Drosophila melanogaster Meigen (fruit fly) [DME]

FULL NAME: DNA topoisomerase 1


DESCRIPTION:
The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.

MISCELLANEOUS:
When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.


STRUCTURE SIMILARITY:
Belongs to the eukaryotic type I topoisomerase family.


CATALYTIC ACTIVITY:
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.


RELATED PATHWAY(S):
DNA replication


Amino acids sequence

        10         20         30         40         50         60
MSGDVAAENS IHIQNGGSCE VVQSNGVTTN GHGHHHHHHH SSSSSSSSKH KSSSKDKHRD
        70         80         90        100        110        120
REREHKSSNS SSSSKEHKSS SRDKDRHKSS SSSSKHRDKD KERDGSSNSH RSGSSSSHKD
       130        140        150        160        170        180
KDGSSSSKHK SSSGHHKRSS KDKERRDKDK DRGSSSSSRH KSSSSSRDKE RSSSSHKSSS
       190        200        210        220        230        240
SSSSSKSKHS SSRHSSSSSS KDQPSYDGVF VKPEPVSQQL MHSGSVDAFQ MQQLGSYEAA
       250        260        270        280        290        300
AAGTNFNGNG NVAGANYKNG YEESIVDIKK EEESFNNLSQ ASSCDYSMSQ FRADEPPFVV
       310        320        330        340        350        360
KHEQSYAEED STMNYNDHDD DADEMNDDEE DVPLAMRKRK QEATDRPDGG MDNDDDDDIP
       370        380        390        400        410        420
LLARKKVKKE KIKKESKEKS KKRVKEEPSD DYGNVKPKKK KMKKEPEPAV SPGKRQKAKA
       430        440        450        460        470        480
KVEEEEVWRW WEEEKRADGV KWSTLEHKGP VFAPRYERVP RNVRFYYDGK PLELSEETEE
       490        500        510        520        530        540
AATFYAKMLN HDYCTKEVFN NNFFKDFRKS MTPREREIIK DFRKCNFQEM FNYFQAESEK
       550        560        570        580        590        600
RKAASKEEKL IKKNENEALM KEFGFCMIDG HKEKIGNFRL EPPGLFRGRG EHPKMGMIKR
       610        620        630        640        650        660
RIQASDVSIN CGKDSKVPSP PPGSRWKEVR HDNTVTWLAS WIENVQGQVK YIMLNPSSKL
       670        680        690        700        710        720
KGEKDHIKYE TARRLDKVID KIRATYRDEW KSKEMRVRQR AVALYFIDKL ALRAGNEKDE
       730        740        750        760        770        780
DQADTVGCCS LRVEHVQLHK ELNGKENVVV FDFPGKDSIR YYNEVEVEKR VFKNLELFME
       790        800        810        820        830        840
HKKEGDDLFD RLNTQVLNEH LKELMEGLTA KVFRTYNASK TLQSQLDLLT DPSATVPEKL
       850        860        870        880        890        900
LAYNRANRAV AILCNHQRSV PKSHEKSMEN LKEKIKAKRE AIEKCESEYH SRDEKKGKQL
       910        920        930        940        950        960
ERLRDQLKKL ELQETDRDEN KTIALGTSKL NYLDPRISVA WCKKHDVPIE KIFNKTQRTK
       970
FLWAVHMADE NYRF    

Encoded by Top1 gene

FULL NAME: Topoisomerase 1


OTHER NAME(S):
CG6146
Dmel\CG6146
dTopoI
l(1)G0134
l(1)G0201
l(1)G0229
l(1)G0278
l(1)top1
top1
TOP1
TopI
topo I
Topo I
topoI
TopoI


