Schizo-saccharomyces pombe Lindner (fission yeast) [SPO]

FULL NAME: DNA topoisomerase 1


DESCRIPTION:
The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.

MISCELLANEOUS:
When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.


STRUCTURE SIMILARITY:
Belongs to the eukaryotic type I topoisomerase family.


CATALYTIC ACTIVITY:
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MSSSDSDSVS LSIRRRQRRG SSKRISMKES DEESDSSENH PLSESLNKKS KSESDEDDIP
        70         80         90        100        110        120
IRKRRASSKK NMSNSSSKKR AKVMGNGGLK NGKKTAVVKE EEDFNEIAKP SPKHKRVSKA
       130        140        150        160        170        180
NGSKNGAKSA VKKEESDTDD SVPLRAVSTV SLTPYKSELP SGASTTQNRS PNDEEDEDED
       190        200        210        220        230        240
YKWWTSENID DTQKWTTLEH NGVIFAPPYE PLPKNVKLIY DGNPVNLPPE AEEVAGFYAA
       250        260        270        280        290        300
MLETDHAKNP VFQDNFFRDF LKVCDECNFN HNIKEFSKCD FTQMFHHFEQ KREEKKSMPK
       310        320        330        340        350        360
EQKKAIKQKK DEEEEKYKWC ILDGRKEKVG NFRIEPPGLF RGRGSHPKTG SLKRRVYPEQ
       370        380        390        400        410        420
ITINIGEGVP VPEPLPGHQW AEVKHDNTVT WLATWHENIN NNVKYVFLAA GSSLKGQSDL
       430        440        450        460        470        480
KKYEKSRKLK DYIDDIRKGY RKDLKSELTV ERQRGTAMYL IDVFALRAGN EKGEDEADTV
       490        500        510        520        530        540
GCCSLRYEHV TLKPPRTVVF DFLGKDSIRY YNEVEVDPQV FKNLKIFKRP PKKEGDLIFD
       550        560        570        580        590        600
RLSTNSLNKY LTSLMDGLSA KVFRTYNASY TMAEELKKMP KNLTLADKIL FYNRANRTVA
       610        620        630        640        650        660
ILCNHQRSVT KNHDVQMERF AERIKALQYQ RMRLRKMMLN LEPKLAKSKP ELLAKEEGIT
       670        680        690        700        710        720
DSWIVKHHET LYELEKEKIK KKFDRENEKL AAEDPKSVLP ESELEVRLKA ADELKKALDA
       730        740        750        760        770        780
ELKSKKVDPG RSSMEQLEKR LNKLNERINV MRTQMIDKDE NKTTALGTSK INYIDPRLTY
       790        800        810
SFSKREDVPI EKLFSKTIRD KFNWAADTPP DWKW  

