcct ccaaaagagg atattaagcc attgaagagg
       430        440        450        460        470        480
cttcgagatg aagatgatgc tgattataaa cctaagaaaa ttaagacaga agatatcaag
       490        500        510        520        530        540
aaggagaaga aaagaaaatc agaagaggaa gaggatggta aacttaaaaa gcccaagaat
       550        560        570        580        590        600
aaagataaag ataaaaaagt tgctgagcca gataataaga aaaagaagcc gaaaaaggaa
       610        620        630        640        650        660
gaggaacaga agtggaaatg gtgggaagaa gaacgttatc cagaaggcat caaatggaaa
       670        680        690        700        710        720
ttcctagagc ataaagggcc tgtcttcgct ccaccatatg agcctctgcc agagagtgtc
       730        740        750        760        770        780
aagttttact atgatggtaa agttatgaag ctgagtccta aagcagaaga agtagctaca
       790        800        810        820        830        840
ttctttgcaa aaatgcttga ccacgaatat actactaagg aaatattcag gaaaaatttc
       850        860        870        880        890        900
tttaaagatt ggagaaagga aatgactaat gacgaaaaaa atacgattac caacctaagc
       910        920        930        940        950        960
aaatgcgact ttacacagat gagccagtat ttcaaagcgc agtcagaagc tcggaaacag
       970        980        990       1000       1010       1020
atgagcaagg aagaaaaatt gaaaatcaaa gaagaaaatg aaaagttatt gaaagaatac
      1030       1040       1050       1060       1070       1080
ggcttttgtg taatggataa tcatagagag cggattgcca acttcaagat agagcctccg
      1090       1100       1110       1120       1130       1140
gggcttttcc gaggccgagg gaaccacccc aagatgggta tgctgaagag aaggatcatg
      1150       1160       1170       1180       1190       1200
cctgaggaca tcatcatcaa ctgtagcaaa gacgcaaagg ttccttctcc ccctcctgga
      1210       1220       1230       1240       1250       1260
cataaatgga aggaagtccg acatgataac aaggttactt ggctggtctc ctggacagag
      1270       1280       1290       1300       1310       1320
aatatccaag gttctatcaa atatatcatg ctgaatccca gttcacgaat caagggtgag
      1330       1340       1350       1360       1370       1380
aaagactggc agaagtatga gactgcgcgg cgtctgaaga agtgtgtgga caagatccga
      1390       1400       1410       1420       1430       1440
aatcagtatc gggaagactg gaagtccaaa gagatgaaag ttcgtcagag agctgtagca
      1450       1460       1470       1480       1490       1500
ctgtacttca ttgataagct tgctctgaga gcaggcaatg agaaggaaga aggagagacg
      1510       1520       1530       1540       1550       1560
gcagacactg tgggctgctg ttcacttaga gtggaacaca tcaatttaca cccagaattg
      1570       1580       1590       1600       1610       1620
gatggtcagg agtatgtggt ggaatttgac tttcctggga aggactcaat cagatactat
      1630       1640       1650       1660       1670       1680
aacaaagtcc cagttgagaa acgagttttt aaaaacttac aactgtttat ggagaacaag
      1690       1700       1710       1720       1730       1740
cagcctgagg acgatctttt tgatcgactt aatactggta ttctaaataa acatcttcag
      1750       1760       1770       1780       1790       1800
gatctcatgg agggcttgac tgccaaggtg ttccgtacct acaatgcctc catcacacta
      1810       1820       1830       1840       1850       1860
cagcagcagc ttaaagagct cacagcccct gatgagaatg taccagcgaa gattctatct
      1870       1880       1890       1900       1910       1920
tataaccgtg ccaatcgagc tgttgcaatt ctttgtaacc accagagggc gccaccaaag
      1930       1940       1950       1960       1970       1980
acctttgaga agtcaatgat gaacttgcag tctaagattg atgccaagaa agatcagtta
      1990       2000       2010       2020       2030       2040
gcagatgctc gaagggacct gaaaagtgct aaggctgatg ccaaagtcat gaaggatgca
      2050       2060       2070       2080       2090       2100
aagaccaaga aggtagtaga gtcaaaaaag aaggctgtac agagactaga agagcagttg
      2110       2120       2130       2140       2150       2160
atgaagctgg aagttcaagc cacagatcga gaggagaaca aacaaattgc cttggggacc
      2170       2180       2190       2200       2210       2220
tccaaactca attatctgga ccctaggatc acagtggctt ggtgcaaaaa gtggggggtc
      2230       2240       2250       2260       2270       2280
ccaattgaga agatttacaa caaaacccag agggagaagt ttgcttgggc cattgatatg
      2290       2300
actgatgagg actacgagtt ttga   

Last modification date: Oct. 2, 2011