Nucleic acid sequence

        10         20         30         40         50         60
atgagtgggg atgtggctgc cgaaaatagc atccacatac aaaatggtgg cagctgcgag
        70         80         90        100        110        120
gtggttcaat ccaacggggt gaccaccaat ggacacggac accaccacca tcatcaccac
       130        140        150        160        170        180
agcagcagca gcagcagcag cagcaagcat aaatcctcca gcaaggacaa gcatcgtgat
       190        200        210        220        230        240
agggagaggg agcacaagag ctccaattcc tccagctcgt cgaaggagca caagagcagc
       250        260        270        280        290        300
agtcgcgaca aggatcgaca caagagcagc agttcgtcgt caaagcatcg ggacaaggac
       310        320        330        340        350        360
aaggagcgcg atggcagcag caactcgcat cgcagcggct catcgtccag ccacaaggat
       370        380        390        400        410        420
aaggatggga gcagcagcag caagcacaag tcgtcgtcgg gccaccacaa gaggtcctcg
       430        440        450        460        470        480
aaggataagg agcgacgcga taaggacaag gatcgcggca gcagttcatc cagtcggcac
       490        500        510        520        530        540
aagtcctcgt ccagctcccg tgacaaagag cgcagcagca gcagtcacaa gagcagctcc
       550        560        570        580        590        600
tcttcatcct catcgaagag caaacattcc agttcgcggc acagcagctc atcttcctcg
       610        620        630        640        650        660
aaagaccagc catcgtacga tggcgtattt gttaagccgg agcccgtttc ccaacagctg
       670        680        690        700        710        720
atgcactcgg gctcggtgga tgctttccag atgcaacagc tcggtagcta cgaagccgca
       730        740        750        760        770        780
gccgccggca ccaattttaa tggcaatgga aacgttgccg gtgccaacta taagaacggc
       790        800        810        820        830        840
tacgaggaat cgatcgtaga catcaagaag gaggaggaga gctttaacaa tctgtcgcaa
       850        860        870        880        890        900
gccagctcct gtgactactc catgtcccag tttcgtgccg atgagccgcc gtttgtggtg
       910        920        930        940        950        960
aagcacgagc agagctacgc cgaagaggac agcaccatga actacaatga tcacgacgac
       970        980        990       1000       1010       1020
gacgccgacg agatgaacga cgacgaggag gatgtgccgc tggccatgcg caagcgcaaa
      1030       1040       1050       1060       1070       1080
caggaggcaa ccgatcgtcc tgatggcggc atggataacg acgatgacga cgacattccg
      1090       1100       1110       1120       1130       1140
ctgctggcgc gcaagaaggt taagaaggag aagatcaaga aggagtccaa ggaaaagtcc
      1150       1160       1170       1180       1190       1200
aagaagcggg tcaaggagga gcccagcgat gattacggca atgtcaagcc gaaaaagaaa
      1210       1220       1230       1240       1250       1260
aagatgaaaa aggagcccga gcctgccgta tcccccggca aacgacagaa ggcaaaggcc
      1270       1280       1290       1300       1310       1320
aaggtggagg aggaggaggt ttggcgatgg tgggaggagg agaagcgtgc cgatggcgtc
      1330       1340       1350       1360       1370       1380
aaatggtcaa cgctggagca caagggaccc gtgtttgcac cgcgatacga acgggtgcca
      1390       1400       1410       1420       1430       1440
cgcaatgtac ggttctacta cgatggcaag ccgttggagc tctctgagga aacggaggag
      1450       1460       1470       1480       1490       1500
gcagccacgt tctatgccaa gatgctgaac cacgactact gcaccaagga agtgttcaat
      1510       1520       1530       1540       1550       1560
aacaacttct tcaaggactt ccgcaagtcc atgacgccca gagagaggga gattatcaag
      1570       1580       1590       1600       1610       1620
gatttccgca agtgcaactt ccaggagatg ttcaactact tccaagcgga gtccgaaaag
      1630       1640       1650       1660       1670       1680
cgcaaggcag ccagcaagga ggagaagctg atcaagaaga acgaaaacga ggcgctgatg
      1690       1700       1710       1720       1730       1740
aaggaattcg gattctgcat gattgatggg cacaaggaga agatcggtaa cttccgcctg
      1750       1760       1770       1780       1790       1800
gagccgccag gcctgttccg cggtcgtggc gagcatccca aaatgggcat gatcaagcgg
      1810       1820       1830       1840       1850       1860
cgcatccaag ccagcgatgt gtccattaac tgtggcaaag attcaaaggt gccttcaccg
      1870       1880       1890       1900       1910       1920
ccgcccggat ctcgctggaa ggaggtgcgc cacgacaaca cggtcacctg gctggcctcc
      1930       1940       1950       1960       1970       1980
tggatcgaga atgtgcaggg acaggtcaag tacatcatgt tgaatccctc ctcgaaactc
      1990       2000       2010       2020       2030       2040
aagggcgaga aggatcacat taagtacgag acggcgcgac gcctggacaa ggtaatcgat
      2050       2060       2070       2080       2090       2100
aagatacgtg ccacctatcg cgacgagtgg aagtccaagg agatgagggt tcgccagcga
      2110       2120       2130       2140       2150       2160
gcggtggccc tttacttcat cgacaaactg gcgctgagag caggcaacga aaaggacgag
      2170       2180       2190       2200       2210       2220
gatcaggccg ataccgtggg ctgttgctcg ctacgcgtgg agcatgtcca gctccacaag
      2230       2240       2250       2260       2270       2280
gaactgaacg gaaaggagaa cgtggtggtc tttgatttcc ccggtaagga ctccattcgg
      2290       2300       2310       2320       2330       2340
tattacaacg aggttgaggt ggagaagcgc gtctttaaga acctcgaact gttcatggag
      2350       2360       2370       2380       2390       2400
cacaagaagg agggcgacga cctcttcgat cgtctcaaca cgcaggtgct taatgagcat
      2410       2420       2430       2440       2450       2460
ctcaaggagc tgatggaagg tctcacggcc aaggtattcc gtacgtacaa cgcatcgaaa
      2470       2480       2490       2500       2510       2520
acactgcaaa gtcagctgga tctgttgacc gatccgagcg ccacggtacc ggaaaagctg
      2530       2540       2550       2560       2570       2580
ttggcctaca atcgcgccaa ccgtgccgtg gccatcctct gtaaccatca gcgttccgtg
      2590       2600       2610       2620       2630       2640
cccaagagcc acgagaagtc aatggagaat ctgaaggaga agatcaaggc caagcgggaa
      2650       2660       2670       2680       2690       2700
gccatcgaaa aatgcgagtc cgagtatcat tcgagagacg agaagaaggg caaacagctg
      2710       2720       2730       2740       2750       2760
gagcgcctaa gggatcagct gaagaaacta gaactacaag agactgatag agacgagaat
      2770       2780       2790       2800       2810       2820
aagaccattg ccctgggcac atctaagcta aactatcttg atccacgcat atcggtggct
      2830       2840       2850       2860       2870       2880
tggtgtaaaa agcatgacgt gccgattgag aagatattca acaaaacgca aagaaccaaa
      2890       2900       2910       2920
tttctgtggg ccgttcatat ggccgatgag aattatcgtt tctaa 

Last modification date: Oct. 2, 2011