Encoded by top1 gene


Nucleic acid sequence

        10         20         30         40         50         60
atgtcttcgt ctgattcaga ttccgtgtct ttatccatta gaaggaggca aagacgcggt
        70         80         90        100        110        120
tcatctaaac ggattagtat gaaggagtcg gacgaagaga gtgatagttc tgaaaatcat
       130        140        150        160        170        180
cctcttagtg aatcgttgaa taagaaatcc aaatccgaga gtgatgagga tgatataccg
       190        200        210        220        230        240
attagaaaga gacgtgcttc ttcaaagaaa aatatgtcca attcaagctc taaaaaaaga
       250        260        270        280        290        300
gctaaagtaa tgggaaatgg tggtttaaaa aacggaaaaa aaacggcggt tgtaaaagag
       310        320        330        340        350        360
gaagaggatt tcaatgaaat tgcaaagccc tcaccgaagc ataaacgtgt ttctaaagcg
       370        380        390        400        410        420
aatggaagta aaaatggcgc gaagtcagct gttaaaaaag aggaaagtga cacagatgat
       430        440        450        460        470        480
tcagttccat tgagagctgt ttcgactgta tctttaactc cttacaaatc agaactgcct
       490        500        510        520        530        540
tcgggagctt cgaccacgca aaatcgctca ccaaacgatg aggaagatga agatgaggat
       550        560        570        580        590        600
tacaagtggt ggacttcaga aaatatcgat gatactcaaa aatggactac attggagcat
       610        620        630        640        650        660
aatggtgtaa tttttgctcc accctatgaa cctttaccaa agaacgtcaa gctaatttac
       670        680        690        700        710        720
gatggaaacc ccgtaaatct tcctcccgaa gcagaagaag ttgctggttt ttatgctgca
       730        740        750        760        770        780
atgcttgaaa ccgatcatgc caaaaatcct gtattccaag ataatttttt ccgtgacttc
       790        800        810        820        830        840
ttaaaggtct gtgatgaatg taactttaat cacaacatta aagagttttc taaatgtgat
       850        860        870        880        890        900
tttacccaaa tgtttcacca ttttgagcaa aagagggaag agaagaagag tatgccgaag
       910        920        930        940        950        960
gaacagaaga aggcaataaa gcagaaaaaa gatgaggaag aggaaaaata taaatggtgc
       970        980        990       1000       1010       1020
atacttgatg ggagaaagga gaaggttggt aactttcgta ttgaacctcc agggttattt
      1030       1040       1050       1060       1070       1080
cgtggtcgag gtagtcatcc taaaactggt tctttaaagc gtcgagtata tcctgaacaa
      1090       1100       1110       1120       1130       1140
attaccatta atattggtga aggtgtaccc gttccagaac cactccctgg gcatcaatgg
      1150       1160       1170       1180       1190       1200
gcggaggtaa agcatgataa tacagtgacc tggttggcaa cctggcatga aaatataaat
      1210       1220       1230       1240       1250       1260
aataatgtca aatatgtctt tttagctgca ggaagttctc taaaaggaca gagtgactta
      1270       1280       1290       1300       1310       1320
aaaaaatacg aaaagtcaag aaagcttaag gattatattg atgatatccg taaaggctac
      1330       1340       1350       1360       1370       1380
cggaaagatt tgaaaagtga gttaacggtt gagcgtcaaa gaggaactgc catgtattta
      1390       1400       1410       1420       1430       1440
attgatgttt ttgctttaag agcaggaaat gaaaagggtg aagacgaggc ggatactgtt
      1450       1460       1470       1480       1490       1500
ggttgttgtt cactgcgata tgaacatgtt acactgaagc caccacgaac agtcgttttc
      1510       1520       1530       1540       1550       1560
gattttcttg gtaaagattc tattcgttac tacaacgagg ttgaagttga tccccaagtt
      1570       1580       1590       1600       1610       1620
tttaaaaatc taaagatctt taaacgtcct cccaaaaaag agggtgattt aattttcgac
      1630       1640       1650       1660       1670       1680
cgtctcagta caaacagtct taacaaatat ctgactagcc ttatggatgg actttcagct
      1690       1700       1710       1720       1730       1740
aaagtatttc gtacctacaa tgcttcatac accatggccg aggaacttaa gaaaatgcct
      1750       1760       1770       1780       1790       1800
aagaacctca cccttgcaga caaaatacta ttttataata gggcaaatag gactgttgca
      1810       1820       1830       1840       1850       1860
attttatgta atcatcaacg ttccgtaacc aaaaatcacg atgttcaaat ggaacggttt
      1870       1880       1890       1900       1910       1920
gccgaaagga ttaaggcatt acaataccag cggatgagac tgcgaaaaat gatgctgaat
      1930       1940       1950       1960       1970       1980
ttagagccca agcttgctaa aagcaagccc gaattgctgg ctaaagaaga aggcattacc
      1990       2000       2010       2020       2030       2040
gattcatgga tcgtaaaaca tcacgagaca ctttacgaac tagaaaaaga gaaaataaaa
      2050       2060       2070       2080       2090       2100
aagaaattcg atcgtgagaa cgaaaaatta gctgctgagg atcccaaatc agtgcttccg
      2110       2120       2130       2140       2150       2160
gaatctgaat tggaagttcg attgaaagcg gctgatgagt tgaagaaagc gctggacgct
      2170       2180       2190       2200       2210       2220
gaacttaaaa gcaaaaaagt cgatccaggt cgttcttcga tggaacaact tgagaaaaga
      2230       2240       2250       2260       2270       2280
ttaaacaaac tcaatgaacg aattaatgtt atgcgtactc agatgatcga taaagacgag
      2290       2300       2310       2320       2330       2340
aataaaacta ctgctttggg tacaagtaag attaactaca tagacccgag gcttacttat
      2350       2360       2370       2380       2390       2400
tcgttcagca agcgagaaga cgttcctatt gagaagctgt ttagtaagac gattcgtgac
      2410       2420       2430       2440
aagttcaatt gggctgctga tacacctccg gattggaagt ggtaa 

Last modification date: Oct. 2, 2011